Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntze) as revealed by inter simple sequence repeats

Thomas, Jibu; Vijayan, Deepu; Joshi, S.; Lopez, S. Joseph; Kumar, Rahul

doi:10.1016/j.jbiotec.2005.11.005

Cited by 41 publications

(17 citation statements)

References 18 publications

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“…In comparison to other approaches, the application of ISSR, RAPD, and RFLP markers, 4.36% of variation in somatic embryo-derived plantlets of coffee was revealed (Rani et al 2000). ISSR markers appeared to be highly efficient in the detection of variation (50%) among somaclones derived from single cell line originating from a cotyledonary culture of tea (Thomas et al 2006). Whereas, the simple sequence repeats successfully detected about 31% of chimeric mutations that were assigned to either DNA polymerase slippage effect or chromosome loss in cacao SE regenerants (Rodriguez Lopez et al 2004).…”

Section: Discussionmentioning

confidence: 91%

Flow Cytometry, HPLC-RP, and metAFLP Analyses to Assess Genetic Variability in Somatic Embryo-Derived Plantlets of Gentiana pannonica Scop

2010

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Cytometric and molecular techniques were used to verify genetic uniformity among somatic embryo-derived plantlets of Gentiana pannonica Scop. Cytometric analysis of regenerants revealed absence of chromosomal changes and alterations in ploidy. However, reverse phase high pressure liquid chromatography detected higher levels of methylation in regenerated plants than those of control plants. These changes were further investigated using a quantitative molecular marker-based approach. This revealed that numerous tissue culture-induced variations, ∼3% (epi)mutations, were observed, including sequence variation and changes in methylation patterns. Moreover, complex patterns of variation, including combinations of genetic and epigenetic changes, were relatively high (ca. 9%). Overall, tissue culture-induced variation reached 16%; while, demethylation was lower than de novo methylation in heterozygotic material and similar in all regenerated plantlets.

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Section: Discussionmentioning

confidence: 91%

Flow Cytometry, HPLC-RP, and metAFLP Analyses to Assess Genetic Variability in Somatic Embryo-Derived Plantlets of Gentiana pannonica Scop

2010

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“…ISSR is a useful marker system which has been successfully employed to measure genetic stability/instability in tea (Thomas et al, 2006), Prunus mume (Ning et al, 2007), Swertia chirayita Dhawan, 2007), A. albus (Hu et al, 2008), London plane tree (Huang et al, 2008) and gerbera (Bhatia et al, 2009). It has several obvious advantages over random amplified polymorphic DNA (RAPD), such as higher reproducibility and informativeness (Nagaoka and Ogihara, 1997), which make ISSR more attractive for genotyping and diversity studies.…”

Section: Resultsmentioning

confidence: 99%

ISSR Analysis of Somaclonal Variation in Callus-Derived Plants of Amorphophallus Rivieri Durieu

Hu¹,

Li²,

Li³

2011

Acta Biologica Cracoviensia Series Botanica

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This communication reports detection of somaclonal variation among tissue culture-raised plants of Amorphophallus rivieri Durieu, an economically important crop in China, with high content of glucomannan in its corms. A population of regenerated plants was obtained from a single donor plant of A. rivieri via corm organogenesis, and 28 plants were randomly selected as a representative sample and subjected to analysis of somaclonal variation using inter-simple sequence repeat (ISSR) markers. Of the 26 ISSR primers screened, 13 gave distinct and reproducible band patterns, yielding 131 bands with an average of 10.1 bands per primer. Ten primers were polymorphic and generated 16 polymorphic bands with 12.2% mean polymorphism. Based on the ISSR data from the regenerated plants and the donor plant, Jaccard's similarity coefficients were calculated; they ranged from 0.961 to 1.000 with a mean of 0.982. A dendrogram was constructed using the unweighted pair group method with arithmetic mean (UPGMA); it showed that a majority of regenerated plants (including the donor plant) clustered closely, with a mean similarity coefficient of 0.987. Low somaclonal variation observed in the regenerated plants indicates that rapid propagation of A. rivieri via corm organogenesis is a practicable method with a low risk of genetic instability.K Ke ey y w wo or rd ds s: : Amorphophallus rivieri Durieu, ISSR marker, regenerated plants, somaclonal variation.

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“…at the species level; mean PPB was 21.4-50%, and mean I was 0.229 at the population level (Kaundun et al, 2000;Kumar, 2002;Chen et al, 2005;Thomas et al, 2006). In the present study, PPB was 88.89%, H was 0.2809, and I was 0.4278 at the species level; mean PPB was 42.12%, mean H was 0.1373, and I was 0.2058 at population level.…”

Section: Discussionmentioning

confidence: 99%

Genetic diversity and differentiation in Camellia reticulata (Theaceae) polyploid complex revealed by ISSR and ploidy

Wang

Ruan²

2012

Genet. Mol. Res.

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ABSTRACT. Camellia reticulata is a well-known ornamental and oil plant that is endemic to southwest China. This species shows three cell ploidies, i.e., diploidy, tetraploidy and hexaploidy. We made the first investigation of genetic diversity and differentiation of natural populations of C. reticulata, and 114 individuals from 6 populations were sampled. Cytogeography results showed that ploidy is invariable within populations and evenly distributed. A relatively high level of genetic diversity was found in C. reticulata, both at the species level (PPB = 88.89%; H = 0.2809; I = 0.4278) and at the population level (mean PPB = 42.13%; mean H = 0.14; mean I = 0.21). We found a relatively low degree of differentiation among ploidies (G ST = 0.2384; AMOVA = 10.26%) and a relatively high degree of differentiation among populations (G CS = 0.3807; AMOVA = 48.75%). The high genetic diversity can be explained by its biological char acter, wide distribution and ploidies, and the special genetic structure can be ascribed to polyploid origin from hybridization with different Camellia spp. This information will be useful for the introduction, conservation and further studies of C. reticulata and related species.

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Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntze) as revealed by inter simple sequence repeats

Cited by 41 publications

References 18 publications

Flow Cytometry, HPLC-RP, and metAFLP Analyses to Assess Genetic Variability in Somatic Embryo-Derived Plantlets of Gentiana pannonica Scop

Flow Cytometry, HPLC-RP, and metAFLP Analyses to Assess Genetic Variability in Somatic Embryo-Derived Plantlets of Gentiana pannonica Scop

ISSR Analysis of Somaclonal Variation in Callus-Derived Plants of Amorphophallus Rivieri Durieu

Genetic diversity and differentiation in Camellia reticulata (Theaceae) polyploid complex revealed by ISSR and ploidy

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