Genetic heterogeneity of Campylobacter jejuni NCTC 11168 upon human infection

Revez, Joana; Schott, Thomas; Llarena, Ann-Katrin; Rossi, Mirko; Hänninen, Marja‐Liisa

doi:10.1016/j.meegid.2013.03.009

Cited by 22 publications

(40 citation statements)

References 18 publications

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“…Our findings are concordant with the results of other studies performed on C. jejuni isolates from infected patients and analyzed over a short period (11,13). For example, Cody et al (11) showed that two C. jejuni isolates from a patient that were cultivated in the morning and evening on the same day had differences in six loci.…”

Section: Discussionsupporting

confidence: 92%

“…For example, homologs of the loci CAMP1214, CAMP1242, CAMP1243, and CAMP1258 are located in NCTC 11168 in the flagella region. In our previous study on accidental C. jejuni infection, we also found homopolymeric tract changes in some of these loci after passage through a human patient (13). The homolog of CAMP1331 (Cj1420) is a methyltransferase located in the capsule locus.…”

Section: Discussionmentioning

confidence: 90%

“…The genome sequence of C. jejuni strain Ma_1 was obtained using Illumina sequencing technology with 100 cycles of paired-end reads, whereas the genomic sequences of the five remaining isolates and the strain 1_12S were obtained by Illumina HiSeq single-end reads. The assembly and annotation were performed as previously described (13). Briefly, the Illumina reads were trimmed using the ConDeTri Perl script (14) with the default settings and a minimum read length of 75 nucleotides.…”

Section: Methodsmentioning

confidence: 99%

“…This approach was extensively applied, e.g., during the large multistate Escherichia coli O104:H4 epidemic (12). WGS will change the way we compare isolates because it allows for the analysis of all potential diversities and enables the detection of novelties associated with genome synteny, sequences, single genes, and polymorphisms between the isolates, as well as potential changes in the genomes that occur during infection (10,13). We recently found that only a few single-nucleotide polymorphisms (SNPs) are detected in C. jejuni after its intestinal passage through an infected patient (13).…”

mentioning

confidence: 99%

“…WGS will change the way we compare isolates because it allows for the analysis of all potential diversities and enables the detection of novelties associated with genome synteny, sequences, single genes, and polymorphisms between the isolates, as well as potential changes in the genomes that occur during infection (10,13). We recently found that only a few single-nucleotide polymorphisms (SNPs) are detected in C. jejuni after its intestinal passage through an infected patient (13). However, there is limited knowledge regarding how to interpret the results of WGS and comparative genomics of outbreak-associated C. jejuni isolates and how to combine them with other epidemiological data (i.e., differences in SNPs and homopolymeric tracts in contingency genes).…”

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confidence: 99%

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Genomic Variation between Campylobacter jejuni Isolates Associated with Milk-Borne-Disease Outbreaks

et al. 2014

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bBacterial genome sequencing has led to the development of new approaches for the analysis of food-borne epidemics and the exploration of the relatedness of outbreak-associated isolates and their separation from nonassociated isolates. Using Illumina technology, we sequenced a total of six isolates (two from patients, two from raw bulk milk, and two from dairy cattle) associated with a milk-borne Campylobacter jejuni outbreak in a farming family and compared their genomes. These isolates had identical pulsed-field gel electrophoresis (PFGE) types, and their multilocus sequence typing (MLST) type was ST-50. We used the Ma_1 isolate (milk) as the reference, and its genome was assembled and tentatively ordered using the C. jejuni NCTC 11168 genome as the scaffold. Using whole-genome MLST (wgMLST), we identified a total of three single-nucleotide polymorphisms (SNPs) and differences in poly(G or C) or poly(A or T) tracts in 12 loci among the isolates. Several new alleles not present in the database were detected. In contrast, the sequences of the unassociated C. jejuni strains P14 and 1-12S (both ST-50) differed by 420 to 454 alleles from the epidemic-associated isolates. We found that the fecal contamination of bulk tank milk occurred by highly related sequence variants of C. jejuni, which are reflected as SNPs and differences in the length of the poly(A or T) tracts. Poly(G or C) tracts are reversibly variable and are thus unstable markers for comparison. Further, unrelated strains of ST-50 were clearly separated from the outbreak-associated isolates, indicating that wgMLST is an excellent tool for analysis. In addition, other useful data related to the genes and genetic systems of the isolates were obtained.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 90%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Genomic Variation between Campylobacter jejuni Isolates Associated with Milk-Borne-Disease Outbreaks

