Genetic exchange and reassignment in <i>Porphyromonas gingivalis</i>

Olsen, Ingar; Chen, Tsute; Tribble, Gena D.

doi:10.1080/20002297.2018.1457373

Cited by 16 publications

(13 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…P. gingivalis is a host adapted organism with a nonclonal population structure, and isolates from different individuals can vary extensively (27)(28)(29). Hence, we next examined the ability of different strains of P. gingivalis to enhance ZEB2 mRNA levels.…”

Section: Regulation Of Emt-inducing Transcription Factors By P Gingimentioning

confidence: 99%

Streptococcus gordonii programs epithelial cells to resist ZEB2 induction by Porphyromonas gingivalis

Ohshima

Wang

Fitzsimonds

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

The polymicrobial microbiome of the oral cavity is a direct precursor of periodontal diseases, and changes in microhabitat or shifts in microbial composition may also be linked to oral squamous cell carcinoma. Dysbiotic oral epithelial responses provoked by individual organisms, and which underlie these diseases, are widely studied. However, organisms may influence community partner species through manipulation of epithelial cell responses, an aspect of the host microbiome interaction that is poorly understood. We report here thatPorphyromonas gingivalis, a keystone periodontal pathogen, can up-regulate expression of ZEB2, a transcription factor which controls epithelial–mesenchymal transition and inflammatory responses. ZEB2 regulation byP. gingivaliswas mediated through pathways involving β-catenin and FOXO1. Among the community partners ofP. gingivalis,Streptococcus gordoniiwas capable of antagonizing ZEB2 expression. Mechanistically,S. gordoniisuppressed FOXO1 by activating the TAK1-NLK negative regulatory pathway, even in the presence ofP. gingivalis. Collectively, these results establishS. gordoniias homeostatic commensal, capable of mitigating the activity of a more pathogenic organism through modulation of host signaling.

show abstract

Section: Regulation Of Emt-inducing Transcription Factors By P Gingimentioning

confidence: 99%

Streptococcus gordonii programs epithelial cells to resist ZEB2 induction by Porphyromonas gingivalis

Ohshima

Wang

Fitzsimonds

et al. 2019

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

show abstract

“…In the study by Chen et al [23] who did comparative genomics of 19 P. gingivalis strains, a high prevalence of transposase proteins was found encoded in P. gingivalis (Table 2); actually as much as 149 copies in strain A7436. In another study transposases were found in all of the 35 genomes of P. gingivalis examined, varying in number from 8 (strains Ando, F0185, SDJ5) to 103 (A7436) [28]. The lower number of transposases detected in the original genomes that were not completely sequenced in this study was most likely due to the in-between-contig sequence gaps that may contain highly repeated sequences such as transposases and IS elements.…”

Section: Crisprs Provide Immunity Against Transposonsmentioning

confidence: 51%

“…In contrast, Cas proteins were found only in select strains. Of 35 strains of P. gingivalis examined strain 13_1 encoded as many as 19 Cas homologous proteins and five different CRISPR systems were observed [28]. On the other hand, JSVI_ SC001, which is an environmental strain, contained no detectable proteins even though three CRISPR-like DNA sequence structures were detected.…”

Section: Cas Proteins Do Not Necessarily Follow Crisprs In P Gingivalismentioning

confidence: 99%

Porphyromonas gingivalis and its CRISPR-Cas system

Chen

Olsen

2019

Journal of Oral Microbiology

Self Cite

View full text Add to dashboard Cite

The clustered regularly interspaced short palindromic repeats (CRISPRs) and their associated proteins (Cas) are immune systems in prokaryotes present in most Bacteria and Archaea. They provide adaptive immunity against foreign elements such as bacteriophages/viruses, plasmids and transposons. During immunization a small sequence of foreign DNA, a so-called spacer is integrated into the CRISPR locus in the host cell. Spacers are then transcribed into small RNA guides that direct cleavage of foreign DNA by Cas nucleases. Immunization through spacer acquisition is transferred vertically to the progeny. It is possible that this genetic immune system of bacteria participates in modulating the microbiome of 'chronic' periodontitis, in which Porphyromonas gingivalis has been identified as a keystone pathogen causing microbial dysbiosis. An in-depth review of our current knowledge on the CRISPR-Cas systems in P. gingivalis is given in this paper with the attempt to understand how this anaerobic bacterium may protect itself in the periodontal pocket where bacteriophages are abundant and even out-number bacteria. ARTICLE HISTORY

show abstract

“…The rag locus also contributes to host cell invasion by P. gingivalis 27 and soft tissue damage in a murine abscess model 28 . A definitive assignment of the role of the rag locus in virulence is complicated by the fact that clinical strains of P. gingivalis carry allelic forms of established virulence factors such as fimbria, that are known to be variably associated with the severity of periodontitis 29 . RagAB occurs in 4 well-defined allelic forms ( rag-1 , -2 , -3 and -4 ) 20 .…”

