Genetic Identification of Hyalodaphnia Species and Interspecific Hybrids

Billiones, Raquel; Brehm, Michaela; Klee, Julia; Schwenk, Klaus

doi:10.1023/b:hydr.0000041615.65087.06

Cited by 33 publications

(59 citation statements)

References 40 publications

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“…Processes following interspecific hybridization, such as backcrossing and occasional gene flow, may significantly influence the accuracy of genetic and morphological determination in hybridizing species (Billiones et al, 2004;Mallet, 2005;Skage et al, 2007). At the phenotypic level, the occurrence of intermediate morphological forms may complicate the taxonomy in such groups (Mallet, 2005 2006; Schwenk et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

“…As later-generation hybrids exhibit a mosaic of parental alleles, an insufficient number of loci may give incomplete information. In the D. longispina species complex, such an underestimation of recombinant classes has been observed in studies applying commonly used molecular methods-ITS-RFLP (Billiones et al, 2004) as well as the analysis of two species-specific allozyme loci (e.g., Seda et al, 2007). The observed inconsistencies among markers have therefore been mostly attributed to their insufficient discriminatory power, unable to reveal backcrossing and introgression (Billiones et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…In studies on hybridizing Daphnia, it is even more crucial, as some mismatch of the markers can be expected (Schwenk & Spaak, 1995). In our previous studies of the D. longispina complex Petrusek et al, 2008b), we showed that unexpected intraspecific variation may substantially influence the interpretation of restriction patterns from ITS-RFLP (Billiones et al, 2004). As no detailed study has directly compared results of various molecular approaches for taxon identification in this species complex so far, the real extent of these discrepancies remains unknown.…”

Section: Introductionmentioning

confidence: 91%

“…However, very weak and poorly visible bands were not considered. We also occasionally used the original ITS-RFLP method designed by Billiones et al (2004) in order to confirm results obtained by the new protocol, especially in populations where results of ITS-RFLP strongly differed from those obtained by other markers (see ''Results'' section).…”

Section: Its-rflpmentioning

confidence: 99%

“…It still remains in use and continues to provide valuable results Keller et al, 2008;Petrusek et al, 2008b). However, alternative methods for taxon identification have been recently developed, for example ITS-RFLP, the restriction fragment length polymorphism (RFLP) of the internal transcribed spacer region (ITS) of nuclear ribosomal DNA (Billiones et al, 2004;Skage et al, 2007). This approach, inspired by successful use in other hybrid systems (e.g., King et al, 2001;Pfenninger et al, 2002), and allowing analysis of ethanol-preserved material, has been considered promising, although some limitations of the original protocol have quickly been identified .…”

Section: Introductionmentioning

confidence: 99%

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Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

et al. 2010

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Daphnia galeata Sars, D. longispina O. F. Müller and D. cucullata Sars (Crustacea: Cladocera) are closely related species which often produce interspecific hybrids in natural populations. Several marker systems are available for taxon determination in this hybridizing complex, but their performance and reliability has not been systematically assessed. We compared results from identifications by three molecular methods. More than 1,200 individuals from 10 localities in the Czech Republic were identified as parental species or hybrids by allozyme electrophoresis and the analysis of the restriction fragment length polymorphism of the internal transcribed spacer (ITS-RFLP); over 440 of them were additionally analyzed and identified by 12 microsatellite loci. Identification by microsatellite markers corresponded well with allozyme analyses. However, consistent discrepancies between ITS-RFLP and other markers were observed in two out of 10 studied localities. Although some marker discrepancies may have been caused by occasional recent introgression, consistent deviations between ITS-RFLP and other markers suggest a long-term maintenance of introgressed alleles. These results warn against its use as a sole identification method in field studies. Additionally, we quantitatively evaluated the discriminatory power of geometric morphometric (elliptic Fourier) analysis of body shapes based on photos of over 1,300 individuals pre-classified by allozyme markers. Furthermore, a randomly selected subset of 240 individuals was independently determined from photos by several experts. Despite a tendency for morphological divergence among parental Daphnia species, some taxa (especially D. galeata, D. longispina, and their hybrids) substantially overlapped in their body shapes. This was reflected in different

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 91%

Section: Its-rflpmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

et al. 2010

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Dynamic reticulate evolution in a Daphnia multispecies complex

Gießler

Englbrecht

2009

J. Exp. Zool.

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Recent genomic data suggest that the role of hybridization in evolution might be more important than previously assumed. Here, we examine species-specific differentiation and signatures of reticulation in a multi-species complex of the crustacean genus Daphnia. We did a combined examination of mtDNA, allozymes and ITS1 (a part of the nuclear ribosomal DNA) in the Daphnia longispina group. We focused on the sequence variation of ITS1 in two unambiguous species (D. galeata, D. cucullata) and two ecotypes hyalina and rosea within the recently revised taxon D. longispina (O.F. Müller). We found two ITS1-types (S, L) and intra-individual and intra-specific polymorphisms. Another ITS1-type (XL) was restricted to the outgroup D. umbra. S was present in all taxa but occurred as only two variants. Surprisingly, D. galeata and D. cucullata, which were well differentiated by mtDNA and allozymes, were virtually indistinguishable with respect to S-ITS1 (S(cg)). The two ecotypes of D. longispina shared the second S-ITS1-variant (S(rh)) and were therefore almost indistinguishable for all types of molecular markers surveyed. The L-type differed between D. galeata and D. longispina samples, but L was absent in D. cucullata. Between hyalina and rosea ecotypes, we found some L-differentiation. Combined data suggest that reticulate evolution enabled the spread of one S-ITS1-variant (S(cg)) beyond species boundaries and that S-introgression was species-specific, despite contemporary hybridization between all species. Our data have implications for phylogenetic as well as phylogeographic surveys. Because of the dynamic impact of gene flow in multi-species complexes, misinterpretations of presumed species-specific data should be considered.

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Characteristics of Orders, Families, and Genera with a List of European Species

Błędzki

Rybak

2016

Freshwater Crustacean Zooplankton of Europe

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Genetic Identification of Hyalodaphnia Species and Interspecific Hybrids

Cited by 33 publications

References 40 publications

Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

Identifying hybridizing taxa within the Daphnia longispina species complex: a comparison of genetic methods and phenotypic approaches

Dynamic reticulate evolution in a Daphnia multispecies complex

Characteristics of Orders, Families, and Genera with a List of European Species

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