Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives

Dong, Oliver Xiaoou; Ronald, Pamela C.

doi:10.1104/pp.18.01224

Cited by 211 publications

(150 citation statements)

References 162 publications

(187 reference statements)

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“…In contrast to R genes, there are also susceptibility (S) genes in plants that promote and facilitate the proliferation of any disease or pathogen attack. These genes negatively regulate the plant resistance against disease and usually include sugar transporters, i.e., SWEET gene family that transport sugar out of the cell and made available to pathogens ( 17 ). Similarly, some members of the NAC gene family are also used as target by the host for their multiplication ( 18 ).…”

Section: Genetics and Genomics Of Wheat To Combat Pstmentioning

confidence: 99%

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

et al. 2020

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Puccinia striiformis (Pst) is a devastating biotrophic fungal pathogen that causes wheat stripe rust. It usually loves cool and moist places and can cause 100% crop yield losses in a single field when ideal conditions for disease incidence prevails. Billions of dollars are lost due to fungicide application to reduce stripe rust damage worldwide. Pst is a macrocyclic, heteroecious fungus that requires primary (wheat or grasses) as well as secondary host ( Berberis or Mahonia spp.) for completion of life cycle. In this review, we have summarized the knowledge about pathogen life cycle, genes responsible for stripe rust resistance, and susceptibility in wheat. In the end, we discussed the importance of conventional and modern breeding tools for the development of Pst -resistant wheat varieties. According to our findings, genetic engineering and genome editing are less explored tools for the development of Pst -resistant wheat varieties; hence, we highlighted the putative use of advanced genome-modifying tools, i.e., base editing and prime editing, for the development of Pst -resistant wheat.

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Section: Genetics and Genomics Of Wheat To Combat Pstmentioning

confidence: 99%

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

et al. 2020

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“…The biological significance of the vast majority of these receptors remains elusive, and their underlying mechanism of ligand perception remains poorly understood. Understanding how cell-surface receptors with different ECDs perceive ligands will provide a foundation for engineering broad-spectrum resistance into crop plants ( 158 , 159 ). Further, our understanding of how RLCKs coordinate their association with different receptors and facilitate distinct signaling outputs is a key challenge for the future.…”

Section: Cell-surface Immunitymentioning

confidence: 99%

A molecular roadmap to the plant immune system

Bentham¹,

Concepcion²,

Mukhi³

et al. 2020

Journal of Biological Chemistry

102

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Plant diseases caused by pathogens and pests are a constant threat to global food security. Direct crop losses, and the measures used to control disease (e.g. application of pesticides), have significant agricultural, economic and societal impacts. Therefore, it is essential we understand the molecular mechanisms of the plant immune system, a system which allows plants to resist attack from a wide variety of organisms ranging from viruses to insects. Here, we provide a roadmap to plant immunity, with a focus on cell-surface and intracellular immune receptors. We describe how these receptors perceive signatures of pathogens and pests and initiate immune pathways. We merge existing concepts with new insights gained from recent breakthroughs on the structure and function of plant immune receptors, which have generated a shift in our understanding of cell-surface and intracellular immunity and the interplay between the two. Finally, we use our current understanding of the plant immunity as context to discuss the potential of engineering the plant immune system with the aim of bolstering plant defences against disease.

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“…Technically, the RNAi system is naturally present in crops, which means we only need to harness this machinery to target pathogenic RNAs via transformation of a short, artificial miRNA with homologies to the virus genome. Popular targets are, for example, viral coat proteins or replicases (Dong and Ronald, 2019). Also, stable constitutive expression of the CRISPR/Cas system can target viral genomes by suitable sgRNAs, thereby establishing a novel immune system (Ji et al, 2015;Zhang et al, 2018a;Mushtaq et al, 2020).…”

Section: Transgenic Approaches Deploying Crispr/cas To Increase Resismentioning

confidence: 99%

Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors

Schenke

Cai

2020

iScience

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Current crop production systems are prone to increasing pathogen pressure. Fundamental understanding of molecular plant-pathogen interactions, the availability of crop and pathogen genomic information, as well as emerging genome editing permits a novel approach for breeding of crop disease resistance. We describe here strategies to identify new targets for resistance breeding with focus on interruption of the compatible plant-pathogen interaction by CRISPR/ Cas-mediated genome editing. Basically, crop genome editing can be applied in several ways to achieve this goal. The most common approach focuses on the ''simple'' knockout by non-homologous end joining repair of plant susceptibility factors required for efficient host colonization. However, genome rewriting via homology-directed repair or base editing can also prevent host manipulation by changing the targets of pathogen-derived effectors or molecules beyond recognition, which also decreases plant susceptibility. We conclude that genome editing by CRISPR/Cas will become increasingly indispensable to generate in relatively short time beneficial resistance traits in crops to meet upcoming challenges.

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Genetic Engineering for Disease Resistance in Plants: Recent Progress and Future Perspectives

Cited by 211 publications

References 162 publications

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

Role of Genetics, Genomics, and Breeding Approaches to Combat Stripe Rust of Wheat

A molecular roadmap to the plant immune system

Applications of CRISPR/Cas to Improve Crop Disease Resistance: Beyond Inactivation of Susceptibility Factors

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