Genetic Evolution of the Spanish Multidrug-Resistant
            <i>Salmonella enterica</i>
            4,5,12:i:- Monophasic Variant

Laorden, Lorena; Herrera-León, Silvia; Martinez, Ilargi; Sanchez, Ainhoa; Kromidas, Lefteris; Bikandi, Joseba; Rementerı́a, Aitor; Echeita, A.; Garaizar, Javier

doi:10.1128/jcm.00337-10

Cited by 40 publications

(40 citation statements)

References 18 publications

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“…Another study carried out with some Salmonella (4,5,12 :-) isolates from the United States and Spain concluded that this strain most likely represents multiple clones with distinct geographical distributions that emerged through independent deletion events (Soyer et al, 2009). This hypothesis was supported by another study and expanded the information given by Soyer et al, (Soyer et al, 2009) about Spanish monophasic strains, as they studied a larger number of strains and sequenced the fragments flanking the fljAB deletions (Laorden et al, 2010). …”

Section: Salmonella Sppsupporting

confidence: 70%

Pulsed-Field Gel Electrophoresis as a Molecular Tool for Characterizing Genomes of Certain Food-Borne Bacterial Isolates - A Review

Pal¹

2014

ILNS

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The evolutionary transition from phenotypic to molecular analysis of infectious disease in bacterial epidemiology led to the search for suitable approaches to ascertain genomic relatedness or heterogeneity between bacterial clinical isolates. Pulsed-field gel electrophoresis (PFGE) technique was developed for separating and analyzing long DNA fragments of several megabases in alternating electric field. Comparison of electrophoresis profiles of restriction enzyme-digested genomic DNA from bacterial isolates has proved to be a useful epidemiological tool for genetic discrimination of bacterial strains, detection of genetic relatedness, to locate the source of outbreak and to monitor the spread of the microorganisms in endemic zones. PFGE is considered as a gold standard method for typing of bacterial isolates because of the remarkable endurance of this technique as a typing method for the last 20 years in molecular epidemiology. In this current review the pros and cons of PFGE use in current molecular microbiological research are explored in the context of determination of genome organization of certain food-borne bacterial isolates causing infectious diseases in human beings.

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Section: Salmonella Sppsupporting

confidence: 70%

Pulsed-Field Gel Electrophoresis as a Molecular Tool for Characterizing Genomes of Certain Food-Borne Bacterial Isolates - A Review

Pal¹

2014

ILNS

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“…In the US clone sequence, 76 CDSs were absent including the fljBA operon and Fels-2 gene ). On the other hand, four different types of genomic deletions were characterized in S strains but it is not clear if they represent variants of a unique bacterial clone or if they arose independently (Laorden et al, 2010).…”

Section: Discussionmentioning

confidence: 99%

“…This suggests that S. enterica 4, [5],12:i:-is represented by multiple clones with distinct geographical distribution Laorden et al, 2010). Soyer et al (2009) suggested that deletion of the fljBA operon in the US clone could have resulted from an imprecise excision of a prophage.…”

Section: Introductionmentioning

confidence: 99%

“…A number of studies have demonstrated that S. enterica 4, [5],12:i:-is a variant of serovar Typhimurium due to the loss of the fljBA operon, which is responsible for flagellar phase variation (Echeita et al, 2001;Garaizar et al, 2002;Soyer et al, 2009;Laorden et al, 2010). In serovar Typhimurium, the two flagellar antigens are encoded by two structural genes, fliC (flagellar phase I) and fljB (flagellar phase II) .…”

Section: Introductionmentioning

confidence: 99%

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Salmonella enterica Typhimurium fljBA operon stability: implications regarding the origin of Salmonella enterica I 4,[5],12:i:-

et al. 2015

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ABSTRACT. Salmonella enterica subsp enterica serovar 4,5,12:i:-has been responsible for many recent Salmonella outbreaks worldwide. Several studies indicate that this serovar originated from S. enterica subsp enterica serovar Typhimurium, by the loss of the flagellar phase II gene (fljB) and adjacent sequences. However, at least two different clones of S. enterica 4,5,12:i:-exist that differs in the molecular events responsible for fljB deletion. The aim of this study was to test the stability of the fljBA operon responsible for the flagellar phase variation under different growth conditions in order to verify if its deletion is a frequent event that could explain the origin and dissemination of this serovar. In fact, coding sequences for transposons are present near this operon and in some strains, such as S. enterica Typhimurium LT2, the Fels-2 prophage gene is inserted near this operon. The presence of mobile DNA could confer instability to this region. In order to examine this, the cat (chloramphenicol acetyltransferase) gene was inserted adjacent to the fljBA operon so that deletions involving this genomic region could be identified. After growing S. enterica chloramphenicol-resistant strains under different conditions, more ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (4): 19057-19065 (2015) than 10 4 colonies were tested for the loss of chloramphenicol resistance. However, none of the colonies were sensitive to chloramphenicol. These data suggest that the origin of S. enterica serovar 4,5,12:i:-from Typhimurium by fljBA deletion is not a frequent event. The origin and dissemination of 4,5,12:i:-raise several questions about the role of flagellar phase variation in virulence.

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“…In Salmonella strains, namely S. Typhimurium and the monophasic variant S. 4 [5],12:i:-, IS26 elements have been linked to the presence of resistance regions conferring an R-type ASSuT resistance pattern [7,8].…”

Section: Introductionmentioning

confidence: 99%

First description of food-borne Salmonella enterica resistance regions R1 and R3 associated with IS26 elements

Gomes-Neves

Manageiro

Ferreira

et al. 2015

Research in Microbiology

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In this study, we assessed the presence of IS26 in food-borne ASSuT-type Salmonella enterica isolates. A new genetic region (R3) was described, that included a C14 caspase gene between IS26 elements. R3 was present in two Salmonella Rissen isolates from a swine carcass and a meat handler, collected at the same abattoir. Furthermore, a new rearrangement of resistance region R1, harboring the bla TEM-1 gene flanked by IS26 elements, was identified in Salmonella Typhimurium and Salmonella 4, [5],12:i:-, from different samples. This study highlights the zoonotic potential of Salmonella spp. isolates and the possible role of IS26 in the mobilization of resistance genes.

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Genetic Evolution of the Spanish Multidrug-Resistant Salmonella enterica 4,5,12:i:- Monophasic Variant

Cited by 40 publications

References 18 publications

Pulsed-Field Gel Electrophoresis as a Molecular Tool for Characterizing Genomes of Certain Food-Borne Bacterial Isolates - A Review

Pulsed-Field Gel Electrophoresis as a Molecular Tool for Characterizing Genomes of Certain Food-Borne Bacterial Isolates - A Review

Salmonella enterica Typhimurium fljBA operon stability: implications regarding the origin of Salmonella enterica I 4,[5],12:i:-

First description of food-borne Salmonella enterica resistance regions R1 and R3 associated with IS26 elements

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