Genetic engineering of the β-oxidation pathway in the yeast Yarrowia lipolytica to increase the production of aroma compounds

Abstract

The yeast Yarrowia lipolytica possesses five acyl-CoA oxidases (Aox1p to 5), the enzyme catalysing the first reaction of ␤-oxidation. The understanding of the specific role of each acyl-CoA oxidase is important to construct a yeast strain growing at a good rate and able to produce without degrading the aroma compound ␥-decalactone. In this study we observed that Aox4p exhibits a slight activity on a broad spectrum of substrates and that it is involved in lactone degradation. We constructed a strain lacking this activity. Its growth was only slightly altered and it produced 10 times more lactone than the wild type in 48 h.

Exploring medium-chain-length polyhydroxyalkanoates production in the engineered yeast Yarrowia lipolytica

“…In the last decades, the unconventional yeast Y. lipolytica (originally classified as Candida lipolytica) has attracted much interests, notably due to its excellent lipid/ fatty acid catabolism and anabolism capacity depending on the cultivation mode [4,9]. Therefore, it is an interesting microorganism for lipid synthesis or utilization to produce high-value bioproducts [7].…”

Efficient biosynthesis of γ-decalactone in ionic liquids by immobilized whole cells of Yarrowia lipolytica G3-3.21 on attapulgite

“…The biotransformation of GDL was initiated by adding the beads (0.53 g/mL) to the castor oil solution (50 g/L) at 26°C and pH 8.5. After 48 h of reaction under stirring at 150 rpm, the product of GDL was extracted with 30 lL of diethyl ether and analyzed as described by Groguenin et al [17] by a HP6890 gas chromatography (Agilent Technologies, USA) equipped with a flame ionization detector and a HP-INNOWax capillary column (30 m 9 0.32 mm i.d., film thickness 0.25 lm, Agilent). N 2 was used as carrier gas at a linear flow rate of 4.5 mL/ min.…”

Production of Dicarboxylic Acids and Flagrances by Yarrowia lipolytica

“…The case of Y. lipolytica is somewhat particular due to the presence of six isoforms of acyl-CoA oxidases with different substrate selectivity (Wang et al 1999a, b). A strategy was thus carried out by sequential disruptions to study the role of each acyl-CoA oxidase in the catabolism of ricinoleic acid and the production of γ-decalactone (Pagot et al 1998;Waché et al 2000Waché et al , 2001Waché et al , 2002Groguenin et al 2004). By deleting all the genes coding for acyl-CoA oxidases active on short-chain acyl-CoA, it was possible to increase notably the production of γ-decalactone and to avoid further degradation (Fig.…”

“…These lactones result from exit of metabolites at different steps of the β-oxidation cycle (Waché et al 2001(Waché et al , 2003Groguenin et al 2004;Escamilla García et al 2007a, b, 2009 (Fig. 7) with only the 3-keto-lactone lacking.…”