Genetic improvement of xylose metabolism by enhancing the expression of pentose phosphate pathway genes in <i>Saccharomyces cerevisiae</i> IR-2 for high-temperature ethanol production

Kobayashi, Yasuhiko; Sahara, Takehiko; Suzuki, Toshihiro; Kamachi, Saori; Matsushika, Akinori; Hashizume, Tamotsu; Ohgiya, Satoru; Kamagata, Yoichi; Fujimori, Kazuhiro E.

doi:10.1007/s10295-017-1912-5

Cited by 25 publications

(22 citation statements)

References 39 publications

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“…The tktA gene is a key gene in the PPP, which plays an important role in the efficiency of xylose fermentation. Similarly, Saccharomyces cerevisiae was used to ferment xylose at an ethanol yield of 0.51 g/L/h by the overexpression of tktA (encoding transketolase) [ 51 ]. In addition, tktA is a major cold-resistant gene [ 52 ].…”

Section: Discussionmentioning

confidence: 99%

Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics

Guo

Guan

et al. 2018

Biotechnol Biofuels

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BackgroundThe biological production of 2,3-butanediol from xylose-rich raw materials from Klebsiella pneumoniae is a low-cost process. RpoD, an encoding gene of the sigma factor, is the key element in global transcription machinery engineering and has been successfully used to improve the fermentation with Escherichia coli. However, whether it can regulate the tolerance in K. pneumoniae remains unclear.ResultsIn this study, the kpC mutant strain was constructed by altering the expression quantity and genotype of the rpoD gene, and this exhibited high xylose tolerance and 2,3-butanediol production. The xylose tolerance of kpC strain was increased from 75 to 125 g/L, and the yield of 2,3-butanediol increased by 228.5% compared with the parent strain kpG, reaching 38.6 g/L at 62 h. The RNA sequencing results showed an upregulated expression level of 500 genes and downregulated expression level of 174 genes in the kpC mutant strain. The pathway analysis further showed that the differentially expressed genes were mainly related to signal transduction, membrane transport, carbohydrate metabolism, and energy metabolism. The nine most-promising genes were selected based on transcriptome sequencing, and were evaluated for their effects on xylose tolerance. The overexpression of the tktA encoding transketolase, pntA encoding NAD(P) transhydrogenase subunit alpha, and nuoF encoding NADH dehydrogenase subunit F conferred increased xylose consumption and increased 2,3-butanediol production to K. pneumoniae.ConclusionsThese results suggest that the xylose tolerance and 2,3-butanediol production of K. pneumoniae can be greatly improved by the directed evolution of rpoD. By applying transcriptomic analysis, the upregulation of tktA, pntA, and nuoF that were coded are essential for the xylose consumption and 2,3-butanediol production. This study will provide reference for further research on improving the fermentation abilities by means of other organisms.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1312-8) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics

Guo

Guan

et al. 2018

Biotechnol Biofuels

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“…With development of new DNA editing technology, the metabolic potentials of microorganisms are being explored and harnessed in plenty new ways. The development of strains that can ferment xylose, the main pentose in coconut husk, is done by inserting genes related to the degradation pathway of this pentose, like overexpressing genes related to the pentose phosphate pathway (such as TKL1 and TAL1) [ 69 , 70 ]. Other strategies are decreasing the formation of xylitol as it is a harmful derivative for the complete fermentation of the pentoses and preventing the ubiquitination of hexose transporters, since they also act to carry pentoses, but suffer degradation when the concentration (or absence) of glucose decreases [ 71 , 72 ].…”

Section: Methods Used In Coconut Conversion To Ethanolmentioning

confidence: 99%

Second-Generation Bioethanol from Coconut Husk

Bolivar-Telleria

Turbay

Favarato

et al. 2018

BioMed Research International

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Coconut palm (Cocos nucifera) is an important commercial crop in many tropical countries, but its industry generates large amounts of residue. One way to address this problem is to use this residue, coconut husk, to produce second-generation (2G) ethanol. The aim of this review is to describe the methods that have been used to produce bioethanol from coconut husk and to suggest ways to improve different steps of the process. The analysis performed in this review determined that alkaline pretreatment is the best choice for its delignification potential. It was also observed that although most reported studies use enzymes to perform hydrolysis, acid hydrolysis is a good alternative. Finally, ethanol production using different microorganisms and fermentation strategies is discussed and the possibility of obtaining other added-value products from coconut husk components by using a biorefinery scheme is addressed.

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“…There is growing evidence to indicate that genetic background may determine whether the embryo can adapt to the teratogenic effects of ethanol exposure [42,43].…”

Section: Discussionmentioning

confidence: 99%

Ethanol exposure leads to disorder of blood island formation in early chick embryo

Wang

Chen

Wang

et al. 2017

Reproductive Toxicology

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exposure leads to disorder of blood island formation in early chick embryo.Reproductive Toxicology http://dx.doi.org/10. 1016/j.reprotox.2017.08.003 This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. 2,2′-azobis-amidinopropane dihydrochloride, a ROS inducer, which gave a similar anti-vasculogenesis effect as ethanol and this anti-vasculogenesis effect could be reversed by vitamin C. Overall, exposing early chick embryos to ethanol represses blood island progenitor cell migration but disturbed differentiation at a different stage, so that the disorder of blood island formation occurs through excess ROS production and altered vascular-associated gene expression.

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Genetic improvement of xylose metabolism by enhancing the expression of pentose phosphate pathway genes in Saccharomyces cerevisiae IR-2 for high-temperature ethanol production

Cited by 25 publications

References 39 publications

Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics

Improved xylose tolerance and 2,3-butanediol production of Klebsiella pneumoniae by directed evolution of rpoD and the mechanisms revealed by transcriptomics

Second-Generation Bioethanol from Coconut Husk

Ethanol exposure leads to disorder of blood island formation in early chick embryo

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