Genetic identification of forensically important Calliphoridae in Portugal

Cainé, Laura; Real, Francisco Corte; Lima, Gabriela Narcizo de; Pontes, Lurdes; Abrantes, D.; Pinheiro, M.F.

doi:10.1016/j.ics.2005.09.153

Cited by 8 publications

(11 citation statements)

References 2 publications

(2 reference statements)

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“…1a). This result correlates with studies by Cainé et al (22) who extracted DNA from 141 maggots and successfully amplified both 305-and 519-bp portions of COI for the identification of numerous blow fly species.…”

Section: Coi Amplificationsupporting

confidence: 89%

“…Interestingly, no issues with larval DNA amplification were documented by Cainé et al. . As problems in larval extraction were only encountered in this study with late second‐ and early third‐instar larvae, the success by Cainé et al.…”

Section: Resultsmentioning

confidence: 57%

“…As problems in larval extraction were only encountered in this study with late second‐ and early third‐instar larvae, the success by Cainé et al. may be linked to the age of the immatures that they used, which they did not specify. The crop may not have been entirely dissected from the extract that failed to amplify.…”

Section: Resultsmentioning

confidence: 84%

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DNA Barcoding Identifies all Immature Life Stages of a Forensically Important Flesh Fly (Diptera: Sarcophagidae)

Meiklejohn

Wallman

Dowton

2013

Journal of Forensic Sciences

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Carrion-breeding insects, such as flesh flies (Diptera: Sarcophagidae), can be used as evidence in forensic investigations. Despite their considerable forensic potential, their use has been limited because morphological species identification, at any life stage, is very challenging. This study investigated whether DNA could be extracted and cytochrome oxidase subunit I (COI) barcode sequences obtained for molecular identification of each immature life stage of the forensically important Australian flesh fly, Sarcophaga (Sarcorohdendorfia) impatiens (Walker). Genomic DNA extracts were prepared from all larval instars and puparia. Amplification of the barcoding region was successful from all extracts, but puparia amplicons were weak. All sequences were identified as S. impatiens with 99.95% confidence using the Barcoding of Life Database (BOLD). Importantly, crop removal was necessary to eliminate PCR inhibition for specimens from late second and early third instars. Similar results are expected for immatures of other carrion-breeding species, enhancing the use of evidence from immature flies in forensic investigations.

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Section: Coi Amplificationsupporting

confidence: 89%

Section: Resultsmentioning

confidence: 57%

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DNA Barcoding Identifies all Immature Life Stages of a Forensically Important Flesh Fly (Diptera: Sarcophagidae)

Meiklejohn

Wallman

Dowton

2013

Journal of Forensic Sciences

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“…This last constraint was present in this study, as less than 50% of the specimens, previously identified morphologically, were blasted in BOLD Systems database at species level. Few molecular studies on forensically important insects using COI barcode region have been done in Portugal, except for the most abundant forensic Diptera (Cainé et al 2006(Cainé et al , 2009Ferreira et al 2011;Oliveira et al 2011;Rolo et al 2013). All sequences were added to the BOLD database and are an important contribution to future species determination through molecular methodologies.…”

Section: Molecular Identificationmentioning

confidence: 99%

“…The mitochondrial cytochrome c oxidase subunit I (COI) gene is well established for this purpose, with reference sequences available for a large number of species (Amendt et al 2011). Concerning Portugal, only a few molecular studies involving the abundant Diptera species with forensic interest have been performed (Cainé et al 2006(Cainé et al , 2009Ferreira et al 2011;Oliveira et al 2011;Rolo et al 2013). Thus, there is still a lack of COI barcode sequences available in public databases for some groups, namely several families of Diptera.…”

Section: Introductionmentioning

confidence: 99%