Genetic Engineering Approaches for Enhanced Production of Biodiesel Fuel from Microalgae

Roessler, Paul G.; Brown, Lewis M.; Dünahay, Terri G.; Heacox, D.A.; Jarvis, Erin; Schneider, Jan; Talbot, S.; Zeiler, Kathryn

doi:10.1021/bk-1994-0566.ch013

Cited by 75 publications

(40 citation statements)

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“…Spolaore et al [6] evaluated that microalgae can provide several different types of renewable biofuels. Many researchers reported that biodiesel was derived from microalgal oil [11,12,13,17] . The transesterification of used oil produced biodiesel by, using an alkaline catalyst, KOH [25] .…”

Section: Resultsmentioning

confidence: 99%

“…Microalgae can provide several different types of renewable biofuels. These include methane produced by anaerobic digestion of the algal biomass [6] biodiesel derived from microalgal oil [11,12,13] and photobiologically produced biohydrogen [14,15] . The idea of using microalgae as a source of fuel is not new [16,17] but it is now being taken seriously because of the escalating price of petroleum and, more significantly, the emerging concern about global warming that is associated with burning fossil fuels [18] .…”

Section: Introductionmentioning

confidence: 99%

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Biodiesel Fuel Production from Algae as Renewable Energy

Hossain¹,

Salleh²,

Boyce³

et al. 2008

American J. of Biochemistry and Biotechnology

424

146

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Biodiesel is biodegradable, less CO 2 and NO x emissions. Continuous use of petroleum sourced fuels is now widely recognized as unsustainable because of depleting supplies and the contribution of these fuels to the accumulation of carbon dioxide in the environment. Renewable, carbon neutral, transport fuels are necessary for environmental and economic sustainability. Algae have emerged as one of the most promising sources for biodiesel production. It can be inferred that algae grown in CO 2 -enriched air can be converted to oily substances. Such an approach can contribute to solve major problems of air pollution resulting from CO 2 evolution and future crisis due to a shortage of energy sources. This study was undertaken to know the proper transesterification, amount of biodiesel production (ester) and physical properties of biodiesel. In this study we used common species Oedogonium and Spirogyra to compare the amount of biodiesel production. Algal oil and biodiesel (ester) production was higher in Oedogonium than Spirogyra sp. However, biomass (after oil extraction) was higher in Spirogyra than Oedogonium sp. Sediments (glycerine, water and pigments) was higher in Spirogyra than Oedogonium sp. There was no difference of pH between Spirogyra and Oedogonium sp. These results indicate that biodiesel can be produced from both species and Oedogonium is better source than Spirogyra sp.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Biodiesel Fuel Production from Algae as Renewable Energy

Hossain¹,

Salleh²,

Boyce³

et al. 2008

American J. of Biochemistry and Biotechnology

424

146

View full text Add to dashboard Cite

show abstract

“…In algae, for example, the complete pathway from carbon dioxide fixation to TAG synthesis and sequestration takes place within a single cell, whereas the synthesis and accumulation (1) Cytosolic glycerol-3-phosphate acyl transferase, (2) lyso-phosphatidic acid acyl transferase, (3) phosphatidic acid phosphatase, and (4) diacylglycerol acyl transferase. Adapted from Roessler et al, 1994b. of TAG only occur in special tissues or organs (e.g. seeds or fruits) of oil crop plants.…”

Section: Comparison Of Lipid Metabolism In Algae and Higher Plantsmentioning

confidence: 99%

Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances

et al. 2008

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SummaryMicroalgae represent an exceptionally diverse but highly specialized group of micro-organisms adapted to various ecological habitats. Many microalgae have the ability to produce substantial amounts (e.g. 20-50% dry cell weight) of triacylglycerols (TAG) as a storage lipid under photo-oxidative stress or other adverse environmental conditions. Fatty acids, the building blocks for TAGs and all other cellular lipids, are synthesized in the chloroplast using a single set of enzymes, of which acetyl CoA carboxylase (ACCase) is key in regulating fatty acid synthesis rates. However, the expression of genes involved in fatty acid synthesis is poorly understood in microalgae. Synthesis and sequestration of TAG into cytosolic lipid bodies appear to be a protective mechanism by which algal cells cope with stress conditions, but little is known about regulation of TAG formation at the molecular and cellular level. While the concept of using microalgae as an alternative and renewable source of lipid-rich biomass feedstock for biofuels has been explored over the past few decades, a scalable, commercially viable system has yet to emerge. Today, the production of algal oil is primarily confined to high-value specialty oils with nutritional value, rather than commodity oils for biofuel. This review provides a brief summary of the current knowledge on oleaginous algae and their fatty acid and TAG biosynthesis, algal model systems and genomic approaches to a better understanding of TAG production, and a historical perspective and path forward for microalgae-based biofuel research and commercialization.

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“…5 represents the process of biodiesel production. Under optimal condition, green algae could double its biomass in a day with approximately 50% of lipid content [15,16]. The high-density biomass leads to an increase in the biodiesel production [13].…”

Section: Triglycerides+methanol+koh=glycerol+methyl Esters (Biodiesel)mentioning

confidence: 99%

An Insight on Algal Cell Disruption for Biodiesel Production

Aarthy

Smita

Turkar

et al. 2018

Asian J Pharm Clin Res

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Objective: This review article deals with the effect that various cell disruption techniques have on the efficiency of lipid extraction. We have reviewed existing algal cell disruption techniques that aid the biodiesel production process.Methods: Current rise in demand for energy has led the researcher to focus on the production of sustainable fuels, among which biodiesel has received greater attention. This is due to its larger lipid content, higher growth rate, larger biomass production, and lower land use. Extraction of lipid from algae (micro and macro) for the production of biodiesel involves numerous downstream processing steps, of which cell wall disruption is a crucial step. Bead milling, high-pressure homogenization, ultra-sonication, freeze-drying, acid treatment, and enzymatic lysis are some methods of cell disruption. The cell disruption technique needs to be optimized based on the structure and biochemical composition of algae. Result:The lipid extraction efficiency varies depending on the algal species and the cell disruption technique used. Conclusion:In-depth research and development of new techniques are required to further enhance the cell disruption of the algal cell wall for the enhanced recovery of lipids. In addition, the operating costs and energy consumption should also be optimized for the cost-effective recovery.

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Genetic Engineering Approaches for Enhanced Production of Biodiesel Fuel from Microalgae

Cited by 75 publications

References 0 publications

Biodiesel Fuel Production from Algae as Renewable Energy

Biodiesel Fuel Production from Algae as Renewable Energy

Microalgal triacylglycerols as feedstocks for biofuel production: perspectives and advances

An Insight on Algal Cell Disruption for Biodiesel Production

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