Genetic editing for improvement of fruit and small fruit crops

Tikhonova, N. G.; Khlestkina, Elena K.

doi:10.31676/0235-2591-2019-4-10-15

Cited by 3 publications

(1 citation statement)

References 16 publications

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“…have created conditions for rapid and less labor-intensive improvement of fruit properties and the selection of pathogen and pest-resistant varieties [15]. Next-generation breeding methods for genome fruit and berry cultures editing are being intensively developed today [16].…”

Section: Introductionmentioning

confidence: 99%

Applications of CRISPR/Cas9 System in Vegetatively Propagated Fruit and Berry Crops

et al. 2021

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Fruit and berry crops, as well as grapes, are important parts of the human diet and, at the same time, significant objects of genetic, breeding, biochemical and nutritional research. Traditional approaches of crop research and improvement are now complemented by effective modern genetic technologies. In this review, we analyze and summarize the achievements in genome editing of fruit, berry crops and grapes. New approaches accelerate the improvement of genotypes for many groups of traits: plant resistance to unfavorable environmental factors, flowering and ripening time, plant architectonics, fruit shelf time and biochemical composition. Genome editing using the CRISPR/Cas9 system has been successfully tested on the most important vegetatively propagated fruit and berry crops (apple, pear, orange, kumquat, grapefruit, banana, strawberry and kiwi) and grapes. About 30 genes of these crops have been used as targets for the introduction of desired mutations using the CRISPR/Cas9 system. The most valuable results are the improvement of important agronomic traits. For 24 genes it has been shown that their knockout can result in the improvement of varieties. In addition, the review pays attention to the comparative analysis of the explant types of vegetatively propagated crops used for the delivery of editing genetic constructs, as well as the comparison of the editing efficiency depending on the variation of the objects used, delivery methods, etc. The article discusses the existing limitations that need to be overcome for a wider application of genomic editing in order to improve varieties of fruit and berry crops, as well as grapes.

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Section: Introductionmentioning

confidence: 99%

Applications of CRISPR/Cas9 System in Vegetatively Propagated Fruit and Berry Crops

et al. 2021

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<i>In vitro</i> regeneration of soybean (a review)

Беспалова

Ershova

Ukhatova

2021

Trudy po prikladnoy botanike, genetike i selektsii

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This is an overview of contemporary published works dedicated to the ability of soybean plants to regenerate in vitro and the techniques to achieve high regeneration rates, which is a necessary condition for the inclusion of soybean genotypes in genome editing programs. The main factors that determine the regenerative capacity of explants from various soybean accessions are considered. The greatest effect on the efficiency of regeneration is exerted by the conditions of in vitro culture initiation, type of explant, composition of the nutrient medium, shelf life of seeds, and genotypic characteristics of soybean accessions.

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Application of the CRISPR/Cas system for gene editing in ornamental crops

Rakhmangulov

2022

Biotehnologiâ i selekciâ rastenij

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Ornamental plants are widespread and popular all over the world. Floriculture industry is of significant economic importance for some countries. Favorable prospects for the development of industrial floriculture were also noted for Russia. This can be facilitated by CRISPR/Cas, a breakthrough method of editing genes responsible for economically valuable traits of plants, which allows bypassing the limitations of the potential intraspecific variability of plants and solving the problem of obtaining non-transgenic modified plants. This article analyzes the current status of ornamental crop breeding using the CRISPR/Cas genetic editing method. The articles were selected from the Scopus database. A search encompassing 50 most common ornamental crops yielded the total of 26 articles on genetic editing using the CRISPR/Cas system, in particular: 8 articles featuring petunia; 1 per each crop on chrysanthemum, kalanchoe, poinsettia and tobacco; 2 per each on dendrobium, gentian, lily and torenia, and 3 per each on phalaenopsis and ipomoea. The found articles were divided into three groups. The first group includes works devoted to studies of mechanisms of genes controlling useful traits, as well as the optimization of the CRISPR/Cas method for a particular crop. The second group unites works aimed at modifying color of flowers and leaves. The third group includes works on increasing the life span of a flower and obtaining double flowers. The review offers the works on the optimization of gene editing in representatives of the orchid family Orchidaceae Juss. Also, it notes the prospects of gene editing by the CRISPR/Cas system, which can accelerate qualitative improvements in breeding and raise it effectiveness, it being especially important in present conditions.

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Genetic editing for improvement of fruit and small fruit crops

Cited by 3 publications

References 16 publications

Applications of CRISPR/Cas9 System in Vegetatively Propagated Fruit and Berry Crops

Applications of CRISPR/Cas9 System in Vegetatively Propagated Fruit and Berry Crops

<i>In vitro</i> regeneration of soybean (a review)

Application of the CRISPR/Cas system for gene editing in ornamental crops

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