Genetic features of highly pathogenic avian influenza viruses A(H5N8), isolated from the European part of the Russian Federation

Voronina, Olga L.; Ryzhova, N. N.; Aksenova, Ekaterina I.; Kunda, Marina S.; Sharapova, N. E.; Fedyakina, I. Т.; Chvala, Ilya; Borisevich, S. V.; Logunov, Denis Y.; Gintsburg, A. L.

doi:10.1016/j.meegid.2018.05.022

Cited by 10 publications

(6 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…By 4 weeks PV, the anti-HPAI H5N8 antibody titers were ≥5.0 log2. These relatively low antibody titers were rather expected due to the genetic and antigenic differences in the HA between HPAI H5N1 and H5N8 viruses isolated in Egypt [25,26].…”

Section: Discussionmentioning

confidence: 99%

Combined H5ND inactivated vaccine protects chickens against challenge by different clades of highly pathogenic avian influenza viruses subtype H5 and virulent Newcastle disease virus

Ali

Safwat²,

Kilany³

et al. 2019

Vet World

View full text Add to dashboard Cite

Aim: The aim of the current study was to evaluate the efficacy of a trivalent-inactivated oil-emulsion vaccine against challenge by different clades highly pathogenic avian influenza (HPAI) viruses including HPAI-H5N8 and the virulent genotype VII Newcastle disease virus (NDV) (vNDV). Materials and Methods: The vaccine studied herein is composed of reassortant AI viruses rgA/Chicken/Egypt/ ME1010/2016 (clade 2.2.1.1), H5N1 rgA/Chicken/Egypt/RG-173CAL/2017 (clade 2.2.1.2), and "NDV" (LaSota NDV/ CK/Egypt/11478AF/11); all used at a concentration of 108 EID50/bird and mixed with Montanide-ISA70 oil adjuvant. Two-week-old specific pathogen free (SPF) chickens were immunized subcutaneously with 0.5 ml of the vaccine, and hemagglutination inhibition (HI) antibody titers were monitored weekly. The intranasal challenge was conducted 4 weeks post-vaccination (PV) using 106 EID50/0.1 ml of the different virulent HPAI-H5N1 viruses representing clades 2.2.1, 2.2.1.1, 2.2.1.2, 2.3.4.4b-H5N8, and the vNDV. Results: The vaccine induced HI antibody titers of >6log2 against both H5N1 and NDV viruses at 2 weeks PV. Clinical protection against all HPAI H5N1 viruses and vNDV was 100%, except for HPAI H5N1 clade-2.2.1 and HPAI H5N8 clade- 2.3.4.4b viruses that showed 93.3% protection. Challenged SPF chickens showed significant decreases in the virus shedding titers up to <3log10 compared to challenge control chickens. No virus shedding was detected 6 "days post-challenge" in all vaccinated challenged groups. Conclusion: Our results indicate that the trivalent H5ND vaccine provides significant clinical protection against different clades of the HPAI viruses including the newly emerging H5N8 HPAI virus. Availability of such potent multivalent oil-emulsion vaccine offers an effective tool against HPAI control in endemic countries and promises simpler vaccination programs.

show abstract

Section: Discussionmentioning

confidence: 99%

Combined H5ND inactivated vaccine protects chickens against challenge by different clades of highly pathogenic avian influenza viruses subtype H5 and virulent Newcastle disease virus

Ali

Safwat²,

Kilany³

et al. 2019

Vet World

View full text Add to dashboard Cite

show abstract

“…Prior to the European 2016–2017 epidemic and alike the incursion patterns of the previously described outbreaks, analogous HPAIV H5N8 clade 2.3.4.4b reassortants were detected as the causative agent for mass mortality in waterfowl at the Uvs‐Nuur Lake located between Mongolia and the Republic of Tyva (Russia) in late May–early June 2016, followed by further reports in the Kurgan region three months later and almost simultaneous reports from Tatarstan and Lake Chany in October 2016 (Lee, Sharshov, et al., 2017; Li et al., 2017; Marchenko et al., 2017; Voronina et al., 2018). Due to the genetic characteristics of the novel clade 2.3.4.4b, the close relations to Central Asian isolates described and the lack of identification of other HPAIV H5Nx strains in wild birds from November 2014 to January 2016 in the Netherlands (Poen et al., 2016, 2018), the 2016–2017 epidemic most likely represents at least one new incursion into Europe.…”

