Genetic evaluation of interindividual relatedness for broodstock management of the rare species barfin flounder Verasper moseri using microsatellite DNA markers

Abstract

The barfin flounder has been recently declared to be rare, as the number of wild individuals in Japan has greatly reduced since 1975. The Japanese National Center for Stock Enhancement started the stocking program for this species on 1987. In the present study, microsatellite DNA loci were applied in order to determine the pedigree of the hatchery-produced juveniles for stock enhancement. Additionally, using six polymorphic microsatellite markers, the accuracy to assign dyads to categories of kinship was estimated using the relatedness estimator (R XY ), similarity indexes (individual genetic identity [GI] and proportion of shared alleles [P S ]), identification of kin groups using unweighted pair group method with arithmetic mean (UPGMA) clustering based on interindividual genetic distances (1-R XY , 1-P S , and 1-GI), a likelihood ratio approach, and partition methods to separate individuals into cohorts. The results were compared with the pedigree relationship previously obtained from parentage analysis. Estimation of the kinship relationship between individuals could be better inferred by means of UPGMA dendrogram clustering based on 1-GI or 1-P S as interindividual genetic distances, or using computer software to perform partitioning of set of individuals.

Genetic analysis of gynogenetic and common populations of Verasper moseri using SSR markers

“…Currently, molecular markers [16][17][18] , especially microsatellite markers, have been widely used in many fish species for analyzing genetic diversity [19][20][21] , mapping of genes [22] , and screening of sex-specific markers [23] because of their many ideal traits, such as high polymorphism, ease of assay, and exhibition of codominant Mendelian inheritance. Microsatellite markers have been isolated in barfin flounder (Verasper moseri) [24] , and the genetic population diversity and interindividual relatedness for broodstock management were evaluated [25,26] . Besides that, data about the application of molecular markers in barfin flounder is limited.…”

Germplasm differences among three species of genus Erythroculter based on morphological characters and gene sequences of mitochondrial CO I and Cyt b

“…Genetic diversity is of great importance to the viability of populations (Qi, et al, 2013). To protect and sustainably exploit the natural resource, population genetic research (genetic structure, population divergence and genetic diversity) is needed to provide a guidance for resource conservation and management (Ortega-Villaizán Romo, Suzuki, Nakajima, & Taniguchi, 2006). A better understanding of the genetic resources will not only help to protect the fish resource but also facilitate the development of sustainable fishing strategies (Wang, et al, 2017).…”

Parentage assignment in hatchery population of brown sole Pleuronectes herzensteini by microsatellite DNA markers

“…Thus, these markers have been used extensively in various aspects of aquatic organisms including gene mapping, allocation of parentage, kinships, and stock structure in the field of fisheries science 3 . Recently, microsatellite DNA markers have also been actively developed and widely used for genetic studies of natural populations, 4–6 parentage assignment, 7–12 and the management of resources of various kinds of commercially important aquatic animals 13–18 …”

“…3 Recently, microsatellite DNA markers have also been actively developed and widely used for genetic studies of natural populations, 4-6 parentage assignment, [7][8][9][10][11][12] and the management of resources of various kinds of commercially important aquatic animals. [13][14][15][16][17][18] Brown sole Pleuronectes herzensteini is an important species as an inshore fishery resource in Japan. Recently, stock enhancement by release has been expanded, because optimal conditions for rearing and feeding brown sole larvae have been clarified, and artificial production of seedlings has been technically established by the Hokkaido Mariculture Fisheries Experiment Station (formerly Hokkaido Institute of Mariculture), Japan.…”