Genetic Improvement of Rapeseed Mustard through Induced Mutations

Choudhary, Vinod; Jambhulkar, Sanjay J.

doi:10.1016/b978-0-12-801309-0.00016-1

Cited by 4 publications

(1 citation statement)

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“…Malik et al (1999) reported an apetalous line with all six stamens antherless that was obtained from multiple crosses of (Diplotaxis berthautii × B. rapa) × B. juncea; however, the genome configuration of this apetalous line and the molecular mechanism related to the apetalous trait were not further detected. For Brassica crops, apetalous resources have been widely reported in B. napus, which can be obtained from natural mutations (Buzza, 1983;Zhao and Wang, 2004;Zhao et al 2007), physicochemical mutagenesis (Jambhulkar 2007;Zhang et al 2011;Choudhary and Jambhulkar 2016), and intervarietal hybridization (Rao, 1991). Intriguingly, Pierre et al (1996) generated an apetalous B. napus line from reciprocal pair crosses of B. napus and B. carinata.…”

Section: Discussionmentioning

confidence: 99%

Development, identification, and gene expression profiles of an apetalous Brassica juncea derived from interspecific hybridization

Yu,

Feng,

Zhou

et al. 2023

Preprint

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Interspecific hybridization is a powerful approach to broaden genetic diversity and generate novel variations in crops. Apetalous Brassica juncea (AABB, 2n=36) has great interest in breeding programs, but apetalous germplasm is quite rare. In this study, apetalous B. juncea (ABJ) that can stably inherit was obtained by interspecific hybridization between B. juncea and B. carinata (BBCC, 2n=34). Cytological observation showed that the chromosome behaviour of ABJ was normal, with 18 bivalents at diakinesis and chromosome segregation of 18:18 at anaphase І. Moreover, fluorescence in situ hybridization (FISH) using a B genome-specific repeat sequence as a probe showed that the chromosome configuration of ABJ, which comprised 16 B-genome chromosomes and 20 A-genome chromosomes, was consistent with that of the parental B. juncea. However, the combination analysis of RNA-seq and molecular markers eventually demonstrated that chromosome A3 lost a 15.8 Mb fragment in ABJ. Based on the gene expression profile in ABJ, several genes related to petal growth and development were screened out. The apetalous B. juncea was the result of a deleted chromosome fragment was first obtained through interspecific hybridization, which not only provides a novel resource for genetic improvement of apetalous B. juncea but also offers an ideal material for analysing the molecular mechanism of petal development in B. juncea.

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