Genetic engineering ruminal stable high methionine protein in the foliage of alfalfa

Bagga, Suman; Armendaris, Angela; Klypina, Nina; Ray, I. M.; Ghoshroy, Soumitra; Endress, Marcia; Sutton, Dennis W.; Kemp, John D.; Sengupta‐Gopalan, Champa

doi:10.1016/j.plantsci.2003.09.014

Cited by 21 publications

(25 citation statements)

References 36 publications

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“…Notably, in these CGS-overexpressing alfalfa plants, Met incorporated into the watersoluble protein fraction was also significantly increased, indicating that in the vegetative tissues of alfalfa plants, soluble Met content represents a limiting factor for its incorporation into endogenous sulfur-rich proteins. These transgenic plants may therefore contain an adequate Met content in their vegetative tissues to feed animals, without any need for the addition of synthetic Met , which is usually supplemented to complete the sulfur content of animal feeds (Bagga et al, 2003(Bagga et al, , 2004.…”

Section: Metabolic Engineering Of Met Biosynthesis Towards Improving mentioning

confidence: 99%

“…Nevertheless, the levels of these ER-localised proteins were not increased above 1.3% of the total soluble protein in the vegetative tissues. A higher accumulation level was obtained when b-, g-and/or d-zein, all of which accumulate naturally in seed ERderived protein bodies, were expressed in the vegetative tissue of forage and non-forage plants, such as alfalfa, lotus, tobacco and white clover (Bagga et al, 1997(Bagga et al, , 2004Bellucci et al, 1997Bellucci et al, , 2002Sharma et al, 1998). Notably, tobacco and alfalfa plants overexpressing the band d-zein produced novel ER-derived protein bodies in leaves, which apparently protect them from degradation (Bagga et al, 1995(Bagga et al, , 2004.…”

Section: Improving Met Content By Expressing Sulfur-rich Proteinsmentioning

confidence: 99%

“…A higher accumulation level was obtained when b-, g-and/or d-zein, all of which accumulate naturally in seed ERderived protein bodies, were expressed in the vegetative tissue of forage and non-forage plants, such as alfalfa, lotus, tobacco and white clover (Bagga et al, 1997(Bagga et al, , 2004Bellucci et al, 1997Bellucci et al, , 2002Sharma et al, 1998). Notably, tobacco and alfalfa plants overexpressing the band d-zein produced novel ER-derived protein bodies in leaves, which apparently protect them from degradation (Bagga et al, 1995(Bagga et al, , 2004. Co-expression of these two proteins also significantly increased further the level and stability of d-zein (Bagga et al, 1995(Bagga et al, , 1997(Bagga et al, , 2004, implying that interactions between different zeins may be important for their accumulation (Kim et al, 1992).…”

Section: Improving Met Content By Expressing Sulfur-rich Proteinsmentioning

confidence: 99%

“…Notably, tobacco and alfalfa plants overexpressing the band d-zein produced novel ER-derived protein bodies in leaves, which apparently protect them from degradation (Bagga et al, 1995(Bagga et al, , 2004. Co-expression of these two proteins also significantly increased further the level and stability of d-zein (Bagga et al, 1995(Bagga et al, , 1997(Bagga et al, , 2004, implying that interactions between different zeins may be important for their accumulation (Kim et al, 1992). To further test whether the level of free Met limits the production of sulfur-rich proteins in vegetative tissues, transgenic alfalfa plants constitutively expressing the 15-kDa zein were crossed with those described above that constitutively express Arabidopsis CGS (Bagga et al, 2003(Bagga et al, , 2004Golan et al, 2005).…”

Section: Improving Met Content By Expressing Sulfur-rich Proteinsmentioning

confidence: 99%

“…Co-expression of these two proteins also significantly increased further the level and stability of d-zein (Bagga et al, 1995(Bagga et al, , 1997(Bagga et al, , 2004, implying that interactions between different zeins may be important for their accumulation (Kim et al, 1992). To further test whether the level of free Met limits the production of sulfur-rich proteins in vegetative tissues, transgenic alfalfa plants constitutively expressing the 15-kDa zein were crossed with those described above that constitutively express Arabidopsis CGS (Bagga et al, 2003(Bagga et al, , 2004Golan et al, 2005). Compared to plants expressing only the 15-kDa zein, those co-expressing both transgenes showed significantly enhanced levels of the maize 15-kDa zein, concurrent with a reduction in the level of soluble Met, implying that in the crossed plants more soluble Met was incorporated into the 15-kDa zein (Bagga et al, 2003(Bagga et al, , 2004Golan et al, 2005).…”

Section: Improving Met Content By Expressing Sulfur-rich Proteinsmentioning

confidence: 99%

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Improving the levels of essential amino acids and sulfur metabolites in plants

Galili

Amir

Hoefgen

et al. 2005

Biological Chemistry

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Plants represent the major source of food for humans, either directly or indirectly through their use as livestock feeds. Plant foods are not nutritionally balanced because they contain low proportions of a number of essential metabolites, such as vitamins and amino acids, which humans and a significant proportion of their livestock cannot produce on their own. Among the essential amino acids needed in human diets, Lys, Met, Thr and Trp are considered as the most important because they are present in only low levels in plant foods. In the present review, we discuss approaches to improve the levels of the essential amino acids Lys and Met, as well as of sulfur metabolites, in plants using metabolic engineering approaches. We also focus on specific examples for which a deeper understanding of the regulation of metabolic networks in plants is needed for tailor-made improvements of amino acid metabolism with minimal interference in plant growth and productivity.

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Section: Metabolic Engineering Of Met Biosynthesis Towards Improving mentioning

confidence: 99%