Genetic Effects of LPIN1 Polymorphisms on Milk Production Traits in Dairy Cattle

Han, Bo; Yuán, Yǒulù; Liang, Rongrui; Li, Yanhua; Lin, Liu; Sun, Dongxiao

doi:10.3390/genes10040265

Cited by 26 publications

(18 citation statements)

References 30 publications

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“…On the other hand, missense mutation might be a candidate for production traits in cattle, there is a superior correlation between the gene polymorphisms and productive traits (26) , also, the missense mutations could be lead to a change in the protein structure (27) , this is consistent with the results of the study (Fig. 3), as the protein structure differed in all four haplotypes.…”

Section: Cont Table (2) Type Of Mutations In the Cyp19 Gene In Holstein Bulls In Iraqsupporting

confidence: 88%

Genetic Profiling of CYP19 Gene Haplotype in Holstein Bulls

Hassan Nima Habib,

Khalaf A.H.Al-Rishdy,

Murthda F.AL-Hellou

2021

Indian Journal of Forensic Medicine & Toxicology

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The CYP19 gene is a protein-coding gene that stimulates the transmutation of androgens to estrogens, aromatase activity is related to haplotypes of the CYP19 gene, therefore this study aimed to detect haplotypes of the CYP19 gene in the Holstein bulls. This study, conducted from October 2018 to June 2019. Forty-two Bulls, aged between 4-6 years old were used, which belonged to local farmers in Basra city, south of Iraq. The DNA was extracted from semen, PCR amplification was carried out to acquire the DNA sequence. The sequences were analyzed by using some Bioinformatics tools. The results showed four haplotypes in the CYP19 gene as a result of silent and missense mutations in comparison with the same gene in GenBank, all the haplotypes are recorded in GenBank, DDBJ and EMBL. These findings call for further studies to reveal the relationship of CYP19 gene haplotypes and semen characteristics to uncover bulls' fertility.

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Section: Cont Table (2) Type Of Mutations In the Cyp19 Gene In Holstein Bulls In Iraqsupporting

confidence: 88%

Genetic Profiling of CYP19 Gene Haplotype in Holstein Bulls

Hassan Nima Habib,

Khalaf A.H.Al-Rishdy,

Murthda F.AL-Hellou

2021

Indian Journal of Forensic Medicine & Toxicology

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“…A study has revealed that the use of available loci improved the accuracy of genomic prediction in dairy cattle [19], which suggests that loci that are associated with milk production traits could be used in genomic selection programs that are aimed at accelerating genetic progress of dairy cattle. Studies have shown that the SNPs in the candidate genes significantly influenced the milk yield and composition in Holsteins [20,21,22,23,24,25]. In this study, we identified ten SNPs in NUCB2 and found that the SNPs and haplotypes of this gene were significantly associated with milk production traits.…”

Section: Discussionmentioning

confidence: 68%

Single Nucleotide Polymorphisms of NUCB2 and Their Genetic Associations with Milk Production Traits in Dairy Cows

Han

Yuán

et al. 2019

Genes

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We previously used the RNA sequencing technique to detect the hepatic transcriptome of Chinese Holstein cows among the dry period, early lactation, and peak of lactation, and implied that the nucleobindin 2 (NUCB2) gene might be associated with milk production traits due to its expression being significantly increased in early lactation or peak of lactation as compared to dry period (q value < 0.05). Hence, in this study, we detected the single nucleotide polymorphisms (SNPs) of NUCB2 and analyzed their genetic associations with milk yield, fat yield, fat percentage, protein yield, and protein percentage. We re-sequenced the entire coding and 2000 bp of 5′ and 3′ flanking regions of NUCB2 by pooled sequencing, and identified ten SNPs, including one in 5′ flanking region, two in 3′ untranslated region (UTR), and seven in 3′ flanking region. The single-SNP association analysis results showed that the ten SNPs were significantly associated with milk yield, fat yield, fat percentage, protein yield, or protein percentage in the first or second lactation (p values <= 1 × 10−4 and 0.05). In addition, we estimated the linkage disequilibrium (LD) of the ten SNPs by Haploview 4.2, and found that the SNPs were highly linked in one haplotype block (D′ = 0.98–1.00), and the block was also significantly associated with at least one milk traits in the two lactations (p values: 0.0002–0.047). Further, we predicted the changes of transcription factor binding sites (TFBSs) that are caused by the SNPs in the 5′ flanking region of NUCB2, and considered that g.35735477C>T might affect the expression of NUCB2 by changing the TFBSs for ETS transcription factor 3 (ELF3), caudal type homeobox 2 (CDX2), mammalian C-type LTR TATA box (VTATA), nuclear factor of activated T-cells (NFAT), and v-ets erythroblastosis virus E26 oncogene homolog (ERG) (matrix similarity threshold, MST > 0.85). However, the further study should be performed to verify the regulatory mechanisms of NUCB2 and its polymorphisms on milk traits. Our findings first revealed the genetic effects of NUCB2 on the milk traits in dairy cows, and suggested that the significant SNPs could be used in genomic selection to improve the accuracy of selection for dairy cattle breeding.

