Genetic Engineering in Papaya

Sirhindi, Geetika; Mushtaq, Ruqia; Kaur, Harsimran; Dogra, Neha; Kaushik, Shruti; Singh, Sheesh P.

doi:10.1016/b978-0-12-810439-2.00007-6

Cited by 3 publications

(6 citation statements)

References 92 publications

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“…gov/pz/portal.html#!info? alias=Org_Cpapaya [28] Agrobacterium-mediated transformation of somatic and zygotic embryos [29,30].…”

Section: Papaya 'Sun Up'mentioning

confidence: 99%

“…For papaya, genetic engineering has mostly involved biolistic-mediated transformation related to tolerance to the papaya ringspot virus, a Potyvirus that is quite possibly the most serious viral disease of papaya worldwide [29,30]. Nonetheless, Agrobacterium-mediated transformation of somatic and zygotic embryos has also been reported [30] (Table 1).…”

Section: Possibilities For Genome Editing In Coffee Cacao and Papayamentioning

confidence: 99%

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Chromosome Engineering in Tropical Cash Crops

Bolaños-Villegas

2020

Agronomy

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Tropical and subtropical crops such as coffee, cacao, and papaya are valuable commodities, and their consumption is a seemingly indispensable part of the daily lives of billions of people worldwide. Conventional breeding of these crops is long, and yields are threatened by global warming. Traditional chromosome engineering and new synthetic biology methods could be used to engineer new chromosomes, facilitate the transmission of wild traits to improve resistance to stress and disease in these crops, and hopefully boost yields. This review gives an overview of these approaches. The adoption of these approaches may contribute to the resilience of agricultural communities, lead to economic growth and secure the availability of key resources for generations to come.

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“…gov/pz/portal.html#!info? alias=Org_Cpapaya [28] Agrobacterium-mediated transformation of somatic and zygotic embryos [29,30].…”

Section: Papaya 'Sun Up'mentioning

confidence: 99%

Section: Possibilities For Genome Editing In Coffee Cacao and Papayamentioning

confidence: 99%

Chromosome Engineering in Tropical Cash Crops

Bolaños-Villegas

2020

Agronomy

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“…For papaya, genetic engineering has mostly involved biolistic-mediated transformation related to tolerance to the papaya ringspot virus, a single-strand, positive-sense RNA Potyvirus and quite possibly the most serious viral disease of papaya worldwide 36,37 . Nonetheless, Agrobacteriummediated transformation of somatic and zygotic embryos has also been reported 37 .…”

Section: Mechanisms Of Meiosis and Fertilitymentioning

confidence: 99%

Chromosome Engineering in Tropical Cash Crops

Bolaños-Villegas¹

2019

Preprint

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Background Tropical and subtropical crops such as coffee, cacao, and papaya are valuable commodities and its consumption is a seemingly indispensable part of the daily lives of billions of people across the world. Conventional breeding in these crops is lengthy and yields are threatened by runaway global warming. In this review we propose the application of chromosome engineering and synthetic biology principles in order to enhance synthesis of key metabolites, and transmission of wild traits for resistance to stress and disease. Conclusions It is hoped that the adoption of such technological approaches may enhance the resilience of agricultural communities, lead to economic growth and secure the availability of key resources for generations to come.

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“…Apomixis has been described as a process that involves deregulated proliferation of embryo-like structures due to changes in the activity of cell-cycle regulators and Polycomb-group proteins that control high-order chromatin architecture (Yang et al, 2010). However, no reliable cytological or molecular information exists on the molecular biology of embryo sac development in papaya, possibly because unlike in Arabidopsis, papaya embryo sacs are large (Thomson Foster, 1943) and cultivation cycles are much longer, 9 months or more (Geetika et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

“…Carica papaya Linn is a diploid species with male, female, or hermaphrodite flowers on the same plant. In nature, plants show male and female flowers on separate individuals (Geetika et al ., 2018). However, commercial papaya cultivars show hermaphrodite flowers in some plants and only female ones in others (Geetika et al ., 2018).…”

Section: Introductionmentioning

confidence: 99%

Embryo development inCarica papayaLinn

Acevedo-Benavides

Bolaños-Villegas

2021

Preprint

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Papaya (Carica papaya Linn.) is a tropical plant whose draft genome has been sequenced. Papaya produces large fruits rich in vitamins A and C and is an important cash crop in developing countries. Nonetheless, little is known about how the female gametophyte develops, how it is fertilized and how it develops into a mature seed containing an embryo and an endosperm. The Papaya female gametophyte displays a Polygonum-type architecture consisting of two synergid cells, an egg cell, a central cell, and three antipodal cells. We have reports of the presumed existence of varieties in which cross fertilization is bypassed and autonomous development of embryos occurs (e.g., apomixis). In this study, we analyzed the development of female gametophytes in a commercial Hawaiian parental line and in the presumed apomictic Costa Rican line L1. Samples were collected before and after anthesis to compare the overall structure, size and transcriptional patterns of several genes that may be involved in egg and endosperm cell fate and proliferation. These genes were the putative papaya homologs of ARGONAUTE9 (AGO9), MEDEA (MEA), RETINOBLASTOMA RELATED-1 (RBR1), and SLOW WALKER-1 (SWA1). Our results suggest that its feasibly to identify structural features of Polygonum-type development, and that in bagged female flowers of line L1 we might have observed autonomous development of embryo-like structures. Possible downregulation of papaya homologs for AGO9, MEA, RBR1 and SWA1 was observed in embryo sacs from line L1 before and after anthesis, which may suggest a link between suspected apomixis and transcriptional downregulation of genes for RNA-directed DNA methylation, histone remodelers, and rRNA processing. Most notably, the large size of the papaya embryo sac suggests that it could be a cytological alternative to Arabidopsis thaliana for study. Significant variation in embryo sac size was observed between the varieties under study, suggesting wide differences in the genetic regulation of anatomical features.

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Genetic Engineering in Papaya

Cited by 3 publications

References 92 publications

Chromosome Engineering in Tropical Cash Crops

Chromosome Engineering in Tropical Cash Crops

Chromosome Engineering in Tropical Cash Crops

Embryo development inCarica papayaLinn

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