Genetic Inactivation of Dace, Leuciscus Leuciscus (L.), Gametes Using UV Irradiation

Kucharczyk, Dariusz; Targońska, Katarzyna; Szczerbowski, Andrzej; Luczynski, M.; Rozek, Wojciech; Kujawa, Roman; Mamcarz, Andrzej

doi:10.2478/s10086-008-0030-4

Cited by 7 publications

(8 citation statements)

References 19 publications

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“…Similar data to the present work have been reported by other authors: i.e. Scheerer et al (1986) and Babiak et al (2002aBabiak et al ( , 2002b for rainbow trout (Oncorhynchus mykiss Walbaum 1792), May et al (1988) for brook trout (Salvelinus fontinalis Mitchill 1814), Arai et al (1992) for loach (Misgurnus anguillicaudatus Cantor 1842), Bongers et al (1994) for common carp, Kucharczyk (2002) for common bream, Lin and Dabrowski (1998) for northern pike and Kucharczyk (2001) and Kucharczyk et al (2008bKucharczyk et al ( , 2008c for ide (Leuciscus idus L.) and dace (Leuciscus leuciscus L.). Such low survival rates of androgenotes were probably connected with the synergic effect of a few sublethal manipulations such as irradiation of oocytes and applied temperature shock as well as increased inbreeding.…”

Section: Resultssupporting

confidence: 90%

“…The applied dose of UV irradiation was 3456 J m -2 , at which almost 100% haploid embryos were produced at a hatching rate of over 2%. These doses were higher than those described as the optimum UV oocyte treatment for common carp (2500 J m -2 ) by Bongers et al (1994) or for northern pike (660 to 1320 Jm -2 ) by Lin and Dabrowski (1998) and were similar to those reported by Kucharczyk (2002) for common bream and Kucharczyk (2001) for ide (2700 to 3500 J m -2 ). For doubling chromosomal material during the androgenesis process, heat shocks were usually applied and, in some cases, very intensively (up to 42°C) (Arai et al, 1995;Grunina et al, 1995;Bercsenyi et al, 1998;Pandian and Koteeswaran, 1998;Kucharczyk, 2002).…”

Section: Resultssupporting

confidence: 74%

“…One solution to the problem is to apply modern genome engineering. The aquaculture of many more species was dynamically powered to the developing genome engineering (Krasznai and Marian, 1986;Goryczko et al, 1991;Kucharczyk et al, 2008bKucharczyk et al, , 2008cOcalewicz et al, 2010) and the results of the studies are used not only for commercial purposes but are also of scientific interest. The low hatching rate of tench embryos from genetically inactivated oocytes (0-2%) has been observed in many other fish species.…”

Section: Resultsmentioning

confidence: 99%

“…The application of gamma or X radiation involves technical difficulties, including safety measures (Arai et al, 1992) and residual chromosome fragments in the oocyte (Ocalewicz et al, 2010). UV irradiation has been used successfully for genetic inactivation of cyprinid oocytes (Bongers et al, 1994;Kucharczyk, 2001Kucharczyk, , 2002Kucharczyk et al, 2008bKucharczyk et al, , 2008c.…”

Section: Introductionmentioning

confidence: 99%

“…Initially, researchers focused on recovering stocks of breeding fish, although another aim of androgenesis may be the reconstruction of wild fish, which is possible when gene banks store the sperm of the extinct populations (Arai et al, 1992;Bongers et al, 1994;Flajshans, 1998;Kucharczyk, 2002). It is also possible to produce androgenetic offspring using eggs from other fish species (Grunina et al, 1995;Bercsenyi et al, 1998;Kucharczyk et al, 2008bKucharczyk et al, , 2008c. The common tench Tinca tinca (L.) is a species which has a huge commercial value in many European countries, such as the Czech Republic, Hungary, Italy, Spain and Poland and other continents (Wang et al, 2006;Celada et al, 2009;Kujawa et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Induced Artificial Androgenesis in Common Tench,Tinca Tinca(L.), Using Common Carp and Common Bream Eggs

