Genetic Diversity Within Lychee (Litchi Chinensis Sonn.) Based on Rapd Analysis

Anuntalabhochai, S.; Chundet, R.; Chiangda, J; Apavatjrut, Pimchai

doi:10.17660/actahortic.2002.575.27

Cited by 32 publications

(28 citation statements)

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“…This holds true even with separate components instead of using master mix for the PCR. Our results are in accordance with those of Anuntalabhochai et al (16), who reported that a high annealing temperature of 46 °C gave greater polymorphism, reproducibility, and resolution in RAPD. They concluded that RAPD is a useful procedure to differentiate between closely related and morphologically indistinct species.…”

Section: Discussionsupporting

confidence: 93%

Genetic characterization of paramphistomes of buffalo by HAT-RAPD analysis

Puttalakshmamma¹,

Ramani²,

Singh³

et al. 2014

Turk J Vet Anim Sci

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Section: Discussionsupporting

confidence: 93%

Genetic characterization of paramphistomes of buffalo by HAT-RAPD analysis

Puttalakshmamma¹,

Ramani²,

Singh³

et al. 2014

Turk J Vet Anim Sci

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“…In addition, from the same study, microsatellite markers linked to the specifically collagen-related genes were used to identify the responsible polymorphism; however, the result indicated a non-involvement in the pathogenesis of patellar luxation, which supported the conclusion of Chase et al [3] that patellar luxation is polygenic. Thus, the HAT-RAPD marker was chosen to identify the potential candidate polymorphisms accounting for the pathogenesis of patellar luxation, considering the ability of conventional RAPD to simultaneously screen several loci in the genome [12] and the high reproducibility of newly adapted HAT-RAPD [8] .…”

Section: Discussionmentioning

confidence: 99%

“…The amplifications followed the protocol of Anuntalabhochai et al [8] and Liu et al [9] . PCR was performed in a total volume of 25 µl containing: 1X reaction buffer (500 mM KCl, 15 mM MgCl 2 , 100 mM TrisHCl, 1 mg/ml BSA, and 100 mM (NH 4 ) 2 SO 4 ; RBC Bioscience, Taipei, Taiwan); 2 mM MgCl 2 (RBC Bioscience); 0.2 mM dNTP (Vivantis Technologies, Malaysia); 0.4 µM primers (Table 2) (Operon Technologies, Alameda CA, USA); 1 U Taq DNA polymerase (RBC Bioscience); 10 ng/ml genomic DNA; and deionized distilled water.…”

Section: Hat-rapd Analysismentioning

confidence: 99%

Detection of DNA Markers in Dogs with Patellar Luxation by High Annealing Temperature - Random Amplified Polymorphic DNA Analysis

Chomdej¹,

Kuensaen²,

Pradit³

et al. 2014

Kafkas Univ Vet Fak Derg

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SummaryPatellar luxation is one of the orthopaedic disorders found mostly in small-breed dogs. It can be inherited by the next generation, causing continuing problems in the dogs' health. However, if it can be detected, the affected dogs will not be selected for breeding, and hence the incidence will be less. In this study, the objective was to find a DNA marker representing dogs with patellar luxation which can be detected when first born. High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) technique was used to amplify 39 dog blood samples (16 unaffected and 23 affected). It was also used to develop the polymorphic fragments capable of distinguishing patellar-luxation-affected dogs from those unaffected. Three candidate fragments were sequenced and found to be parts from three different chromosomes (10, 36 and X) after comparison with the GenBank dog genome database using the BLAST algorithm. Association analysis was performed using a chi-square test. The results showed that the fragment (generated by the OPB05 primer) from chromosome 36 was potentially related to the two groups of dogs, with a P value of 0.042. This is the first finding of a gene which related to canine patellar luxation, and merits further investigation. Keywords: Patella luxation, Dog, HAT-RAPD, DNA marker Patella Çıkığı Olan Köpeklerde DNA Belirteçlerinin Yüksek Bağlanma Sıcaklıklı RAPD Analizi ile Belirlenmesi ÖzetPatellar luksasyon küçük ırk köpeklerde en yaygın gözlenen bozukluklardan birisidir. Sonraki nesillere aktarılmak suretiyle köpeklerde süregelen bir problem olabilir. Ancak tespit edilmesi durumunda etkilenmiş köpekler üretim amaçlı kullanılmaz ve böylece görülme sıklığı azaltılabilir. Bu çalışmada, patellar luksasyon ile doğan köpeklerin tespitinde kullanılmak amacıyla bir DNA markır bulmak amaçlanmıştır. High annealing temperature-random amplified polymorphic DNA (HAT-RAPD) tekniği ile 39 köpeğin (16 normal, 23 patellar luksasyonlu) kan örnekleri kullanıldı. Üç aday parçacık (fragment) sekanse edildi ve BLAST algoritması kullanılarak Den Bank köpek genomu ile karşılaştırıldığında bunların üç farklı kromozomdan (10, 36 ve X) bölümler olduğu tespit edildi. Asosiasyon analizi chi-kare testi kullanılarak yapıldı. Sonuçlar 36. kromozomdaki parçacığın (OPB05 primeri ile üretilen) potansiyel olarak iki grup köpek ile ilgili olduğunu ortaya koydu (P=0.042). Bu çalışma köpek patellar luksasyon ile ilgili bir gene ilişkin ilk çalışma olup gelecek araştırmalara ihtiyaç vardır.

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“…This 20 μl solution was then amplified in a Perkin-Elemer thermal cycler (Gene Amp PCR system 2400) using the following cycling profile: 95°C for 2 minutes, followed by 35 cycles of denaturing at 95°C for 30 seconds, annealing at 46-55°C for 30 seconds, and extension at 72°C for 45 seconds, followed by a final 5 minutes at 72°C. This cycling profile has been shown to increase band reproducibility due to the increased annealing temperature which limits imperfect template matches [20]. After the thermal cycling program had been completed, the amplification samples were stored at 4°C prior to a standard agarose gel electrophoresis run.…”

Section: Materials and Methodologymentioning

confidence: 99%

Phylogenetic Diversity of Ficus Species Using HAT-RAPD Markers as a Measure of Genomic Polymorphism

Anuntalabhochai¹,

Phromthep²,

Sitthiphrom³

et al. 2008

TOASJ

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Abstract:To create a molecular marker characterization for twenty species of Ficus, eight decamer primers were used to randomly amplify these species genomic DNA producing a total of 172 distinct polymorphic band patterns. One band was chosen to be converted into the more robust sequence characterized amplified region (SCAR) marker format to provide a unique molecular marker characterization for the variety of Ficus hirta. This technique for species identification and characterization provides a morphologically independent test to verify relatedness and provide species information particularly for cases where such identification was previously untenable such as in the case of morphologically indistinguishable plant cuttings.

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Genetic Diversity Within Lychee (Litchi Chinensis Sonn.) Based on Rapd Analysis

Cited by 32 publications

References 0 publications

Genetic characterization of paramphistomes of buffalo by HAT-RAPD analysis

Genetic characterization of paramphistomes of buffalo by HAT-RAPD analysis

Detection of DNA Markers in Dogs with Patellar Luxation by High Annealing Temperature - Random Amplified Polymorphic DNA Analysis

Phylogenetic Diversity of Ficus Species Using HAT-RAPD Markers as a Measure of Genomic Polymorphism

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