Genetic diversity within and among vegetative compatibility groups of Stereum sanguinolentum determined by arbitrary primed PCR

Abstract

Genetic variation within and among vegetative compatibility groups (VCGs) of Stereum sanguinolentum isolates was investigated with various geographical distances. DNA fingerprints were made using the M13 core sequence as a primer. A total of 113 isolates from 12 plots in Sweden, Finland and Lithuania were studied. Each VCG produced a discrete group of banding patterns. Among 20 isolates from the largest VCG, G1, the incidence of identical banding patterns was 24% within a sample plot, 8% among sample plots within a country, and 0% among countries. In the other 15 VCGs that comprised two and more isolates, corresponding percentages were 42%, 30% and 0%. Average band‐sharing indices (ABSIs) within VCGs decreased with increasing geographical distance: for isolates from VCG G1, ABSI was 91.3% within sample plots, 85.4% among sample plots within a country, and 79.2% among countries. Corresponding figures for the other 15 VCGs were 97.0%, 92.7% and 80.4%, respectively. Among VCGs similarities were significantly lower (P < 0.001) and the trend of decreasing similarity with larger geographical distances was less pronounced: ABSI within sample plots was 73.7%, among sample plots within countries 73.8%, and among countries 71.1%. However, the similarity of populations as compared within and between countries differed significantly (P < 0.001), suggesting geographical differentiation between S. sanguinolentum populations separated by 400 km and the Baltic sea. Calculations of GST clearly indicated geographical population subdivision within the large VCG but not among the total sample. In summary, the results show population structure of inbred lines within VCGs containing closely related strains in local populations and more distant relationships among populations.

Spread of Stereum sanguinolentum vegetative compatibility groups within a stand and within stems of Picea abies

“…Most suitable temperature range for infections of S. sanguinolentum lays between -8.3 and +5.0 °C (Kallio and Hallaksela 1979) and non-vegetation period wounds are more susceptible to establishment by the fungus as compared with vegetation period injuries (Etheridge 1969, Vasiliauskas andStenlid 1998b). It is known that VCGs of the fungus may be descendants from the same spore source (Ainsworth 1987) and, on the local scale, are composed from genetically identical or nearly identical individuals (Stenlid and Vasiliauskas 1998). However, no spatial clustering of the isolates from the same VCGs was observed in the stand, and a similar result has been reported in an earlier Swedish study (Vasiliauskas and Stenlid 1998a).…”

Genetic Diversity of Isolates of Glomus mosseae from Different Geographic Areas Detected by Vegetative Compatibility Testing and Biochemical and Molecular Analysis

“…Such tests, performed by scoring hyphal interactions directly, revealed the occurrence of isolates belonging to different vegetative compatibility groups (VCGs) within the same fungal species (9,10,13,20,21,29,33,44,45,54). Moreover, different experimental works reported that vegetative compatibility tests were often consistent with genetic and biochemical analyses and that genetic diversity was generally greater between isolates belonging to different VCGs (8,32,44,48).…”

An examination of genetic variation and disease spread in Inonotus tomentosus in the sub-boreal spruce zone of British Columbia.

“…In other words, isolates that have incompatible reactions are clearly genetically dissimilar, but when two isolates appear compatible it does not prove that they are clones, only that they are similar at the loci for compatibility (Hansen 1979). The results of this study are in agreement with other research that has shown multi-locus identification methods like RAPDs can further discriminate among genotypes identified by VC analysis (Jacobsen et al 1993;Stenlid and Vasiliauskas 1998). It is not known how many alleles or loci are involved in the somatic incompatibility system of I. tomentosus, but in most fungi it is a complicated multi allelic system (Leslie 1993;Adams and Roth 1967;Barret and Uscuplic 1971;Hansen 1979;Nauta and Hoekstra 1994;Saupe and Glass 1997).…”