et al. 2014

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Multilocus Sequence Types of Campylobacter jejuni Isolates from Different Sources in Eastern China

Zhang

et al. 2015

Curr Microbiol

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Campylobacter jejuni is a major food-borne pathogen that causes human gastroenteritis in many developed countries. In our study, we applied multilocus sequence typing (MLST) technology to 167 C. jejuni isolates from diverse sources in Eastern China to examine their genetic diversity. MLST defined 94 sequence types (STs) belonging to 18 clonal complexes (CCs). Forty-five STs from 60 isolates (36%) and 22 alleles have not been previously documented in an international database. One hundred and two isolates, accounting for 61.1% of all isolates, belonged to eight clonal complexes. The eight major CCs were also the most common complexes from different sources. The most common ST type of isolates from human and food was ST-353. The dominant ST type in chicken and foods was ST-354. Among 21 STs that contained two or more different sources isolates, 15 STs contained human isolates and isolates from other sources, suggesting that potentially pathogenic strains are not restricted to specific lineages.

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Tracing isolates from domestic human Campylobacter jejuni infections to chicken slaughter batches and swimming water using whole-genome multilocus sequence typing

Kovanen

Kivistö

Llarena

et al. 2016

International Journal of Food Microbiology

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Campylobacter jejuni is the leading cause of bacterial gastroenteritis and chicken is considered a major reservoir and source of human campylobacteriosis. In this study, we investigated temporally related Finnish human (n=95), chicken (n=83) and swimming water (n=20) C. jejuni isolates collected during the seasonal peak in 2012 using multilocus sequence typing (MLST) and whole-genome MLST (wgMLST). Our objective was to trace domestic human C. jejuni infections to C. jejuni isolates from chicken slaughter batches and swimming water. At MLST level, 79% of the sequence types (STs) of the human isolates overlapped with chicken STs suggesting chicken as an important reservoir. Four STs, the ST-45, ST-230, ST-267 and ST-677, covered 75% of the human and 64% of the chicken isolates. In addition, 50% of the swimming water isolates comprised ST-45, ST-230 and ST-677. Further wgMLST analysis of the isolates within STs, accounting their temporal relationship, revealed that 22 of the human isolates (24%) were traceable back to C. jejuni positive chicken slaughter batches. None of the human isolates were traced back to swimming water, which was rather sporadically sampled. The highly discriminatory wgMLST, together with the patient background information and temporal relationship data with possible sources, offers a new, accurate approach to trace back the origin of domestic campylobacteriosis. Our results suggest that potentially a substantial proportion of campylobacteriosis cases during the seasonal peak most probably are due to other sources than chicken meat consumption. These findings warrant further wgMLST-based studies to reassess the role of other reservoirs in the Campylobacter epidemiology both in Finland and elsewhere.

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Genetic heterogeneity of Campylobacter jejuni NCTC 11168 upon human infection

Cited by 22 publications

References 18 publications

Genomic Variation between Campylobacter jejuni Isolates Associated with Milk-Borne-Disease Outbreaks

Genomic Variation between Campylobacter jejuni Isolates Associated with Milk-Borne-Disease Outbreaks

Multilocus Sequence Types of Campylobacter jejuni Isolates from Different Sources in Eastern China

Tracing isolates from domestic human Campylobacter jejuni infections to chicken slaughter batches and swimming water using whole-genome multilocus sequence typing

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