Genetic heterogeneity and subtyping of human Hepatitis E virus isolates from Uruguay

Mirazo, Santiago; Ramos, Natália; Russi, José; Arbiza, Juan

doi:10.1016/j.virusres.2013.01.005

Cited by 34 publications

(36 citation statements)

References 49 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The KJ701409 genome obtained here shared 84.2 to 88.1% identity with HEV 3i genomes FJ705359 and FJ998008, which is greater than the lower limit of identity at the level of subtype defined by Lu et al for HEV 3/4 genomes (82.0 to 87.9%) (3), and 82.8% identity with genome JQ013794, labeled as 3c; identity with the EU360977 HEV genome, also labeled as 3c, was 78.8%. Finally, we analyzed 285-nucleotide-long fragments from ORF2 (corresponding to nucleotides 6000 to 6284 of genome FJ705359) that were described in Uruguay as belonging to subtype 3i (17) and observed that these sequences were clustered with the HEV 3i genomes (mean Ϯ SD identity, 88.8% Ϯ 0.3%) and with HEV sequences recovered from our four kidney transplant recipients (86.5% Ϯ 1.3%) (data not shown).…”

Section: Resultsmentioning

confidence: 95%

“…Using short HEV ORF2 fragments may therefore generate misclassification. HEV 3i has been identified in five countries (Austria, Argentina, Bolivia, Germany, and Uruguay) and from various hosts, including humans (3,14,17), wild boars (12,13), and swine (11,17). HEV sequences from strains wbGER27 (FJ705359) and BB02 (FJ998008), which were recovered from wild boars in Germany, were fully sequenced and considered the first HEV 3i genomes (12,13).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Hepatitis E Virus of Subtype 3i in Chronically Infected Kidney Transplant Recipients in Southeastern France

et al. 2014

View full text Add to dashboard Cite

e Hepatitis E virus (HEV) is a leading cause of waterborne acute hepatitis in developing countries. In Europe, HEV causes a zoonotic disease and is hyperendemic in southern France. Four HEV genotypes (1 to 4) have been defined, and the most used classification divides them into 24 subtypes. Autochthonous European HEV strains belong in majority to genotype 3. Subtypes 3c, 3f, and 3e are representative of the HEV diversity in France. HEV causes chronic hepatitis in solid-organ transplant recipients in Europe, and viral characteristics associated with chronicity are poorly documented. We sequenced 343-nucleotide-long HEV genomic fragments from the serum of eight chronically infected kidney transplant recipients and a near-full-length genome in one case. We identified in four patients (50%) HEV of subtype 3i, not described previously in France. If shorter genomic fragments were used in phylogenetic analyses, these HEV sequences were clustered with open reading frame 2 (ORF2) fragments labeled as subtype 3c. At least five of the eight HEV 3i sequences recovered from humans in our phylogenetic analyses were from chronically infected kidney transplant recipients. These data show that the description of the prevalence and geographical distribution of HEV subtypes may be partially inaccurate and that criteria for classification as 3i and 3c should be clarified. Extended molecular virology analyses are required to improve knowledge of HEV epidemiology and determinants of chronic HEV infection.

show abstract

Section: Resultsmentioning

confidence: 95%

Section: Discussionmentioning

confidence: 99%

Hepatitis E Virus of Subtype 3i in Chronically Infected Kidney Transplant Recipients in Southeastern France

et al. 2014

View full text Add to dashboard Cite

show abstract

“…This inconsistency has been previously observed associated with important differences in the performance of serological and PCR assays [19,20]. In addition, noteworthy differences in the sensitivity and efficiency of detection have been observed among different HEV genome regions [21].…”

Section: Discussionmentioning

confidence: 91%

Hepatitis E Virus Genotype 1 and Hepatitis A Virus Dual Infection in Pediatric Patients with a Low Socioeconomic Status from Mexico

et al. 2018

Self Cite

View full text Add to dashboard Cite

Objective: We aimed to detect and characterize hepatitis E virus (HEV) RNA in sera samples from a pediatric population infected with the hepatitis A virus (HAV) exhibiting acute hepatitis and to correlate the infection status with the clinical outcome. Methods: Seventy-five ELISA-positive samples from children containing anti-HAV and anti-HEV IgM were used to amplify and characterize partial regions within HEV ORF2. A statistical comparison of clinical data between HEV IgM-positive/HEV RNA-positive patients and HEV IgM-positive/HEV RNA-negative patients was performed. Results: Thirteen out of 75 IgM-positive samples provided amplification of discrete regions of the HEV genome. Nested RT-PCR-based detection and subsequent sequencing of 5 samples confirmed the identity of HEV genotype 1 (G1), which had not been previously reported in Mexico. Though not significant, a trend towards exacerbated clinical manifestations was found in HEV RNA-positive patients relative to HEV RNA-negative patients. Conclusions: An elevated rate of G1 RNA was detected. Hepatitis E seems to be a neglected disease in Mexico and epidemic strains of HEV are likely to play a role as causative agents of acute hepatitis in highly exposed children. Although HAV is endemic in Mexico, an HEV-RNA detection rate of 17% in co-infected samples shows the need for screening for HEV as a part of future vaccination strategies.

show abstract

“…Since then, the number of cases has increased. Molecular epidemiology of HEV suggests that HEV-G3 is most prevalent among the human population, but epidemic HEV-G1 strains have also been reported (17,18). Phylodynamic and evolutionary studies on HEV-G3 isolates have shown that HEV infection in Uruguay is an emergent process.…”

Section: Hev In the Environment Humans And Swine In Uruguaymentioning

confidence: 99%

“…HEV-G4 is almost exclusively restricted to Asia (3,4,7). HEV genotypes and reservoirs from nine LAC countries (Argentina, Bolivia, Brazil, Colombia, Costa Rica, Cuba, Mexico, Uruguay, Venezuela) (8)(9)(10)(11)(12)(13)(14)(15)(16)(17)(18)(19) are shown in Table 1. Specific clinical manifestations of HEV infection have been tentatively associated with distinct genotypes; for example, HEV-G1 is related to fulminant acute hepatitis in pregnancy, whereas HEV-G3 is associated with chronic liver disease in immunosuppressed patients (2,7).…”

mentioning

confidence: 99%

Challenges in research and management of hepatitis E virus infection in Cuba, Mexico, and Uruguay

Realpe

Montalvo

Mirazo

et al. 2018

Rev Panam Salud Publica

Self Cite

View full text Add to dashboard Cite

Genetic heterogeneity and subtyping of human Hepatitis E virus isolates from Uruguay

Cited by 34 publications

References 49 publications

Hepatitis E Virus of Subtype 3i in Chronically Infected Kidney Transplant Recipients in Southeastern France

Hepatitis E Virus of Subtype 3i in Chronically Infected Kidney Transplant Recipients in Southeastern France

Hepatitis E Virus Genotype 1 and Hepatitis A Virus Dual Infection in Pediatric Patients with a Low Socioeconomic Status from Mexico

Challenges in research and management of hepatitis E virus infection in Cuba, Mexico, and Uruguay

Contact Info

Product

Resources

About