Genetic Features of the Widespread Plasmid Coding for the Carbapenemase OXA-48

Poirel, Laurent; Bonnin, Rémy A.; Nordmann, Patrice

doi:10.1128/aac.05289-11

Cited by 340 publications

(334 citation statements)

References 27 publications

Supporting

Mentioning

303

Contrasting

Unclassified

Order By: Relevance

“…This enzyme was firstly reported from a K. pneumoniae isolate in Turkey in 2001 (22). Later, it was reported from different hospitals with increased rates (29,32).…”

Section: Discussionmentioning

confidence: 99%

“…All carbapenem-resistant K. pneumoniae isolates were tested for the presence of carbapenemase-encoding genes including blaOXA-48, blaNDM-1, blaKPC, blaIMP, and blaVIM by the multiplex PCR using the primers described previously (20)(21)(22). A bacterial suspension (equal to McFarland 4 turbidity) was prepared in sterile water and boiled for 10 minutes.…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

“…The PCR reaction mixture contained 1. conditions were used: initial denaturation at 94°C for 3 minutes, followed by 35 cycles of 94°C for 30 seconds, 55°C for 30 seconds, and 72°C for 1 minute, with a final extension step at 72°C for 7 minutes. The amplified DNA products were analyzed by electrophoresis on a 1.5% agarose gel and the results were evaluated according to the size of each amplicon (20)(21)(22).…”

Section: Polymerase Chain Reactionmentioning

confidence: 99%

See 2 more Smart Citations

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Çelikbilek¹,

Ünaldı²,

Kırca³

et al. 2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

Background: Carbapenem-resistant Klebsiella pneumoniae isolates can spread among the hospitalized patients and result in serious infections and increase mortality. Molecular studies provide useful information about resistance mechanisms and crosstransmission among the resistant isolates. Objectives: The current study aimed at investigating phenotypic and molecular characteristics of the carbapenem resistance and clonal relationship among the K. pneumoniae isolates recovered from infection sites and rectal swabs of the patients hospitalized in a tertiary hospital. Methods: Resistance to carbapenems was investigated by disc-diffusion and E-test methods. Modified Hodge test (MHT) and ethylenediaminetetraacetic acid (EDTA)-combine disc (ECD) methods were performed to determine carbapenemases. Carbapenemase-encoding genes including blaOXA-48, blaNDM-1, blaKPC, blaIMP, and blaVIM were investigated by multiplex polymerase chain reaction (PCR). Clonal relationship among the isolates was determined by the pulsed-field gel electrophoresis (PFGE). Results: Carbapenem resistance was observed in 93 (5.8%) of the 1605 K. pneumoniae isolates. Majority (n = 66, 71%) of the resistant isolates were recovered from the patients in intensive care units (ICUs). Almost all carbapenem-resistant K. pneumoniae isolates (n = 91, 97.8%) were positive with MHT. Only 6 (6.5%) of the resistant isolates were positive with ECD method. The blaOXA-48 and blaNDM-1 were found in 90.3% (n = 84) and 6.5% (n = 6) of the resistant isolates, respectively. The pulsed-field gel electrophoresis yielded

show abstract

“…This enzyme was firstly reported from a K. pneumoniae isolate in Turkey in 2001 (22). Later, it was reported from different hospitals with increased rates (29,32).…”

Section: Discussionmentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

Section: Polymerase Chain Reactionmentioning

confidence: 99%

See 1 more Smart Citation

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Çelikbilek¹,

Ünaldı²,

Kırca³

et al. 2017

Jundishapur J Microbiol

View full text Add to dashboard Cite

show abstract

“…Successful horizontal transfer of OXA-48-encoding plasmids within patients had been reported previously in the UK and France [14,15]. In addition, Berger et al showed that the mobilization of the OXA-48 gene is not restricted to the transfer of a single plasmid, but may also be involved in multiple rearrangements from different plasmids originating from different Enterobacteriaceae species [16].…”

mentioning

confidence: 85%

Retrospective identification of a previously undetected clinical case of OXA-48-producing K. pneumoniae and E. coli: the importance of adequate detection guidelines

et al. 2015

View full text Add to dashboard Cite

Escherichia coli (ST866) in the Netherlands. A similar IncL/M plasmid was identified in both strains, suggesting within-patient horizontal transfer. Conclusion This case illustrates that OXA-48-carbapenemase-producing Enterobacteriaceae can be unnoticed without adequate laboratory detection procedures. Our observation stresses the importance of uniform and adequate laboratory methods for the timely and accurate detection of important antimicrobial resistance.

show abstract

“…The bla OXA-48 gene has been almost always located on a conjugative plasmid of ca. 62 kb assigned to the IncL/M group and has been identified only in enterobacterial species [5,6]. Both CTX-M-15 and OXA-48 have been detected in several enterobacterial species, being particularly frequent in Klebsiella pneumoniae, whilst they are relatively uncommon in E. cloacae [2,3].…”

Section: Introductionmentioning

confidence: 99%

Dissemination of multiresistant Enterobacter cloacae isolates producing OXA-48 and CTX-M-15 in a Spanish hospital

Fernández

Montero

Martinez

et al. 2015

International Journal of Antimicrobial Agents

Self Cite

View full text Add to dashboard Cite

Twenty-one multiresistant Enterobacter cloacae isolates producing OXA-48 (n = 10), CTX-M-15 (n = 7) or both (n = 4) ␤-lactamases were detected in a Spanish hospital during a 1-year period (June 2013 to June 2014). The isolates were also resistant to non-␤-lactam antimicrobials, further complicating the therapeutic options. Genotyping of the isolates identified two major clones (ST74 and ST66) that caused prolonged outbreaks in different buildings of the hospital as well as some sporadic isolates (ST78, ST45 and ST295). Isolates belonging to clone 1 (n = 7) were carbapenem-resistant and carried the bla OXA-48 gene on a conjugative IncL/M plasmid of ca. 65 kb. Clone 2 isolates (n = 11) were resistant to cefepime and harboured the bla CTX-M-15 gene on an ca. 150-kb, non-conjugative plasmid of the IncF group, co-harbouring the qnrB and aac(6 )-Ib-cr genes encoding quinolone resistance. Four clone 2 isolates were also resistant to carbapenems owing to the co-production of OXA-48. Most of the isolates were recovered from critically ill patients and were admitted to intensive care units; a single patient was transferred from another Spanish hospital. Intrahospital and interhospital dissemination of multiresistant E. cloacae isolates is of major clinical concern as it could lead to endemic nosocomial situations.

show abstract

Genetic Features of the Widespread Plasmid Coding for the Carbapenemase OXA-48

Cited by 340 publications

References 27 publications

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Molecular Characterization of Carbapenem-Resistant Klebsiella pneumoniae Species Isolated From a Tertiary Hospital, Ankara, Turkey

Retrospective identification of a previously undetected clinical case of OXA-48-producing K. pneumoniae and E. coli: the importance of adequate detection guidelines

Dissemination of multiresistant Enterobacter cloacae isolates producing OXA-48 and CTX-M-15 in a Spanish hospital

Contact Info

Product

Resources

About