Genetic Enhancement of Tomato Crop for Abiotic Stress Tolerance

Sadashiva, A. T.; Christopher, M George; Krithika, Thilakadavu Krishnamurthy

doi:10.1007/978-81-322-0974-4_11

Cited by 5 publications

(4 citation statements)

References 92 publications

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“…However, their wild types such as L. pimpinellifolium , L. pennellii , L. chilense and L. esculentum var. cerasiforme are drought‐tolerant species (Sadashiva et al ., ). Researches are in progress to clarify molecular pathways on drought response of plants (Sadashiva et al ., ), and miRNAs can be alternatives to identify drought metabolism, as they play crucial role on abiotic stress response regulating the stress‐related mRNAs (Kumar, ).…”

Section: Introductionmentioning

confidence: 97%

“…cerasiforme are drought‐tolerant species (Sadashiva et al ., ). Researches are in progress to clarify molecular pathways on drought response of plants (Sadashiva et al ., ), and miRNAs can be alternatives to identify drought metabolism, as they play crucial role on abiotic stress response regulating the stress‐related mRNAs (Kumar, ). Determination and functional characterization of stress‐related miRNAs and target identification are important in breeding programmes and can contribute to developing new strategies for improving stress tolerance (Barrera‐Figueroa et al ., ).…”

Section: Introductionmentioning

confidence: 97%

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Small RNA and degradome deep sequencing reveals drought‐and tissue‐specific micrornas and their important roles in drought‐sensitive and drought‐tolerant tomato genotypes

Candar-Cakir

Arican

Zhang

2016

Plant Biotechnology Journal

159

102

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SummaryDrought stress has adverse impacts on plant production and productivity. MicroRNAs (miRNAs) are one class of noncoding RNAs regulating gene expression post‐transcriptionally. In this study, we employed small RNA and degradome sequencing to systematically investigate the tissue‐specific miRNAs responsible to drought stress, which are understudied in tomato. For this purpose, root and upground tissues of two different drought‐responsive tomato genotypes (Lycopersicon esculentum as sensitive and L. esculentum var. cerasiforme as tolerant) were subjected to stress with 5% polyethylene glycol for 7 days. A total of 699 conserved miRNAs belonging to 578 families were determined and 688 miRNAs were significantly differentially expressed between different treatments, tissues and genotypes. Using degradome sequencing, 44 target genes were identified associated with 36 miRNA families. Drought‐related miRNAs and their targets were enriched functionally by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. Totally, 53 miRNAs targeted 23 key drought stress‐ and tissue development‐related genes, including DRP (dehydration‐responsive protein), GTs (glycosyltransferases), ERF (ethylene responsive factor), PSII (photosystem II) protein, HD‐ZIP (homeodomain‐leucine zipper), MYB and NAC‐domain transcription factors. miR160, miR165, miR166, miR171, miR398, miR408, miR827, miR9472, miR9476 and miR9552 were the key miRNAs functioning in regulation of these genes and involving in tomato response to drought stress. Additionally, plant hormone signal transduction pathway genes were differentially regulated by miR169, miR172, miR393, miR5641, miR5658 and miR7997 in both tissues of both sensitive and tolerant genotypes. These results provide new insight into the regulatory role of miRNAs in drought response with plant hormone signal transduction and drought‐tolerant tomato breeding.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 97%

Small RNA and degradome deep sequencing reveals drought‐and tissue‐specific micrornas and their important roles in drought‐sensitive and drought‐tolerant tomato genotypes

Candar-Cakir

Arican

Zhang

2016

Plant Biotechnology Journal

159

102

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“…Solanum lycopersicum is an important vegetable crop, and its growth and productivity are strongly affected by abiotic stresses, such as salt or drought, worldwide (Duan et al , Sadashiva et al ). Therefore, identifying novel genes involved in this process and understanding their functions can improve plant stress tolerance.…”

Section: Discussionmentioning

confidence: 99%

A homologue of vitamin K epoxide reductase in Solanum lycopersicum is involved in resistance to osmotic stress

Yang

et al. 2015

Physiologia Plantarum

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Vitamin K epoxide reductase (VKOR) exists widely in the chloroplasts of higher plants and plays important roles in redox regulation. However, investigations of plant VKOR function have primarily focused on VKOR from Arabidopsis, and knowledge concerning this function is therefore quite limited. In this study, Solanum lycopersicum was used to study the function of VKOR. Transcription level analysis revealed that SlVKOR (S. lycopersicum VKOR) expression was upregulated by salt or drought stress. To further investigate the function of SlVKOR in plants, we generated sense and antisense transgenic S. lycopersicum homozygotes at T2 generation plants. Compared with wild-type (WT) plants, the leaf disks of the SlVKOR overexpression plants retained a much higher percentage of chlorophyll after salt or drought treatment, whereas the antisense transgenic plants displayed an opposite response. The overexpressed plants displayed lower levels of H2O2 and superoxide anion radical (O2(·-)) than WT plants, whereas antisense plants accumulated considerably more H2O2 and O2(·-). The activities of reactive oxygen scavenger enzymes, including superoxide dismutase, peroxidase, ascorbate peroxidase and catalase, were consistent with the accumulation of reactive oxygen species. Based on these results, we suggest that SlVKOR is involved in resistance to salt or drought stress.

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“…cerasiforme Peru), RF4A (S. pennellii IIHR India), etc. which can be used in the breeding programs to develop drought resistant varieties of tomato [86]. Tests for drought stress tolerance shows that under the drought stress conditions S. pennellii has good ability to increase the water use efficiency when compared to cultivated S. lycopersicum.…”

Section: A Way Forwardmentioning

confidence: 99%

Growth and Physiological Responses of Vegetable Crops to Water Deficit Stress

Vashi

Patel

Bardhan

2020

JEAI

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Future climate change scenarios have become a major threat and limitation for global food production. The rise in greenhouse gases and temperature throughout the globe has caused perturbance in the natural seasonal cycles. This causes a threat to nutritional and calorific food security for the ever-increasing population in the South Asian Countries. Reduction in the amounts of rainfall in the tropics and subtropics has increased the occurrence of drought leading to more frequent water stress affecting the production of crops, including vegetables, which are predominantly grown in the tropical and sub-tropical regions. India is the second-largest producer of vegetables in the world and heavy consumer of vegetables. The mini-review focused on drought stress effects on the vegetable production and photosynthesis, transpiration, water uptake and other metabolic and developmental processes. Grafting, breeding, cultural and biotechnological strategies are used to combat drought stress and will be used in the future to ease the harmful effects of drought stress.

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Genetic Enhancement of Tomato Crop for Abiotic Stress Tolerance

Cited by 5 publications

References 92 publications

Small RNA and degradome deep sequencing reveals drought‐and tissue‐specific micrornas and their important roles in drought‐sensitive and drought‐tolerant tomato genotypes

Small RNA and degradome deep sequencing reveals drought‐and tissue‐specific micrornas and their important roles in drought‐sensitive and drought‐tolerant tomato genotypes

A homologue of vitamin K epoxide reductase in Solanum lycopersicum is involved in resistance to osmotic stress

Growth and Physiological Responses of Vegetable Crops to Water Deficit Stress

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