Genetic elimination of dengue vector mosquitoes

Valdez, Megan R. Wise de; Nimmo, Derric; Betz, John; Gong, Hui; James, Anthony A.; Alphey, Luke; Black, William C.

doi:10.1073/pnas.1019295108

Cited by 214 publications

(183 citation statements)

References 26 publications

(29 reference statements)

Supporting

Mentioning

175

Contrasting

Unclassified

Order By: Relevance

“…[10][11][12] Much progress has been made on genetic manipulation of Ae. aegypti, [13][14][15][16] but fewer resources have been allocated to understand ecologic aspects of this mosquito that could affect the efficacy of transgenic approaches. One strain of Ae.…”

Section: Introductionmentioning

confidence: 99%

Regulation of Aedes aegypti Population Dynamics in Field Systems: Quantifying Direct and Delayed Density Dependence

Walsh¹,

Aguilar²,

Facchinelli³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

Abstract. Transgenic strains of Aedes aegypti have been engineered to help control transmission of dengue virus. Although resources have been invested in developing the strains, we lack data on the ecology of mosquitoes that could impact the success of this approach. Although studies of intra-specific competition have been conducted using Ae. aegypti larvae, none of these studies examine mixed age cohorts at densities that occur in the field, with natural nutrient levels. Experiments were conducted in Mexico to determine the impact of direct and delayed density dependence on Ae. aegypti populations. Natural water, food, and larval densities were used to estimate the impacts of density dependence on larval survival, development, and adult body size. Direct and delayed density-dependent factors had a significant impact on larval survival, larval development, and adult body size. These results indicate that control methods attempting to reduce mosquito populations may be counteracted by density-dependent population regulation.

show abstract

Section: Introductionmentioning

confidence: 99%

Regulation of Aedes aegypti Population Dynamics in Field Systems: Quantifying Direct and Delayed Density Dependence

Walsh¹,

Aguilar²,

Facchinelli³

et al. 2013

The American Journal of Tropical Medicine and Hygiene

View full text Add to dashboard Cite

show abstract

“…One important outcome is dominant female‐specific lethality, alternatively described as male‐selecting constructs (Heinrich & Scott, 2000; Thomas et al ., 2000; Fu et al ., 2007, 2010; Wise de Valdez et al ., 2011; Ant et al ., 2012; Labbé et al ., 2012; Jin et al ., 2013; Tan et al ., 2013) (Fig. 1B).…”

Section: Sterile Insect Methodsmentioning

confidence: 99%

“…Where the juvenile stage of a pest insect causes damage to plants, such a late‐acting trait is less attractive. Variations also include tissue‐specific expression to render female mosquitoes flightless and therefore unable to feed or mate (Fu et al ., 2010; Wise de Valdez et al ., 2011; Labbé et al ., 2012). …”

Section: Sterile Insect Methodsmentioning

confidence: 99%

Genetics‐based methods for agricultural insect pest management

Alphey

Bonsall

2017

Agri and Forest Entomology

View full text Add to dashboard Cite

The sterile insect technique is an area‐wide pest control method that reduces agricultural pest populations by releasing mass‐reared sterile insects, which then compete for mates with wild insects. Contemporary genetics‐based technologies use insects that are homozygous for a repressible dominant lethal genetic construct rather than being sterilized by irradiation.Engineered strains of agricultural pest species, including moths such as the diamondback moth Plutella xylostella and fruit flies such as the Mediterranean fruit fly Ceratitis capitata, have been developed with lethality that only operates on females.Transgenic crops expressing insecticidal toxins are widely used; the economic benefits of these crops would be lost if toxin resistance spread through the pest population. The primary resistance management method is a high‐dose/refuge strategy, requiring toxin‐free crops as refuges near the insecticidal crops, as well as toxin doses sufficiently high to kill wild‐type insects and insects heterozygous for a resistance allele.Mass‐release of toxin‐sensitive engineered males (carrying female‐lethal genes), as well as suppressing populations, could substantially delay or reverse the spread of resistance. These transgenic insect technologies could form an effective resistance management strategy.We outline some policy considerations for taking genetic insect control systems through to field implementation.

show abstract

“…In one recent example of such an effect, a transgenic line of Ae. aegypti was shown to be able to suppress stable laboratory populations through weekly releases of effectively sterile males within 10-20 weeks (de Valdez et al 2011), but this same strain failed to achieve elimination in large outdoor field cages in southern Mexico with 17 weeks of releases (Facchinelli et al 2013).…”

Section: Discussionmentioning

confidence: 99%