Genetic diversity within Lablab purpureus and the application of gene-specific markers from a range of legume species

Abstract

Molecular markers have been used to study genetic diversity within a set of Lablab purpureus accessions collected from the southern states of India. Amplified fragment length polymorphism (AFLP) molecular marker studies using a total of 78 L. purpureus accessions with nine primer combinations showed there was very little genetic diversity within the L. purpureus accessions from the southern Indian germplasm collection as compared to a set of 15 accessions from other international germplasm collections that included African accessions. The set of 15 were selected from a random amplified length polymorphism (RAPD) marker study and chosen on the basis of widest genetic distance. Further molecular analysis with polymerase chain reaction (PCR) markers from 97 expressed sequence tag (EST) and gene-specific primer pairs, designed from a range of legume sequences, concurred with the AFLP analyses. Both of these approaches provide a wealth of markers for diversity and mapping studies. The 97 sequence-specific primer pairs tested in L. purpureus resulted in 70% amplification success, with 44% of primer pairs amplifying single bands and 10% double bands. Markers generated from these EST and genomic sequences provide useful cross-reference to comparative legume genomics that will potentially have long-term benefit to legume plant breeding.

Simple Sequence Repeat (SSR) Marker Assay-Based Genetic Diversity among Dolichos Bean (Lablab purpureus L. Sweet) Advanced Breeding Lines Differing for Productivity per se Traits

“…The average gene diversity was as low as 0.32 at SSR marker locus, KTD 250 and as high as 0.72 at SSR marker locus KTD 241 with an average of 0.52 among 16 genotypes. The average gene diversity in the present study is more than that reported in dolichos bean by Venkatesha et al, (2007) and (Kinmani et al, 2012), by Sarikamis et al, (2009) in common bean and by Gwag et al, (2006) in mung bean.…”

Molecular Diversity of Kenyan Lablab Bean (<i>Lablab purpureus</i> (L.) Sweet) Accessions Using Amplified Fragment Length Polymorphism Markers

“…The low diversity within the Kenyan L. purpureus accessions was also displayed in the principal coordinate analysis (PCoA) plot (Figure 2) with a tight cluster of points. A PCoA plot for L. purpureus accessions from UAS Bangalore and from Southern states of India also revealed a tight cluster of points [12]. The diversity however was increased by including accessions from Africa indicating greater genetic diversity across the continents.…”

“…Different marker systems have been used to identify genetic diversity in Lablab purpureus. These include simple sequence repeats [10,11], gene specific primers and expressed sequence tags (EST) [12]; Random Amplified Polymorphic DNA (RAPDs) [13][14][15][16] and Amplified fragment length polymorphism (AFLP) [12,[17][18][19]. AFLP markers are the choice markers for this study.…”

Microsatellite analysis of selected <i>Lablab purpureus</i> genotypes in Kenya

“…In comparable lablab studies using RAPDs and SRRs, Rai et al, (2010) (Wang et al, 2006). The low amplification of this study could be attributed to use of primers that were not specifically designed from lablab species, therefore primer sequence did not coincide with axon/intron regions from the genome (Venkatesha et al, 2007).…”

“…To date, no SSR markers for Lablab purpureus have been reported. However, the vast amount of markers available from related legume species can be applied in lablab studies considering the fact that mapping within the legume species provide evidence that these markers can be used in neglected species such as Lablab purpureus (Venkatesha et al, 2007;Zhu et al, 2005).…”