Abstract:The main aim of this study was to introduce HVA1 gene into Catharanthus roseus callus by using PDS (Particle delivery system). In vitro seed germination of C. roseus was achieved using vigor seeds. The shootlets induction medium was MS containing 2.0 mg/l benzyl aminopurine l −1 . Somatic embryogenesis was obtained by culturing various parts of explants (stem, root and leaves disc) on MS containing 2, 4-D at 3.0 mg/l and subcultured on MS medium containing coconut juice 3%. Genetically transformed C. roseus so… Show more
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