Genetic identification and nucleotide sequence of the DNA polymerase gene of African swine fever virus

Martins, Angélica N.; Ribeiro, Graça; Marques, Maria Isabel; Costa, João

doi:10.1093/nar/22.2.208

Cited by 14 publications

(9 citation statements)

References 52 publications

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“…The present paper now shows that 76.9% of the 39 WSSV structural genes also have this Inr motif (A/TCAC/G/TT) located in the predicted promoter region. This is important because RNA polymerase II usually recognizes either the TATA box, the Inr, or the combination of both (29,36,57). Since the predicted promoter regions of a high proportion of WSSV structural genes include at least one of these features (82.1% have a TATA box, 76.9% have the Inr motif, and 64.1% have both), and since to date no native WSSV RNA polymerase holoenzyme has been identified, these data suggest that WSSV structural genes (the late genes) may also use the RNA polymerase II of the host for transcription.…”

Section: Discussionmentioning

confidence: 99%

Genomic and Proteomic Analysis of Thirty-Nine Structural Proteins of Shrimp White Spot Syndrome Virus

Tsai

Wang²,

Leu

et al. 2004

J Virol

226

164

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White spot syndrome virus (WSSV) virions were purified from the hemolymph of experimentally infected crayfish Procambarus clarkii, and their proteins were separated by 8 to 18% gradient sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE) to give a protein profile. The visible bands were then excised from the gel, and following trypsin digestion of the reduced and alkylated WSSV proteins in the bands, the peptide sequence of each fragment was determined by liquid chromatography-nano-electrospray ionization tandem mass spectrometry (LC-nanoESI-MS/MS) using a quadrupole/time-of-flight mass spectrometer. Comparison of the resulting peptide sequence data against the nonredundant database at the National Center for Biotechnology Information identified 33 WSSV structural genes, 20 of which are reported here for the first time. Since there were six other known WSSV structural proteins that could not be identified from the SDS-PAGE bands, there must therefore be a total of at least 39 (33 ؉ 6) WSSV structural protein genes. Only 61.5% of the WSSV structural genes have a polyadenylation signal, and preliminary analysis by 3 rapid amplification of cDNA ends suggested that some structural protein genes produced mRNA without a poly(A) tail. Microarray analysis showed that gene expression started at 2, 6, 8, 12, 18, 24, and 36 hpi for 7, 1, 4, 12, 9, 5, and 1 of the genes, respectively. Based on similarities in their time course expression patterns, a clustering algorithm was used to group the WSSV structural genes into four clusters. Genes that putatively had common or similar roles in the viral infection cycle tended to appear in the same cluster.

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Section: Discussionmentioning

confidence: 99%

Genomic and Proteomic Analysis of Thirty-Nine Structural Proteins of Shrimp White Spot Syndrome Virus

Tsai

Wang²,

Leu

et al. 2004

J Virol

226

164

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“…Rodriguez et al 1994;Simon-Mateo et al 1995;Sun et al 1995Sun et al , 1996. Other ASFV proteins share sequence similarity to cellular proteins or enzymes, including those involved in aspects of nucleotide metabolism, DNA replication and repair, transcription, and protein modification, and those that likely account for enzymatic activities present in ASFV virions or induced in infected cells (Baylis et al 1992(Baylis et al , 1993aBoursnell et al 1991;Freije et al 1993;Hammond et al 1992;Lu et al 1993;Martin Hernandez and Tabares 1991;Martins et al 1994;Rodriguez et al 1993b;Yanez et al 1993aYanez et al , 1993bYanez et al , 1993c. Several of these proteins appear to be distantly related to homologs identified in poxviruses (Baylis et al 1993b;Boursnell et al 1991;Freije et al 1993;Martin Hernandez and Tabares 1991;Roberts et al 1993;Yanez et al 1993b).…”

Section: The Asfv Genomementioning

confidence: 99%

African Swine Fever Virus

Tulman

Delhon

et al. 2009

Current Topics in Microbiology and Immunology

132

117

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African swine fever virus (ASFV) is a large, intracytoplasmicallyreplicating DNA arbovirus and the sole member of the family Asfarviridae . It is the etiologic agent of a highly lethal hemorrhagic disease of domestic swine and therefore extensively studied to elucidate the structures, genes, and mechanisms affecting viral replication in the host, virus-host interactions, and viral virulence. Increasingly apparent is the complexity with which ASFV replicates and interacts with the host cell during infection. ASFV encodes novel genes

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“…The ORFs from different isolates varied in number and sequence of repeat units within these repeat arrays. Such size variation due to variable numbers of repeated sequences may be a relatively common property of ASFV-encoded proteins since four more protein sequences which contain repeated sequences have been described (Dixon et al, 1994;Rodriguez et al, 1992Martins et al, 1994;Borca et al, 1994).…”

Section: Immune Potentials Of Jl3l Gene Productmentioning

confidence: 99%

African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats

Sun

Jacobs

Smith

et al. 1995

Journal of General Virology

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The African swine fever virus (ASFV)j 13L gene encodes a 177 amino acid protein (19.0 kDa) with a putative transmembrane domain between residues 32 and 52. There is a potential signal peptide cleavage site at residue 54 and several possible motifs for phosphorylation and myristylation. Rabbit antisera raised against a synthetic peptide from the C terminus of the j 13L ORF identified proteins of 25-27 kDa in cells infected with a recombinant vaccinia virus expressing the j 13L ORF, in ASFV-infected cells and in purified extracellular ASF virions. In ASFV-infected cells the jl3L protein was expressed late during infection and exhibited size variation (25-27 kDa) between the different ASFV strains. Nucleotide sequence analysis of the gene in these strains showed that these size differences were due to variation in the number and sequence of tandemly repeated amino acid repeats. Although ASFV-infected animals made antibodies to the jl3L protein, no protection was observed when pigs were vaccinated with a recombinant vaccinia virus expressing the j 13L ORF.

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Genetic identification and nucleotide sequence of the DNA polymerase gene of African swine fever virus

Abstract: ABSTRACT

Cited by 14 publications

References 52 publications

Genomic and Proteomic Analysis of Thirty-Nine Structural Proteins of Shrimp White Spot Syndrome Virus

Genomic and Proteomic Analysis of Thirty-Nine Structural Proteins of Shrimp White Spot Syndrome Virus

African Swine Fever Virus

African swine fever virus gene j13L encodes a 25-27 kDa virion protein with variable numbers of amino acid repeats

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