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“…Genomic selection is widely used in dairy cattle breeding, and the development of efficient SNP markers can improve the accuracy of the selection. Studies have shown that the SNPs in the functional genes significantly influenced the milk production traits in dairy cattle [37–40]. Thus, the SNPs with large genetic effects on milk traits could be used as markers to increase the selection efficiency in specific dairy cattle populations.…”

Section: Discussionmentioning

confidence: 99%

Identification of single nucleotide polymorphisms of PIK3R1 and DUSP1 genes and their genetic associations with milk production traits in dairy cows

Han

Yuán

Shi

et al. 2019

J Animal Sci Biotechnol

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BackgroundPreviously, phosphoinositide-3-kinase regulatory subunit 1 (PIK3R1) and dual specificity phosphatase 1 (DUSP1) were identified as promising candidate genes for milk production traits due to their being differentially expressed between the dry period and the peak of lactation in livers of dairy cows. Hence, in this study, the single nucleotide polymorphisms (SNPs) of PIK3R1 and DUSP1 genes were identified and their genetic associations with milk yield, fat yield, fat percentage, protein yield, and protein percentage, were investigated using 1067 Chinese Holstein cows from 40 sire families.ResultsBy re-sequencing the entire coding region and 2000 bp of the 5′ and 3′ flanking regions of the two genes, one SNP in the 5′ untranslated region (UTR), three in the 3′ UTR, and two in the 3′ flanking region of PIK3R1 were identified, and one in the 5′ flanking region, one in the 3′ UTR, and two in the 3′ flanking region of DUSP1 were found. Subsequent single-locus association analyses showed that five SNPs in PIK3R1, rs42590258, rs210389799, rs208819656, rs41255622, rs133655926, and rs211408208, and four SNPs in DUSP1, rs207593520, rs208460068, rs209154772, and rs210000760, were significantly associated with milk, fat and protein yields in the first or second lactation (P values ≤ 0.0001 and 0.0461). In addition, by the Haploview 4.2 software, the six and four SNPs in PIK3R1 and DUSP1 respectively formed one haplotype block, and the haplotype-based association analyses showed significant associations between their haplotype combinations and the milk traits in both two lactations (P values ≤ 0.0001 and 0.0364). One SNP, rs207593520(T/G), was predicted to alter the transcription factor binding sites (TFBSs) in the 5′ flanking region of DUSP1. Further, the dual-luciferase assay showed that the transcription activity of allele T in rs207593520 was significantly higher than that of allele G, suggesting the activation of transcriptional activity of DUSP1 gene by allele T of rs207593520. Thus, the rs207593520 SNP was highlighted as a potential causal mutation that should be further verified.ConclusionsWe demonstrated novel and significant genetic effects of the PIK3R1 and DUSP1 genes on milk production traits in dairy cows, and our findings provide information for use in dairy cattle breeding.

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Genetic Effects of LPIN1 Polymorphisms on Milk Production Traits in Dairy Cattle

Cited by 26 publications

References 30 publications

Genetic Profiling of CYP19 Gene Haplotype in Holstein Bulls

Genetic Profiling of CYP19 Gene Haplotype in Holstein Bulls

Single Nucleotide Polymorphisms of NUCB2 and Their Genetic Associations with Milk Production Traits in Dairy Cows

Identification of single nucleotide polymorphisms of PIK3R1 and DUSP1 genes and their genetic associations with milk production traits in dairy cows

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