Genetic diversity within landraces of barley (Hordeum vulgare L.) and its implications on germplasm collection and utilization

Abebe, Tiegist Dejene; Abate, Alemayehu; Léon, Jens

doi:10.1007/s10722-023-01549-0

Cited by 5 publications

(4 citation statements)

References 42 publications

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“…The results of the principal coordinate analysis, UPGMA dendrogram, and STRUCTURE analysis proved that there was high gene flow among collection regions, indicating less contribution to genetic variability. The present study showed a certain match with the previous findings [ 13 , 36 ].…”

Section: Discussionsupporting

confidence: 93%

“…The present situation confirms that genetic distance is unrelated to geographic distance. Earlier studies on barley revealed comparable results [13,36,65,66].…”

Section: Plos Onementioning

confidence: 56%

“…It is an ideal marker for assessing genetic diversity, identifying appropriate parental lines for breeding, and managing germplasm collections [35]. Several genetic diversity studies were performed in different germplasm accessions in Ethiopia using SSR [13,[36][37][38][39]. However, these and other studies generated information from a small proportion of the available germplasm.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of genetic diversity and population structure of some Ethiopian barley (Hordeum vulgare L.) accessions using SSR markers

Zewodu,

Mohammed,

Shiferaw

2024

PLoS ONE

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Understanding the genetic diversity of existing genetic resources at the DNA level is an effective approach for germplasm conservation and utilization in breeding programs. However, the patterns of genetic diversity and population structure remain poorly characterized, making germplasm conservation and breeding efforts difficult to succeed. Thus, this study is aimed to evaluate the genetic diversity and population structure of 49 barley accessions collected from different geographic origins in Ethiopia. Twelve SSR markers were used to analyze all accessions and a total of 61 alleles were found, with a mean of 5.08 alleles per locus. The analysis pointed out the existence of moderate to high values of polymorphic information content ranging from 0.39 to 0.91 and the mean Shannon diversity index(I) was 1.25, indicating that they were highly informative markers. The highest Euclidean distance (1.32) was computed between accession 9950 and two accessions (247011 and 9949), while the lowest Euclidean distance (0.00) was estimated between accessions 243191 and 243192. The result of molecular variance analysis revealed that the highest variation was found among accessions (47) relative to within accessions (44) and among geographic origins (9). Cluster analysis grouped the 49 barley accessions into three major clusters regardless of their geographic origin which could be due to the presence of considerable gene flow (2.72). The result of the STRUCTURE analysis was consistent with neighbor-joining clustering and principal coordinate analysis. Generally, this study concluded that the variation among accessions was more important than the difference in geographical regions to develop an appropriate conservation strategy and for parental selection to use in breeding programs. This information will be helpful for barley conservation and breeding, and it may speed up the development of new competing barley varieties.

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Section: Discussionsupporting

confidence: 93%

“…The present situation confirms that genetic distance is unrelated to geographic distance. Earlier studies on barley revealed comparable results [13,36,65,66].…”

Section: Plos Onementioning

confidence: 56%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Analysis of genetic diversity and population structure of some Ethiopian barley (Hordeum vulgare L.) accessions using SSR markers

Zewodu,

Mohammed,

Shiferaw

2024

PLoS ONE

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“…SSRs are tenderly repeated nucleotide motifs 1-6 bp (Mukhopadhyay et al, 2023;Yang et al, 2023) which are co-dominant, reproducible, highly e cient, detect high level of polymorphism and are evenly distributed (Sandra et al, 2023). SSRs provided an abundant class of polymorphisms in crops including soybean (Kumar et al, 2022), wheat (Lue et al, 2023), barley (Abebe et al, 2023), rice (Bhatia et al, 2022, Al daej et al, 2023 and maize (Bhatia et al, 2022, Pandita et al, 2023. In this connection a variety of SSR markers have been developed to improve various plant attributes as well as increase the economic rate of Brassica varieties.…”

Section: Introductionmentioning

confidence: 99%

Molecular Characterization of Brassica Species using SSR Markers

Khan,

Jadoon,

Ahmad

et al. 2023

Preprint

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Genetic diversity and relationship among F8-populations of brassica species: B. napus, B. rapa and B. juncea were carried out on eight parental-lines along with their 38 introgressed populations using SSR markers to cover the diversity present in them as broadly as possible. Diversity analysis based on three neutral markers formed four distinct clusters with 1, 3, 19 and 22 genotypes, respectively. Based on these finding traits specific markers were employed which further formed six distinct clusters of selected plants with 3, 17, 2, 6, 3 and 14 genotypes. The cluster analysis identified genotypes with desirable biochemical contents i.e. plant 1 of Bnj43 had low glucosinolate, plant 3 of Bnr38 had high oleic acid content, plant 5 of Bnr38 had low linolenic acid, plant 1 of Bnr23 had low erucic acid while plant 2 of Bnr25 had high oil content and plant 2 of Bnj13 had high protein content. Moreover, the polymorphism information content (PIC) value of neutral as well as trait specific SSRs ranged from 0.5 to 0.96 and 0.45 to 0.95, respectively with an overall PIC value for all primers of 0.67. Considerable genetic diversity found among the F8-populations and their parents using SSR markers proved SSR analysis to be a useful tool. Moreover, based on the current findings, populations Bnj50F8, Bnr38F8, Bnj43F8, Bnj3F8 and Bnj25F8 are suggested to be used as source populations for transfer of desirable traits in future Brassica improvement programs.

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Phenotypic characterization of two-row barley (Hordeum vulgare L. ssp. vulgare) germplasm conserved in Osman Tosun Genebank of Türkiye by multivariate analysis model

Benlioglu,

Bilir,

Akdogan

et al. 2024

Genet Resour Crop Evol

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Barley is a crucial cereal crop with a diverse genetic heritage that includes landraces-traditionally cultivated variants that have adapted to varying agro-climatic conditions over centuries. Preserving barley landraces in genebanks is of utmost importance for safeguarding genetic diversity and aiding future breeding endeavors.This study aims an agro-morphological characterization of a selected collection of two row barley landraces conserved in the Osman Tosun Genebank. A total of 262 barley accessions were assessed in controlled field conditions to analyze various agro-morphological traits. The study unveiled notable disparities in the morphological traits of the genotypes, specifically in flag leaf anthocyanin, flag leaf sheath wax, spike shape, spike density, lemma color, grain color, lodging tendency, days to 50% flowering, plant height, spike length, spikelet quantity, and thousand grain weight, among other critical agronomical characteristics. To scrutinize the morphological disparities, the research utilized principal component analysis and ascertained that nearly 77.22% of the overall variance was accounted for by the first two principal components. The first four principal components accounted for 90.96% of the total variation, thus indicating significant genetic diversity among genotypes. The results of the cluster analysis revealed the presence of diverse genetic resources within the collection, with distinct groups of genotypes exhibiting similar traits being identified. The results of the agro-morphological characterization are a valuable resource for researchers and breeders, aiding them in making informed decisions regarding the management and application of barley landraces in breeding programs.

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Genetic diversity within landraces of barley (Hordeum vulgare L.) and its implications on germplasm collection and utilization

Cited by 5 publications

References 42 publications

Analysis of genetic diversity and population structure of some Ethiopian barley (Hordeum vulgare L.) accessions using SSR markers

Analysis of genetic diversity and population structure of some Ethiopian barley (Hordeum vulgare L.) accessions using SSR markers

Molecular Characterization of Brassica Species using SSR Markers

Phenotypic characterization of two-row barley (Hordeum vulgare L. ssp. vulgare) germplasm conserved in Osman Tosun Genebank of Türkiye by multivariate analysis model

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