Genetic Engineering of Bacteria for Environmental Remediation of Mercury

Kiyono, Masako; Pan-Hou, Hidemitsu

doi:10.1248/jhs.52.199

Cited by 79 publications

(22 citation statements)

References 38 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…S1 contained a plasmid. In MRB, it has been clear that plasmid codes for one amongst genes coding for heavy metal resistance, and merA is the gene which codes for mercury reductase and therefore they are resistant to mercury and able to reduce Hg 2+ into Hg 0 (Kiyono & Pan-Hou, 2006). Mercury reductase activity of Bacillus sp.…”

Section: Resultsmentioning

confidence: 99%

“…However, in natural habitats, there are bacteria that might be able to resist mercury which are known as Mercury Resistant Bacteria (MRB). Such bacteria, possess enzymatic mechanism to reduce divalent mercury (Hg 2+ ) into volatile mercury of Hg 0 (Nascimento & Chartone-Souza, 2003) due to their possession of mercury resistant gene known as mer-operon, and merA is one of mer-operon groups that code for mercury reductase (Kiyono & Pan-Hou, 2006.). Usually, mer-operon gene is located within plasmid or transposon (Davis et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

MERCURY REDUCTASE ACTIVITY OF AN INDIGENOUS MERCURY RESISTANT BACTERIAL ISOLATE (Bacillus sp. S1) FROM KALIMAS-SURABAYA AS A POTENTIAL REDUCING AGENT FOR MERCURIAL ION (HG2+)

Zulaika

Sembiring

2015

KLS

View full text Add to dashboard Cite

Mercury reductase activity and Hg 2+ lowering capacity of a Mercury Resistant Bacteria (MRB) (Bacillus sp. S1) which was isolated from Kalimas River of Surabaya, Indonesia were studied. The activity was determined by Mercury Reductase Assay System (MRAS) in a solution mixture contained 50 mM PBS (pH ± 7.0), 0.5 mM EDTA, 200 µM MgSO4, 0.1% (v/v) ß-merchaptoethanol, 200 µM NADH2 and 25 mg/L HgCl 2 and one volume of crude extract incubated at room temperature for various interval period of time. Mercury reductase activity was measured spectrophotometrically at 340 nm. One unit of reductase activity was defined as one molar of oxidized NADH 2 produced per total cell per minute. Results of study showed that, the isolate could resist the concentration of HgCl 2 up to 11 mg/L. At 30 minute incubation period at room temperature, the highest mercury reductase activity and the Hg 2+ lowering capacity was found to be 0.006 unit/109cell and 1.48 mg/L/109cell/minute, respectively with the reduction efficiency of Hg 2+ to Hg 0 of 0.18% per minute. Therefore, it can be concluded that the Bacillus sp. S1 isolate could be assumed to be exellent mercury bioremediation agent since it was found to be highly mercury resistant and very efficient to reduce cationic mercury (Hg 2+ ) to elemental mercury (Hg 0 ).

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

MERCURY REDUCTASE ACTIVITY OF AN INDIGENOUS MERCURY RESISTANT BACTERIAL ISOLATE (Bacillus sp. S1) FROM KALIMAS-SURABAYA AS A POTENTIAL REDUCING AGENT FOR MERCURIAL ION (HG2+)

Zulaika

Sembiring

2015

KLS

View full text Add to dashboard Cite

show abstract

“…In response to the local geochemistry of contaminated environments, production of intracellular polyphosphate provides microorganisms with a means to sequester toxic ions within the cell cytosol as well as the regulation of gene(s) expressed in response to cellular stress (e.g., DNA repair, RNA polymerase sigma factor, and pH extremes) [55,[127][128][129][130][131]. The reactivity of cytosolic polyphosphates has been shown to facilitate intracellular sequestration of Cd, Cu, Hg, Pb, U, and Zn in genetically engineered bacterial strains as well as naturally occurring archaeal and bacterial strains [52,55,[132][133][134][135][136]. Electron microscopy analyses of cells exposed to these elements demonstrated intracellular localization with phosphate-rich granules, suggesting that contaminant sequestration may be achieved by polyphosphates and may protect sensitive cytosolic molecules from oxidative damage.…”