Kucharczyk

Żarski

Targońska

et al. 2014

Italian Journal of Animal Science

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This study presents artificial induction using tench eggs, Tinca tinca (L.), of androgenetic origin. The oocytes taken from common bream, Abramis brama (L.) and common carp, Cyprinus carpio L. were genetically inactivated using UV irradiation and then inseminated using tench spermatozoa. Androgenetic origin (haploid or diploid embryos) was checked using a recessive colour (blond) and morphological markers. The percentage of hatched embryos in all experimental groups was much lower than in the control groups. All haploid embryos showed morphological abnormalities, which were recorded as haploid syndrome (stunted body, poorly formed retina, etc.). The optimal dose of UV irradiation of common bream and common carp eggs was 3456 J m -2 . At this dose, almost 100% of haploid embryos were produced at a hatching rate of over 6%. Lower UV-ray doses affected abnormal embryo development. The highest yield of tench androgenesis (about 2%) was noted when eggs were exposed to thermal shock 30 min after egg activation.

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Section: Resultssupporting

confidence: 90%

Section: Resultssupporting

confidence: 74%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Induced Artificial Androgenesis in Common Tench,Tinca Tinca(L.), Using Common Carp and Common Bream Eggs

Kucharczyk

Żarski

Targońska

et al. 2014

Italian Journal of Animal Science

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Cortical reaction as an egg quality indicator in artificial reproduction of pikeperch, Sander lucioperca

Żarski

Krejszeff²,

Palińska³

et al. 2012

Reprod. Fertil. Dev.

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The aim of this study was to investigate the process of the cortical reaction in eggs of pikeperch, Sander lucioperca (L.), as well as the application of microscopic assessment of this process in egg quality evaluation. The analysis was carried out with eggs obtained from 10 females by artificial reproduction, in which hormonal stimulation with hCG was applied. Subsequently, each sample of eggs (separately from each female fish) was analysed. The analysis included observation of the cortical reaction and the process of egg swelling, and determination of the effect of temperature (12, 14 and 16°C) and the presence of spermatozoa on the cortical reaction. The results indicate that the cortical reaction in pikeperch eggs is quite violent, resulting in visible deformation of eggs between 3 and 5 min after activation. No effect of temperature or the presence of spermatozoa on the cortical reaction was observed. A strong correlation was recorded for the percentage of egg deformations observed and embryo survival rate. The described method of determination of pikeperch egg quality (based on egg deformation rate between 3 and 5 min after activation) may be highly useful, both in scientific research (where high-quality eggs are required) and in hatchery practice.

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Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives

et al. 2014

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Androgenesis is a form of uniparental reproduction leading to progenies inheriting only the paternal set of chromosomes. It has been achieved with variable success in a number of freshwater species and can be attained by artificial fertilization of genetically inactivated eggs following exposure to gamma (γ), X-ray or UV irradiation (haploid androgenesis) and by restoration of diploidy by suppression of mitosis using a pressure or thermal shock. The conditions for the genetic inactivation of the maternal genome in the European sea bass (Dicentrarchus labrax L.) were explored using different combinations of UV irradiation levels and durations. UV treatments significantly affected embryo survival and generated a wide range of developmental abnormalities. Despite the wide range of UV doses tested (from 7.2 to 720 mJ.cm−2), only one dose (60 mJ.cm−2.min−1 with 1 min irradiation) resulted in a small percentage (14%) of haploid larvae at hatching in the initial trials as verified by flow cytometry. Microsatellite marker analyses of three further batches of larvae produced by using this UV treatment showed a majority of larvae with variable levels of paternal and maternal contributions and only one larva displaying pure paternal inheritance. The results are discussed also in the context of an assessment of the UV-absorbance characteristics of egg extracts in this species that revealed the presence of gadusol, a compound structurally related to mycosporine-like amino acids (MAAs) with known UV-screening properties.

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Genetic Inactivation of Dace, Leuciscus Leuciscus (L.), Gametes Using UV Irradiation

Cited by 7 publications

References 19 publications

Induced Artificial Androgenesis in Common Tench,Tinca Tinca(L.), Using Common Carp and Common Bream Eggs

Induced Artificial Androgenesis in Common Tench,Tinca Tinca(L.), Using Common Carp and Common Bream Eggs

Cortical reaction as an egg quality indicator in artificial reproduction of pikeperch, Sander lucioperca

Genetic Inactivation of European Sea Bass (Dicentrarchus labrax L.) Eggs Using UV-Irradiation: Observations and Perspectives

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