Genetic engineering of Plum pox virus resistance: ‘HoneySweet’ plum—from concept to product

Scorza, R.; Callahan, Ann M.; Dardick, Chris; Ravelonandro, Michel; Polák, J.; Malinowski, Tadeusz; Zagrai, Ioan; Cambra, M.; Kamenova, I.

doi:10.1007/s11240-013-0339-6

Cited by 112 publications

(86 citation statements)

References 96 publications

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“…They are based on either directly targeting the PPV RNA genome through an RNA silencing approach (126) or inactivating host genes that are indispensable for the virus (147). However, such strategies rely on genetically modified plants whose production is, at present, neither fully accepted by the public nor fully effective because only a few accessions of European and Japanese plums are reproducibly transformed (126,139). In the near future, this might severely impede the development and deployment of biotechnology-based PPV-resistant prunus cultivars.…”

Section: Breeding Tools and The Selection Of Plum Pox Virus-resistantmentioning

confidence: 99%

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Rimbaud

Dallot

Gottwald

et al. 2015

Annu. Rev. Phytopathol.

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Many plant epidemics that cause major economic losses cannot be controlled with pesticides. Among them, sharka epidemics severely affect prunus trees worldwide. Its causal agent, Plum pox virus (PPV; genus Potyvirus), has been classified as a quarantine pathogen in numerous countries. As a result, various management strategies have been implemented in different regions of the world, depending on the epidemiological context and on the objective (i.e., eradication, suppression, containment, or resilience). These strategies have exploited virus-free planting material, varietal improvement, surveillance and removal of trees in orchards, and statistical models. Variations on these management options lead to contrasted outcomes, from successful eradication to widespread presence of PPV in orchards. Here, we present management strategies in the light of sharka epidemiology to gain insights from this worldwide experience. Although focused on sharka, this review highlights more general levers and promising approaches to optimize disease control in perennial plants.

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Section: Breeding Tools and The Selection Of Plum Pox Virus-resistantmentioning

confidence: 99%

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Rimbaud

Dallot

Gottwald

et al. 2015

Annu. Rev. Phytopathol.

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show abstract

“…Initially discovered in Bulgaria in the early 1900s, PPV is currently found in almost all European countries, the Middle East, Caucasus, Central Asia, North Africa, China, Canada, and the United States of America [1]. Known as sharka, this disease causes chlorotic spots, irregular lines or rings on leaves, as well as the deformation and malformation of fruits [2].…”

Section: Introductionmentioning

confidence: 99%

“…Current losses in Europe, where the virus is most prevalent, amount to $180 million each year [3]. Since the spread of the PPV virus in nurseries or orchards can occur in an unpredictable manner, it is expected that economic losses in various regions will continue to grow [1]. Taking into account the inability to cure infected plants through chemical treatment, the best approach to control sharka disease is to develop PPV resistant stone fruit trees.…”

Section: Introductionmentioning

confidence: 99%

“…In other words, the results of the unpredictable activation of RNA silencing against the PPV CP sequence that occurred was due to a duplication and rearrangement of CP gene copies during the transgene insertion [1]. By this time, it became obvious that the transgenic strategies based on the expression of sequences derived from the viral genome mainly resulted in unstable and unpredictable PPV resistance [1,4]. From the mid-2000s, the RNA silencing pathway has become a main tool for engineering virus resistant Prunus species.…”

Section: Introductionmentioning

confidence: 99%

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Generation of Transgenic Rootstock Plum ((Prunus pumila L. × P. salicina Lindl.) × (P. cerasifera Ehrh.)) Using Hairpin-RNA Construct for Resistance to the Plum pox virus

et al. 2017

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Abstract:The use of Prunus rootstocks that are resistant to plum pox virus (PPV) is an important agronomic strategy to combat the spread of the Sharka disease in nurseries and orchards. Despite remarkable progress in developing stone fruit rootstocks to adapt to various stresses, breeding that ensures durable virus resistance has not yet been achieved. For this reason, the engineering of PPV resistant plants through genetic transformation is a very promising approach to control sharka disease. The aim of the present study is to produce transgenic plants of the clonal rootstock 'Elita', which is resistant to PPV using ribonucleic acid interference (RNAi) technology. The genetic construct containing the self-complementary fragments of the plum pox virus coat protein (PPV-CP) gene sequence were used to induce the mechanism of post-transcriptional gene silencing to ensure virus resistance. Transgenic plants have been produced after agrobacterium-mediated transformation of in vitro explanted leaves. The results of polymerase chain reaction (PCR) and Southern blotting analyses confirmed the stable genomic integration of the PPV-CP sense and antisense intron-hairpin-RNA sequence. The functionality of the introduced expression cassette was confirmed by the activity of including the uidA gene into the transferring T-DNA. To our knowledge, this is the first interspecific plum rootstock produced by genetic engineering to achieve PPV resistance.

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“…In Romania, large surfaces are cultivated with different varieties of plum (Tuleu gras, Grase româneşti, Vinete româneşti, Stanley, Anna Späth, d' Agen, Vinete de Italia, Reine Claude d' Althan) and the top Romanian products after fruit industrialization is "ţuica de prune", a traditional Romanian brandy and "magiun", a DOC European recognized marmalade made from plums, with no added sugar. Due to their nutritive value and high amounts of bioactive compounds, plums are recommended as dietary fruits that should not be missing from our diet, also because of their low calories and many other health promoting compounds such as flavonoids, anthocyanins, carotenoids, minerals (Voća et al, 2009, Walkowiak-Tomczak, 2008 Scorza et al, 2013). During the ripening process, biochemical modifications lead to development of characteristic attributes of taste, colour, flavor and firmness.…”

Section: Introductionmentioning

confidence: 99%

Comparative Studies on Chemical Composition of Two Conventional Bred and One Genetically Engineered Plum-Fruits

Bobiş¹,

Zagrai²,

Bonta³

et al. 2017

BUASVMCN-ASB

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The European plum (Prunus domestica L.) is one of the most important cultivated species among fruit trees in the world, and the dominant in Romania. Regarding the development of new plum cultivars derived from Prunus domestica, the following characteristics are taken into consideration most of the time: high yield performance, good quality fruits and last but not least, the resistance to PPV. "HoneySweet" is a transgenic clone of Prunus domestica transformed with Plum pox virus coat protein gene making it highly resistant to the virus. This study presents preliminary results on the chemical composition of HoneySweet transgenic plum and two conventional ones: Reine Claude d' Althan and Stanley. The most important nutritional factors were determined. HoneySweet had balanced individual carbohydrates content but lower soluble solids in comparison with the two conventional cultivars. Total lipids content in HoneySweet fruits was similar to Stanley, higher than in Reine Claude d' Althan. HoneySweet is distinguished by the highest total protein content. Similar amounts of magnesium and calcium were recorded in the three varieties. Overall, our preliminary results revealed that the quality and nutritional value of "Honey Sweet" genetic engineered plum is in the range of other valuable plum cultivars.

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Genetic engineering of Plum pox virus resistance: ‘HoneySweet’ plum—from concept to product

Cited by 112 publications

References 96 publications

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Sharka Epidemiology and Worldwide Management Strategies: Learning Lessons to Optimize Disease Control in Perennial Plants

Generation of Transgenic Rootstock Plum ((Prunus pumila L. × P. salicina Lindl.) × (P. cerasifera Ehrh.)) Using Hairpin-RNA Construct for Resistance to the Plum pox virus

Comparative Studies on Chemical Composition of Two Conventional Bred and One Genetically Engineered Plum-Fruits

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