Section: Biological Approaches To Phosphate-mediated Immobilization Omentioning

confidence: 99%

Phosphate-Mediated Remediation of Metals and Radionuclides

Martinez

Beazley

Sobecky

2014

Advances in Ecology

View full text Add to dashboard Cite

Worldwide industrialization activities create vast amounts of organic and inorganic waste streams that frequently result in significant soil and groundwater contamination. Metals and radionuclides are of particular concern due to their mobility and longterm persistence in aquatic and terrestrial environments. As the global population increases, the demand for safe, contaminantfree soil and groundwater will increase as will the need for effective and inexpensive remediation strategies. Remediation strategies that include physical and chemical methods (i.e., abiotic) or biological activities have been shown to impede the migration of radionuclide and metal contaminants within soil and groundwater. However, abiotic remediation methods are often too costly owing to the quantities and volumes of soils and/or groundwater requiring treatment. The in situ sequestration of metals and radionuclides mediated by biological activities associated with microbial phosphorus metabolism is a promising and less costly addition to our existing remediation methods. This review highlights the current strategies for abiotic and microbial phosphatemediated techniques for uranium and metal remediation.

show abstract

“…The discharge of mercury into the environment as a result of industrial and anthropogenic sources has resulted in mercury pollution affecting ecosystems and human health and is problematic in both developed as well as developing countries [1][2][3]. Mercury is a potent neurotoxin [4,5] and exposure can lead to paresthesia and numbness in the fingers and toes, clumsiness, neurasthenia, fatigue, inability to concentrate, loss in vision and hearing impairment, coma followed by death, It gets accumulated in tissues worsening the condition [6].…”

mentioning

confidence: 99%

Cloning of merA Gene from Methylotenera Mobilis for Mercury Biotransformation

Porwal

Singh

2016

Indian J Microbiol

View full text Add to dashboard Cite

Mercury (Hg) is one of the most toxic heavy metal and is extremely harmful for the environment. The permissible limit of mercury in industrial effluents is 0.001 ppm, whereas there are various sites having very high levels of mercury contamination. In the present study, 10 different mercury (Hg) resistant bacterial strains were isolated from Ulhas Estuary, Mumbai (Hg concentration of 107 ppm). All the strains were subsequently grown on higher concentration of mercuric chloride (HgCl 2 ), one of the isolate (USP5) showed significant growth at high concentration of Hg (40 ppm) and 16S rRNA gene sequencing revealed the identity of the bacterium as Methylotenera mobilis, (Accession no. KT714144). The mer operon was isolated and cloned in E.coli and checked for its ability to tolerate higher concentration of Hg. It has shown growth up to 70 ppm of Hg, also presence of merA gene indicated its ability to detoxify Hg into less toxic volatile form. The atomic absorption spectrophotometry confirmed the ability of clone to efficiently detoxify 60-90 % of the Hg (10-70 ppm) within 48-72 h. This clone can be used for effective volatilization of Hg from contaminated areas.

show abstract

Genetic Engineering of Bacteria for Environmental Remediation of Mercury

Cited by 79 publications

References 38 publications

MERCURY REDUCTASE ACTIVITY OF AN INDIGENOUS MERCURY RESISTANT BACTERIAL ISOLATE (Bacillus sp. S1) FROM KALIMAS-SURABAYA AS A POTENTIAL REDUCING AGENT FOR MERCURIAL ION (HG2+)

MERCURY REDUCTASE ACTIVITY OF AN INDIGENOUS MERCURY RESISTANT BACTERIAL ISOLATE (Bacillus sp. S1) FROM KALIMAS-SURABAYA AS A POTENTIAL REDUCING AGENT FOR MERCURIAL ION (HG2+)

Phosphate-Mediated Remediation of Metals and Radionuclides

Cloning of merA Gene from Methylotenera Mobilis for Mercury Biotransformation

Contact Info

Product

Resources

About