Genetic homogeneity and high shoot proliferation in banana (Musa acuminata Colla) by altering medium thiamine level and sugar type

El-Mahdy, Marwa T.; Youssef, Muhammad

doi:10.1007/s11627-019-10013-7

Cited by 15 publications

(7 citation statements)

References 35 publications

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“…Only three fragments, obtained with the primer OPA-13, were polymorphic; these fragments exhibited insignificant genetic variability between mother and daughter plants, confirming the genetic stability of the sacha inchi plantlets generated through direct in vitro organogenesis from hypocotyls. Similar results were previously obtained in Musa acuminata [ 32 ] and Solanum melongena L.[ 33 ], which were regenerated from hypocotyls with no evidence of polymorphic bands in RAPD analysis. According to Jaccard's coefficient of similarity, the plants regenerated via organogenesis in the present study had 89% genetic similarity to mother hypocotyls (Table S2), indicating that the micropropagation procedure reliably maintained the genetic stability of plant material.…”

Section: Main Textsupporting

confidence: 88%

Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

et al. 2020

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Objective It is necessary to improve biotech platforms based on in vitro cell tissue culture to support sacha inchi ( Plukenetia volubilis L.) research programs and draw on the nutritional value of the high polyunsaturated fatty acid content of its oilseed. Here, we developed a rapid and efficient method for induction and direct in vitro shoot development for this species. Results Shoots were generated from hypocotyl explants. The highest organogenic response was obtained in woody plant medium supplemented with 1 mg/L thidiazuron and 0.5 mg/L zeatin supplemented with L-glutamine, adenine hemisulfate, and L-arginine. Shoots obtained using this medium were transferred and subcultivated with different concentrations of indole-3-butyric acid and 1-naphthylacetic acid for rooting. For the first time, a histological analysis was performed supporting direct organogenic development in this species. The plantlets obtained were transferred ex vitro with a survival percentage of 80%. The genetic stability of the plants recovered was confirmed by randomly amplified polymorphic DNA analysis. All results indicate that it would be possible to stimulate direct shoot formation from hypocotyls to support the sustainable use of this species.

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confidence: 88%

Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

et al. 2020

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“…Therefore, the genetic homogeneity assessment of in vitro propagated plantlets is of great importance. Genetic fidelity of regenerants is evaluated through a lot of DNA-based molecular markers like Random Amplified Polymorphic DNA (RAPD), Simple Sequence Repeats (SSR), Inter Simple Sequence Repeat (ISSR) and Amplified Fragment Length Polymorphism (AFLP) 30 , 31 . RAPD and ISSR are predominant among the various markers for their high reproducibility, reliability, simplicity and cost-effectiveness 32 .…”

Section: Introductionmentioning

confidence: 99%

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Sultana

Das

Chandra

et al. 2022

Sci Rep

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Thunbergia coccinea Wall. ex D. Don being a rare, ornamental and medicinal plant of India, is needed to propagate for conserving the germplasm and analyzing its phytochemical compounds in the future. A reliable protocol for direct in vitro propagation using nodal shoot meristem of T. coccinea as explant was standardized. The highest number of shoots per explant (22.17 ± 0.54) with maximum shoot length (2.36 ± 0.28) in cm was obtained in Murashige and Skoog (MS) medium supplemented with 9.70 µM of 6-furfurylaminopurine (Kinetin) and 0.053 µM of α-naphthaleneacetic acid (NAA) combination, among all the different plant growth regulators (PGR’s) and concentrations tested. The aforesaid PGR’s combination was optimum for axillary shoot bud induction and multiplication in T. coccinea. The best rooting was observed on the half-strength MS medium fortified with 2.68 µM NAA with the highest number of roots per shoot (3.75 ± 0.12) and maximum length (5.22 ± 0.32) in cm. All the in vitro raised plantlets were acclimatized in sterile sand and soil mixture (1:1) with a survival rate of 70% on earthen pots under greenhouse conditions. PCR-based RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeat) molecular markers were employed to determine the genetic homogeneity amongst the plantlets. Twelve (12) RAPD and nine (9) ISSR primers developed a total of 104 and 91 scorable bands, respectively. The band profiles of micropropagated plantlets were monomorphic to the mother, donor in vivo plant, and similarity values varied from 0.9542–1.000. The dendrogram generated through UPGMA (unweighted pair group method with arithmetic mean) showed 99% similarities amongst all tested plants confirming the genetic uniformity of in vitro raised plants.

