Genetic instability and aging under the scrutiny of comparative biology: a meta-analysis of spontaneous micronuclei frequency

Croco, Eleonora; Marchionni, Silvia; Lorenzini, Antonello

doi:10.1016/j.mad.2016.04.004

Cited by 10 publications

(9 citation statements)

References 59 publications

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“…Making these observations particularly relevant in this context is that we did not observe a meaningful relationship of this capacity with adult body mass, despite the fact that the evolution of large body mass would be expected to positively associate with increased genomic stability (reviewed in Croco et al [56]). We have also demonstrated, in fact, a relationship between body mass and a more efficient erythropoiesis in mammals [57].…”

Section: Molecular Level Telomeressupporting

confidence: 58%

Development and Longevity: Cellular and Molecular Determinants – A Mini-Review

2020

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Across species, development and longevity are tightly linked. We discuss the relevant literature and suggest that the root for this stringent relationship is the rate of development. The basis for the relationship between rate of development and longevity lies in adaptations that have occurred through evolution at multiple levels of biological complexity: organism, organ, cellular, and molecular. Thus, the analysis of the relationship is of interest for multiple fields of biology.

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Section: Molecular Level Telomeressupporting

confidence: 58%

Development and Longevity: Cellular and Molecular Determinants – A Mini-Review

2020

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“…With the observation of the exceptional longevity of the ocean quahog Arctica islandica (Ridgway & Richardson 2011), bivalves are becoming also an attractive model for ageing studies (Abele et al 2009). Comparative biology of longevity determinants is an informative field that is progressively expanding its observations at the cellular level (Stuart et al 2013;Croco et al 2016Croco et al , 2017b.…”

Section: Introductionmentioning

confidence: 99%

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

Attaallah

Marchionni

El-Beltagy

et al. 2020

The European Zoological Journal

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A huge progress has been achieved in mammalian in vitro technique. Instead, of the many trials to develop marine invertebrate cell cultures, only a few have obtained them and only from few tissues. Since in vitro cell culture of invertebrates could be very useful for many aspects of basic and applied science, in this work we investigate and describe the development of a technique for the establishment of cell cultures from gill, mantle and gonadic tissue of the Manila clam (Ruditapes philippinarum). We maintained viable cultures for up to 25 days. Culture viability and proliferation were tested with 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) and with trypan blue, while an antibody against the ATP-dependent RNA helicase VASA, a protein expressed in the germline, and in multipotent stem cells of some animals, was used to verify the presence of these cell types. Following the described protocol: 1) explant resulted the better source to obtain cell cultures, when compared to enzymatic dissociation; 2) cultures of suspended cells were viable for longer period than adherent cells; 3) cell cultures obtained from tissues sampled in September-October performed better compared to other periods of the year, regarding maintenance and growth; 4) the tissue from which we obtained longer-lived cell cultures was gonadic tissue, especially form samples that show more undifferentiated germ cells and more VASA-stained cells. This study describes the challenges concerning the development of in vitro culture techniques for aquatic invertebrates.

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“…The effect of cytotoxic drugs and radiation can also be studied by following the number of DNA damage foci. For example, the morphology and number of DNA repair foci were investigated by [82]. These authors showed that cells of longer-lived species exhibit a higher number of 53BP1-positive foci than cells of shorter-lived species.…”

Section: Introductionmentioning

confidence: 99%

A role of the 53BP1 protein in genome protection: structural and functional characteristics of 53BP1-dependent DNA repair

Bártová

Legartová

Dundr

et al. 2019

Aging

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Nuclear architecture plays a significant role in DNA repair mechanisms. It is evident that proteins involved in DNA repair are compartmentalized in not only spontaneously occurring DNA lesions or ionizing radiation-induced foci (IRIF), but a specific clustering of these proteins can also be observed within the whole cell nucleus. For example, 53BP1-positive and BRCA1-positive DNA repair foci decorate chromocenters and can appear close to nuclear speckles. Both 53BP1 and BRCA1 are well-described factors that play an essential role in double-strand break (DSB) repair. These proteins are members of two protein complexes: 53BP1-RIF1-PTIP and BRCA1-CtIP, which make a “decision” determining whether canonical nonhomologous end joining (NHEJ) or homology-directed repair (HDR) is activated. It is generally accepted that 53BP1 mediates the NHEJ mechanism, while HDR is activated via a BRCA1-dependent signaling pathway. Interestingly, the 53BP1 protein appears relatively quickly at DSB sites, while BRCA1 is functional at later stages of DNA repair, as soon as the Mre11-Rad50-Nbs1 complex is recruited to the DNA lesions. A function of the 53BP1 protein is also linked to a specific histone signature, including phosphorylation of histone H2AX (γH2AX) or methylation of histone H4 at the lysine 20 position (H4K20me); therefore, we also discuss an epigenetic landscape of 53BP1-positive DNA lesions.

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Genetic instability and aging under the scrutiny of comparative biology: a meta-analysis of spontaneous micronuclei frequency

Cited by 10 publications

References 59 publications

Development and Longevity: Cellular and Molecular Determinants – A Mini-Review

Development and Longevity: Cellular and Molecular Determinants – A Mini-Review

Cell cultures of the Manila clam and their possible use in biomonitoring and species preservation

A role of the 53BP1 protein in genome protection: structural and functional characteristics of 53BP1-dependent DNA repair

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