Section: –2017: Clade 2344b—h5n8 H5n5mentioning

confidence: 99%

“…Phylogenetic analyses show 98% sequence identity of the segments PB2, PA, HA, NA, MP and NS to a preceding H5N8 reassortant previously detected on multiple continents during 2016/2017 (Figure S2, Table S2). This includes Asia/Eurasia, Europe and Africa (Abolnik et al., 2019; Fusaro et al., 2017; Kwon et al., 2018; Nagarajan et al., 2017; Voronina et al., 2018; Yehia et al., 2018). Slightly higher identity levels of 98.9%‐99.6% were detected for the PB2, PA, HA, MP and NS segments in combination with a Nigerian HPAIV H5N8 isolate detected in July 2019 (Adlhoch et al., 2020).…”

Section: : Clade 2344b H5n8—reassortant Ger‐01–20mentioning

confidence: 99%

The genetics of highly pathogenic avian influenza viruses of subtype H5 in Germany, 2006–2020

King

Harder

Conraths

et al. 2020

Transbound Emerg Dis

View full text Add to dashboard Cite

show abstract

“…Finally, complete genome sequencing of the virus variant A/ duck/Moscow/4182-C/2010(H5N3) and its escape mutants was performed by MiSeq Illumina platform as described (Voronina et al, 2018). Genomes were assembled using the CLC Genomics Workbench v. 9.5.2 and 12.0.…”

Section: Selection Of Escape Mutants Escape Mutants Have Beenmentioning

confidence: 99%

Variability of nonpathogenic influenza virus H5N3 under immune pressure

Тимофеева¹,

Руднева²,

Садыкова³

et al. 2020

View full text Add to dashboard Cite

Mutations arising in influenza viruses that have undergone immune pressure may promote a successful spread of mutants in nature. In order to evaluate the variability of nonpathogenic influenza virus A/duck/Moscow/4182-C/2010(H5N3) and to determine the common epitopes between it and highly pathogenic H5N1 avian influenza viruses (HPAIV), a set of escape mutants was selected due to action of MABs specific against A/chicken/Pennsylvania/8125/83(H5N2), A/Vietnam/1203/04(H5N1) and A/duck/ Novosibirsk/56/05(H5N1) viruses. The complete genomes of escape mutants were sequenced and amino acid point mutations were determined in HA, NA, PA, PB1, PB2, M1, M2, and NP proteins. Comprehensive analysis of the acquired mutations was performed using the Influenza Research Database (https://www. fludb.org) and revealed that all mutations were located inside short linear epitopes, in positions characterized by polymorphisms. Most of the mutations found were characterized as substitutions by predominant or alternative amino acids existing in nature. Antigenic changes depended only on substitutions at positions 126, 129, 131, 145 and 156 of HA (H3 numbering). The positions 126, 145 and 156 were common for HA/H5 of different phylogenetic lineages of H5N1 HPAIV (arisen from A/goose/Guangdong/1/96) and low pathogenic American and Eurasian viruses. Additionally, mutation S145P increased the temperature of HA heat inactivation, compared to wild-type, as was proved by reverse genetics. Moreover, nonpathogenic A/ duck/Moscow/4182-C/2010(H5N3) and H5N1 HPAI viruses have the same structure of short linear epitopes

show abstract

Genetic features of highly pathogenic avian influenza viruses A(H5N8), isolated from the European part of the Russian Federation

Cited by 10 publications

References 23 publications

Combined H5ND inactivated vaccine protects chickens against challenge by different clades of highly pathogenic avian influenza viruses subtype H5 and virulent Newcastle disease virus

Combined H5ND inactivated vaccine protects chickens against challenge by different clades of highly pathogenic avian influenza viruses subtype H5 and virulent Newcastle disease virus

The genetics of highly pathogenic avian influenza viruses of subtype H5 in Germany, 2006–2020

Variability of nonpathogenic influenza virus H5N3 under immune pressure

Contact Info

Product

Resources

About