Genetic fine‐mapping of anthracnose disease‐resistance allele Co‐1⁴ present in the Andean common bean cultivar AND 277

Abstract

Anthracnose (ANT) is among the common bean fungal diseases responsible for significant yield and grain quality losses. Durable genetic resistance is the primary ANT control method due to the pathogen's high variability. Genetic studies showed that the common bean chromosome Pv01 contains multiple disease resistance genes, includ- and CoPv01 CDRK . This work aimed to: (i) perform the genetic fine-mapping of the Co-1 4 allele present in the cultivar AND 277, using recombinant inbred lines derived from the cross between Rudá × AND 277; and (ii) identify candidate resistance genes in the Co-1 4 allele based on the common bean reference genome. Initially, the Co-1 4 allele was mapped between the single-nucleotide polymorphism markers ss715645251 and ss715645250 at a distance of 2.0 and 19.6 cM, respectively. Fine-mapping localized Co-1 4 between the markers ss715645251 and BARCPVSSR01356, spanning a 40.51 kb region at the end of Pv01. Two genes are described within the Co-1 4 region in the reference genome, Phvul.001G243800 and Phvul.001G243900. The linkage between the Co-1 4 allele and the markers ss715645251 and BARCPVSSR01356 will be essential for plant breeding programs during the resistance gene transfer to elite cultivars via markerassisted selection (MAS). Identifying and functionally analyzing candidate resistance genes in this region will allow more efficient MAS by developing accurate markers for ANT resistance.

Genome-wide association analysis of anthracnose resistance in the Yellow Bean Collection of Common Bean

“…The SNPs most significantly associated with resistance to races 81, 1050, and 1105 were found at positions 49,584,097, 49,583,965, and 49,584,097 of Pv01, respectively. In each case, the most SNP most significantly associated with ANTH resistance is in the gene model Phvul.001G243800, a protein-kinase recently proposed to underlie Co-1 [37,38]. This is a powerful validation of the role of this gene, as well as the experimental methods employed in this study.…”

Responsiveness of Candidate Genes on CoPv01CDRK/PhgPv01CDRK Loci in Common Bean Challenged by Anthracnose and Angular Leaf Spot Pathogens

“…Although several independent genes confer resistance to C. lindemuthianum, most resistance genes found in Andean cultivars have been mapped to the common bean chromosome Pv01. The Co-1, Co-1 2 , Co-1 3 , Co-1 4 , Co-1 5 and Co-1 HY alleles of the Co-1 2 genes are present in the cultivars Michigan Dark Red Kidney (MDRK), Kaboon, Perry Marrow, AND 277, Widusa and Hongyundou, respectively [9][10][11][12]. Other resistance genes were mapped to the end of Pv01: Co-x in Jalo EEP558, Co-AC i n A m e n d o i m C a v a l o [ 1 3 , 1 4 ] .…”

Whole-genome resequencing of common bean elite breeding lines

“…By integrating genomic knowledge, such as genomic structure, function, diversity, with quantitative genetics, a solid foundation has been established for detailed dissection of the genomic architecture of genetic variation 13 – 15 . In addition, this integration has provided valuable insights into the evolutionary and domestication history of plants, as well as the genetic mechanisms of adaptations and selective breeding that have contributed to the environment’s adaptation 16 .…”