Section: Discussionmentioning

confidence: 99%

Dynamic oligopeptide acquisition by the RagAB transporter fromPorphyromonas gingivalis

Madej

Jbr

Nowakowska

et al. 2019

Preprint

View full text Add to dashboard Cite

38Porphyromonas gingivalis, an asaccharolytic Bacteroidetes, is a keystone pathogen in human 39 periodontitis that may also contribute to the development of other chronic inflammatory diseases, 40 such as rheumatoid arthritis, cardiovascular disease and Alzheimer's disease. P. gingivalis utilizes 41 protease-generated peptides derived from extracellular proteins for growth, but how those peptides 42 enter the cell is not clear. Here we identify RagAB as the outer membrane importer for peptides. X-43 ray crystal structures show that the transporter forms a dimeric RagA 2 B 2 complex with the RagB 44 substrate binding surface-anchored lipoprotein forming a closed lid on the TonB-dependent 45 transporter RagA. Cryo-electron microscopy structures reveal the opening of the RagB lid and thus 46 provide direct evidence for a "pedal bin" nutrient uptake mechanism. Together with mutagenesis, 47 peptide binding studies and RagAB peptidomics, our work identifies RagAB as a dynamic OM 48 oligopeptide acquisition machine with considerable substrate selectivity that is essential for the 49 efficient utilisation of proteinaceous nutrients by P. gingivalis. 50 51 Introduction 52 The Gram-negative Bacteroidetes are abundant members of the human microbiota, especially in 53 the gut. Outside the gut, Bacteroidetes often cause disease, with the best-known examples being 54 the oral Bacteroidetes Porphyromonas gingivalis and Tannerella forsythia that are part of the "red 55 complex" involved in periodontitis 1 , the most prevalent infection-driven chronic inflammation in the 56 Western world 2 . Accumulating evidence suggests a link between periodontitis and other chronic 57 inflammatory diseases, including rheumatoid arthritis, Alzheimer's disease, chronic obstructive 58 pulmonary disease and cardiovascular disease 3-7 . Given this link, and the fastidious growth 59 requirements of P. gingivalis, it is important to understand how this key pathogen thrives and 60 causes dysbiosis of the oral microbiota in the biofilm on the tooth surface below the gum line, 61 leading to inflammation and periodontal tissue destruction. 63Unlike many human gut Bacteroidetes that specialise in degrading complex host and dietary 64 glycans, P. gingivalis is asaccharolytic and exclusively utilises peptides for growth 8 . Those 65 peptides are generated by multiple proteases, including several secreted endopeptidases and 66 periplasmic di-and tri-peptidyl-peptidases 9,10 . The best-known P. gingivalis endopeptidases are the 67 gingipains, large and abundant surface-anchored cysteine proteases with cumulative trypsin-like 68 activity, which are essential for P. gingivalis virulence and growth on proteins as the sole source of 69 carbon 11 . Crucially, it is not clear how gingipain-generated peptides are taken up by P. gingivalis. A 70 role in this process has been proposed for the RagAB outer membrane (OM) protein complex 12 , 71 which consists of a TonB dependent transporter (TBDT) RagA (PG_0185) and a substrate binding72 surface lipoprotein RagB (PG_0186...

show abstract

Genetic exchange and reassignment in Porphyromonas gingivalis

Cited by 16 publications

References 56 publications

Streptococcus gordonii programs epithelial cells to resist ZEB2 induction by Porphyromonas gingivalis

Streptococcus gordonii programs epithelial cells to resist ZEB2 induction by Porphyromonas gingivalis

Porphyromonas gingivalis and its CRISPR-Cas system

Dynamic oligopeptide acquisition by the RagAB transporter fromPorphyromonas gingivalis

Contact Info

Product

Resources

About