Genetic engineering of polyamine metabolism changes Medicago truncatula responses to water deficit

Duque, Ana Sofia; López‐Gómez, Miguel; Kráčmarová, J.; Gomes, Carolina; Araújo, Susana de Sousa; Lluch, Carmen; Fevereiro, Pedro

doi:10.1007/s11240-016-1107-1

Cited by 20 publications

(8 citation statements)

References 38 publications

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“…These results are in accordance with the identified branch pathway, which suggests that MtNGA1 plays an important role in the branch regulation pathway ( Figure 10). Plants are subject to many environmental stresses during their growth, and some transcription factors, enzymes, and metabolites [39] have been shown to be involved in the plant's response to adversity. To further study the function of the MtNGA1 gene, we tested the resistance of MtNGA1 transgenic Arabidopsis to stress.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, we examined the expression levels of the genes AtPLC1, AtPLC3, AtPLC4, AtPLC5, AtNCED3 and AtDI21 in transgenic MtNGA1 plants under normal conditions. The AtPLC genes are induced by various environmental stimuli, including cold, Plants are subject to many environmental stresses during their growth, and some transcription factors, enzymes, and metabolites [39] have been shown to be involved in the plant's response to adversity. To further study the function of the MtNGA1 gene, we tested the resistance of MtNGA1 transgenic Arabidopsis to stress.…”

Section: Discussionmentioning

confidence: 99%

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Expression of a NGATHA1 Gene from Medicago truncatula Delays Flowering Time and Enhances Stress Tolerance

Guo

Wang

et al. 2020

IJMS

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Shoot branching is one of the most variable determinants of crop yield, and the signaling pathways of plant branches have become a hot research topic. As an important transcription factor in the B3 family, NGATHA1 (NGA1), plays an important role in regulating plant lateral organ development and hormone synthesis and transport, but few studies of the role of this gene in the regulation of plant growth and stress tolerance have been reported. In this study, the NGA1 gene was isolated from Medicago truncatula (Mt) and its function was characterized. The cis-acting elements upstream of the 5′ end of MtNGA1 and the expression pattern of MtNGA1 were analyzed, and the results indicated that the gene may act as a regulator of stress resistance. A plant expression vector was constructed and transgenic Arabidopsis plants were obtained. Transgenic Arabidopsis showed delayed flowering time and reduced branching phenotypes. Genes involved in the regulation of branching and flowering were differentially expressed in transgenic plants compared with wild-type plants. Furthermore, transgenic plants demonstrated strong tolerances to salt- and mannitol-induced stresses, which may be due to the upregulated expression of NCED3 (NINE-CIS-EPOXYCAROTENOID DIOXYGENASE 3) by the MtNGA1 gene. These results provide useful information for the exploration and genetic modification use of MtNGA1 in the future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Expression of a NGATHA1 Gene from Medicago truncatula Delays Flowering Time and Enhances Stress Tolerance

Guo

Wang

et al. 2020

IJMS

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“…The authors reported increased putrescine content accumulation in the transgenic plants under drought stress that ultimately improved water balance of the cells by enhancing drought tolerance. In another study, Duque et al (2016) transferred the oat Adc gene of arginine decarboxylase (ADC), a key enzyme responsible for polyamine (PA) biosynthetic pathway to Medicago truncatula and reported increased drought tolerance in transgenic plants. In a recent study, Espasandin et al (2018) developed Lotus tenuis transgenic plants expressing ADC (pRD29A: oat arginine) decarboxylase gene.…”

Section: Ammonium Compound Group: Polyaminesmentioning

confidence: 99%

Osmoprotection in plants under abiotic stresses: new insights into a classical phenomenon

Zulfiqar

Akram

Ashraf

2019

Planta

190

112

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“…It has a small and almost completely annotated genome (500–550 Mbp) which is publicly available ( Goodstein et al, 2012 ), and it is more drought tolerant than other legume crops such as pea, bean, and soybean ( Motan et al, 1994 ; Gonzalez et al, 1998 ; Costa França et al, 2000 ; Galvez et al, 2005 ). In spite of this, previous studies on water stress resistance in M. truncatula mostly concerned gene transfer ( Alcântara et al, 2015 ; Araújo et al, 2015 ; Duque et al, 2016 ). The assessment of physiological responses ( Nunes et al, 2008 ) and their genetic mechanisms ( Badri et al, 2011 ) is more limited.…”

Section: Introductionmentioning

confidence: 99%

PEG Induces High Expression of the Cell Cycle Checkpoint Gene WEE1 in Embryogenic Callus of Medicago truncatula: Potential Link between Cell Cycle Checkpoint Regulation and Osmotic Stress

et al. 2017

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Polyethylene glycol (PEG) can be used to mimic osmotic stress in plant tissue cultures to study mechanisms of tolerance. The aim of this experiment was to investigate the effects of PEG (M.W. 6000) on embryogenic callus of Medicago truncatula. Leaf explants were cultured on MS medium with 2 mg L-1 NAA and 0.5 mg L-1 BAP for 5 months. Then, calli were transferred to the same medium further supplemented with 10% (w/v) 6000 PEG for 6 months in order to study physiological and putative molecular markers of water stress. There were no significant differences in growth rate of callus or mitotic index ± PEG although embryogenic potential of PEG treated callus was morphologically enhanced. Cells were rounder on PEG medium and cell size, nuclear size and endoreduplication increased in response to the PEG treatment. Significant increases in soluble sugar and proline accumulation occurred under PEG treatment compared with the control. Significantly, high MtWEE1 and MtCCS52 expression resulted from 6 months of PEG treatment with no significant differences in MtSERK1 or MtP5CS expression but down regulation of MtSOS expression. The results are consistent in showing elevated expression of a cell cycle checkpoint gene, WEE1. It is likely that the cell cycle checkpoint surveillance machinery, that would include WEE1 expression, is ameliorating the effects of the stress imposed by PEG.

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Genetic engineering of polyamine metabolism changes Medicago truncatula responses to water deficit

Cited by 20 publications

References 38 publications

Expression of a NGATHA1 Gene from Medicago truncatula Delays Flowering Time and Enhances Stress Tolerance

Expression of a NGATHA1 Gene from Medicago truncatula Delays Flowering Time and Enhances Stress Tolerance

Osmoprotection in plants under abiotic stresses: new insights into a classical phenomenon

PEG Induces High Expression of the Cell Cycle Checkpoint Gene WEE1 in Embryogenic Callus of Medicago truncatula: Potential Link between Cell Cycle Checkpoint Regulation and Osmotic Stress

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