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“…Therefore, evaluation of genetic uniformity of in vitro raised plantlets is compulsory. At the present time, quite a lot of DNA based molecular markers for example Random Ampli ed Polymorphic DNA (RAPD), Simple Sequence Repeats (SSR), Inter-Simple Sequence Repeat (ISSR) and Ampli ed Fragment Length Polymorphism (AFLP), have been employed to evaluate the genetic stability of a regenerants (Singh et al 2013;Ebrahimi et al 2018;El-Mahdy and Youssef 2019;Saeed et al 2019). Among the various genetic markers, RAPD and ISSR have been predominantly ideal because of their reproducible, reliability, simplicity and cost-effectiveness (Mehrotra et al 2012).…”

Section: Main Textmentioning

confidence: 99%

Efficient in vitro plant regeneration from leaf-derived callus and genetic fidelity assessment of an endemic medicinal plant Ranunculus wallichianus Wight & Arnn by using RAPD and ISSR markers

Srinivasan

Raja

Tamilvanan

2021

Plant Cell Tiss Organ Cult

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Ranunculus wallichianus is a medicinally important plant and an endemic species to Western Ghats of South India. An e cient and reliable indirect regeneration protocol system for R. wallichianus was developed from leaf explants in the present investigation. Leaf explants were cultured on both fullstrength and half-strength MS (Murashige & Skoog) medium supplemented with different concentrations (1.0 mg L − 1 to 3.0 mg L − 1 ) of 2,4-D and NAA. Among the different concentrations tested, the highest percentage of yellowish green compact nodular callus formation was observed on half-strength MS medium with 2.0 mg L − 1 of 2, 4-D. Then, the in vitro raised organogenic callus was cultured on half strength MS medium containing various concentrations (1.0 mg L − 1 to 3.0 mg L − 1 ) of BA, KIN and TDZ with 0.5 mg L − 1 NAA and 10% CW for in vitro shoot regeneration. The highest percentage of regeneration response (97%) and maximum number of shoots formation (11.1 ± 0.13 shoots/culture with 9.2 ± 0.35 cm mean shoot length) were obtained from MS medium containing 2.5 mg L − 1 BA with 0.5 mg L − 1 NAA and 10% CW. The well elongated in vitro raised shoots were rooted in half strength MS medium with 2.5 mg L − 1 IBA + 250 mg L − 1 activated charcoal shows high frequency of root formation. The well rooted plantlets were successfully hardened and acclimatized with the survival rate of 94%. Clonal delity of in vitro raised plantlets was assessed by using DNA based RAPD and ISSR molecular markers. The total of 56 and 47 monomorphic bands were obtained from RAPD and ISSR markers respectively. This present in vitro propagation protocol system could be an effective for the conservation of R. wallichianus with their genetic purity and its further investigations.

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Genetic homogeneity and high shoot proliferation in banana (Musa acuminata Colla) by altering medium thiamine level and sugar type

Cited by 15 publications

References 35 publications

Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

Efficient direct shoot organogenesis and genetic stability in micropropagated sacha inchi (Plukenetia volubilis L.)

Efficient micropropagation of Thunbergia coccinea Wall. and genetic homogeneity assessment through RAPD and ISSR markers

Efficient in vitro plant regeneration from leaf-derived callus and genetic fidelity assessment of an endemic medicinal plant Ranunculus wallichianus Wight & Arnn by using RAPD and ISSR markers

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