Genetic heterogeneity of the V3 region of the HIV-1 envelope glycoprotein in Brazil

Ke, Potts; Ml, Kalish; Lott, Timothy J.; Orloff, Gregg M.; Cc, Luo; Ma, Bernard; Cb, Alves; Badaro, Roberto; Suleiman, Jamal; Ferreira, Orlando C.

doi:10.1097/00002030-199309000-00007

Cited by 91 publications

(59 citation statements)

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“…Until 1995, 91% (180/199 samples) of identified HIV-1 clades in Brazil 13 were genetically related to the subtype B isolates described in the United States and Haiti; however, 40% of these isolates presented differences from the original B: instead of GPGR, the GWGR motif was found in the crown of V 3 loops, characterizing a Brazilian subtype B. Subtypes F and C comprised 9% of the samples (7 and 2%, respectively). It is worth underscoring that 30% (3 out of 10) of samples isolated from the south of the Country were classified as subtype C. A single B/F recombinant was also detected 9 12 .…”

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Molecular epidemiology of HIV-1 in Rio Grande, RS, Brazil

Martínez

Barbosa

Ferreira

et al. 2002

Rev. Soc. Bras. Med. Trop.

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We conducted a molecular epidemiological study to investigate HIV-1 strains in Rio Grande, southern Brazil, searching for an association with transmission mode and risk behavior. Patients (185) identified at an AIDS treatment reference Hospital, from 1994 to 1997, were included; from which 107 blood samples were obtained. Nested PCR was realized once for each sample; for amplified samples (69) HIV subtypes were classified using the heteroduplex mobility assay. Subtypes identified were B (75%), C (22%) and F (3%). All infections with C were diagnosed after 1994. Comparing patients with B and C, no differences were detected regarding demographic, clinical and laboratory characteristics; survival analysis did not reveal differences in HIV to AIDS evolution. A higher proportion of injecting drug users, IDU (not significant, p<.07) was found among those with C. This suggests that C may have been introduced in this area through IDU, and is being spread, probably by their sexual partners, to persons with other risk practices.

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confidence: 99%

Molecular epidemiology of HIV-1 in Rio Grande, RS, Brazil

Martínez

Barbosa

Ferreira

et al. 2002

Rev. Soc. Bras. Med. Trop.

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“…There are at least four different HIV-1 subtypes circulating in Brazil (B, F, C, D), and subtype B predominates (4)(5)(6)(7)(8)(9)(10). Recently, it was reported that two genetically and antigenically distinct strains of HIV-1 subtype B cocirculate in Brazil.…”

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Two variants of HIV-1 B serotype are transmitted heterosexually in São Paulo, Brazil

Casseb

Hong

Gonsalez

et al. 1998

Braz J Med Biol Res

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HIV-1 variability may have an important impact on transmission and pathogenicity. Better characterization of the HIV epidemic in Brazil is necessary for the development of vaccine trials in this country. We analyzed sera from 108 HIV-1-infected volunteers from São Paulo City to determine serotype and reactivity for V3 motifs of HIV in this population, and the relationship to transmission mode. We concluded that the HIV-1 B serotype is frequent among heterosexually infected women, even in the absence of anal sex, and that two major V3 motifs, GPGR and GWGR, had similar prevalence among women (48% and 52%, respectively) and men (56% and 44%, respectively). We also observed an equal distribution of these strains regardless of their CD4+ T cell counts, clinical status, and mode of transmission. Even though V3 serology for HIV-1 subtyping is an inexpensive tool for use in developing countries, additional methods, such as heteroduplex mobility assay and direct DNA sequencing, should be included to determine HIV-1 genetic diversity.

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“…PCR was performed on PBMC DNA. [Potts et al(1993b)]. Accession numbers for 21 of the 56 clones from which consensus sequences were calculated: L19225-L19237, L19240-L19246 and L20963.…”

Section: M-140 Dec 97mentioning

confidence: 99%

“…Subtypes of all 24 patients were also tested by HMA. [Campodonico et al(1996) [Potts et al(1993b)]. Accession number L19237.…”

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confidence: 99%