Genetic identification and comparative study on life history parameters of two strains belonging to Brachionus plicatilis species complex (Rotifera: Monogononta)

Granada, Luana; Lemos, Marivane; Bossier, Peter; Novais, Sara C.

doi:10.1016/j.aqrep.2022.101309

Cited by 1 publication

(5 citation statements)

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“…Also, for B. koreanus , the LC 50 value increased from 142.69 mg L −1 in neonates to 306.07 mg L −1 when exposing adults. In fact, IBA3 present larger body length than MRS10 during the initial developmental stages (Granada et al 2022 ), which can explain its higher tolerance to this stressor.…”

Section: Discussionmentioning

confidence: 99%

“…High salinity had the same effect on strains’ survival, but lower effect in IBA3 behaviour; while MRS10 showed to be more tolerant to hydrogen peroxide at the survival level, but no differences in tolerance were observed in behaviour for this stressor. Although there are no genetic and morphological differences between strains, MRS10 strain starts reproducing at smaller sizes than IBA3 strain, and IBA3 has a longer life span (Granada et al 2022 ). Foley et al ( 2019 ) indicated several studies, including with invertebrates, where lifespan and stress resistance have shown to be positively related, and studies where extended lifespan and antioxidant capacity are strongly associated.…”

Section: Discussionmentioning

confidence: 99%

“…Clone cultures of two strains, MRS10 and IBA, of monogonont rotifer B. koreanus were used in this study. Species identification was confirmed by analysis of 16S rRNA genome sequences (Granada et al 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…Cultures were fed daily with Tetraselmis sp. at a final concentration of 10 5 cells mL −1 (Granada et al 2022 ).…”

Section: Methodsmentioning

confidence: 99%

“…This is particularly relevant for B. plicatilis since, having once been considered a species with morphological variability, more recent taxonomic and genetic studies revealed it is in fact a species complex (Serra and Fontaneto 2017 ). Studies have been conducted to better understand the differences among biotypes, addressing parameters such as growth and reproduction (Gómez et al 1997 ; Granada et al 2022 ; Hagiwara et al 1995 ), morphology (Ciros-Pérez et al 2001 ; Hwang et al 2013 ), lifespan (Gribble et al 2014 ; Kaneko et al 2011 ; Snare et al 2013 ), response to toxicants (Han et al 2023 ; Kang et al 2019 ), and others.…”

Section: Introductionmentioning

confidence: 99%

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Swimming behaviour as an alternative endpoint to assess differences in abiotic stress sensitivities between strains of Brachionus koreanus (Rotifera: Monogononta)

Granada

Lemos

Bossier

et al. 2023

Environ Sci Pollut Res

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Brachionus plicatilis is a cosmopolitan rotifer used as a model organism in several research areas and as live food in aquaculture. Being a species complex, responses to stressors vary even among strains of the same species and, thus, the responses of one species are not representative of the whole complex. This study aimed to address the effects of extreme salinity ranges, and different concentrations of hydrogen peroxide, copper, cadmium, and chloramphenicol, in two strains of B. koreanus (MRS10 and IBA3) from B. plicatilis species complex, by assessing effects on their survival and swimming capacity. Neonates (0–4 h old) were exposed to the stressors in 48 well-microplates, for 24 and 6 h, to evaluate lethal and behavioural effects, respectively. Tested conditions of chloramphenicol did not show any effects on rotifers. The behavioural endpoint showed to be particularly sensitive to assess the effects of high salinity, hydrogen peroxide, and copper sulfate, as swimming capacity impairment was observed for both strains in the lowest concentrations used in lethal tests. Overall, results showed that IBA3 was more tolerant to the majority of stressors, comparing to MRS10, which may be due to differences in physiological characteristics, highlighting the importance of performing multiclonal experiments. Also, swimming capacity inhibition proved to be a good alternative to the classical lethality tests, being sensitive to lower concentrations and with shorter exposure periods.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

“…Cultures were fed daily with Tetraselmis sp. at a final concentration of 10 5 cells mL −1 (Granada et al 2022 ).…”

Section: Methodsmentioning

confidence: 99%