Genetic identification of Anisakis larvae in European hake from Atlantic and Mediterranean waters for stock recognition

Abstract

The occurrence of seven species of the larval parasitic nematode Anisakis, which can be used as a biological tag for hake Merluccius merluccius stocks throughout their geographical range, is reported. Hake were collected from 14 localities in the Mediterranean Sea and the Atlantic Ocean. Anisakis larvae (n = 1950), which were recovered, were identified to species by means of genetic markers (allozymes). Within Anisakis type I, the larvae of A. pegreffii, A. simplex s.s., A. typica and A. ziphidarum were detected, while within Anisakis type II, A. physeteris, A. brevispiculata and Anisakis sp. were identified. There were significant differences in the relative proportions of the various Anisakis species identified in hake samples from the Mediterranean Sea and Atlantic Ocean, suggesting the existence of different stocks of M. merluccius in European waters. © 2004 The Fisheries Society of the British Isles

Changes in size at maturity of European hake Atlantic populations in relation with stock structure and environmental regimes

“…In the light of the present results on the dynamics of Northern and Southern Stocks of hake in recent years it seems that the European hake probably comprises a single population in North Atlantic but has different sub-populations. This hypothesis is supported by genetic and parasitological studies (Lo Brutto et al, 2004;Mattiucci et al, 2004;Castillo et al, 2005) …”

“…However, some controversy exists about the stock structure of European hake in the Atlantic. Some authors consider that there is no separation between traditional Northern and Southern stock because of intense gene flow related to the current systems of this area (Balado et al, 2003) Recent studies based on genetic and biological parameters and incidence of identified parasites used as tags for stock identification suggest that only one stock is really differentiated in the North-east Atlantic; although probably two sub-populations exist and are separated by the Cape Breton Canyon (Lo Brutto et al, 2004;Mattiucci et al, 2004;Castillo et al, 2005). These results are relevant for management purpose and the boundary between these stocks should be reconsidered based on biological evidence.…”

Molecular systematics, phylogeny and ecology of anisakid nematodes of the genusAnisakisDujardin, 1845: an update

“…Data from: Nascetti et al, 1986;Mattiucci et al, 1997;Paggi et al, 1998c;Mattiucci et al, 2004Mattiucci et al, , 2006; and present paper. (our unpublished data) (Fig.…”

Red vent syndrome in wild Atlantic salmon Salmo salar in Scotland is associated with Anisakis simplex sensu stricto (Nematoda: Anisakidae)

“…The parasite has a complex life cycle involving marine mammals (especially cetaceans) as the final host, and fish, squid and planktonic crustaceans as paratenic or intermediate hosts harbouring the larval stages (Smith & Wootten 1978, Mattiucci et al 2004. Humans can also accidentally become infected by eating raw or undercooked seafood or cephalopods that contain the third-stage larvae (L3), which can be invasive, penetrating the host's stomach or intestinal wall and causing gastroenteric manifestations or inducing allergic reactions (Zaffiro et al 2002).…”