Genetic diversity, virulence factors, and antimicrobial resistance of Listeria monocytogenes from food, livestock, and clinical samples between 2002 and 2019 in China

Anwar, Tanveer Muhammad; Pan, Hang; Chai, Wenqin; Ed-Dra, Abdelaziz; Fang, Weihuan; Li, Yan; Yue, Min

doi:10.1016/j.ijfoodmicro.2022.109572

Cited by 26 publications

(10 citation statements)

References 73 publications

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“…The samples included 756 boiled rice dishes, 91 boiled rice noodles, 105 fried rice noodles, 33 sticky rice rolls, 64 boiled sticky rice dishes, and 22 fried rice cakes. Quantitative detection of B. cereus in each sample was performed by using the direct plating method ( Liu et al, 2021 ; Yue et al, 2021 ; Anwar et al, 2022 ). Briefly, Twenty-five grams of each sample was suspended in 225 mL of PBS and subsequently homogenized for 2 mins.…”

Section: Methodsmentioning

confidence: 99%

Prevalence and antimicrobial-resistant characterization of Bacillus cereus isolated from ready-to-eat rice products in Eastern China

Chen

Zhang

et al. 2022

Front. Microbiol.

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Bacillus cereus is a major food-borne bacterial pathogen in the world, which can cause diarrhea and emetic syndrome. This study aimed to reveal the quantitative prevalence of B. cereus in ready-to-eat (RTE) rice products in Eastern China and to gain essential information on the characteristics of B. cereus isolates. A total of 91 out of the 1071 samples were positive for B. cereus. The contamination level of B. cereus in 0.5 % of RTE rice product samples outnumbered 103 CFU/g. The number of B. cereus attained 105−106 CFU/g in one sample. The distribution patterns of virulence genes in B. cereus isolates were identified. 84.6% of the B. cereus isolates had at least one enterotoxin or emetic toxin gene. The predominant pattern was XXV. 9.9% of isolates belonged to it and possessed one enterotoxin gene entFM. The occurrence rate of hblACD and nheABC was 36.3% and 47.3%, respectively. Antimicrobial susceptibility tests revealed a high resistance rate toward penicillin, and 23.1% of the isolates were multi-drug resistant. B. cereus isolates were genotyped by using ERIC-PCR. 89 genotypes were determined. The Hunter Gaston Discriminatory Index (HGDI) attained 0.9995. Relationships analysis revealed that Group A B. cereus isolates tended to carry hblA, hblC, hblD, nheA, nheB, and show resistance to penicillin/trimethoprim/sulfamethoxazole. This study was useful for updating the knowledge of the contamination status of B. cereus in RTE rice products in China.

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Section: Methodsmentioning

confidence: 99%

Prevalence and antimicrobial-resistant characterization of Bacillus cereus isolated from ready-to-eat rice products in Eastern China

Chen

Zhang

et al. 2022

Front. Microbiol.

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“…The MLST method has been shown to be highly discriminatory for L. monocytogenes typing (Anwar et al, 2022;Salcedo et al, 2003).…”

Section: Multi-locus Sequence Typing (Mlst)mentioning

confidence: 99%

“…Nevertheless, the MLST approach was one of the most commonly used (and it is still applied) typing methods for L. monocytogenes in assessing the genetic variation in the bacterial population clusters and in studying molecular epidemiology of listeriosis before the NGS era (Anwar et al, 2022;Pérez-Losada et al, 2013).…”

Section: Multi-locus Sequence Typing (Mlst)mentioning

confidence: 99%

“…A comparable discriminatory potential of the MLST and PFGE methods has also been described by other authors (Henri et al., 2016 ; Jadhav et al., 2012 ; Zhang et al., 2004 ), although there are also information that MLST is a more powerful molecular tool for differentiation of L. monocytogenes isolates (Mohan et al., 2021 ; Revazishvili et al., 2004 ) or vice versa (Ariza‐Miguel et al., 2015 ; Hilliard et al., 2018 ; Wang et al., 2012 ). Nevertheless, the MLST approach was one of the most commonly used (and it is still applied) typing methods for L. monocytogenes in assessing the genetic variation in the bacterial population clusters and in studying molecular epidemiology of listeriosis before the NGS era (Anwar et al., 2022 ; Pérez‐Losada et al., 2013 ).…”

Section: Molecular Typingmentioning

confidence: 99%

“…The MLST method has been shown to be highly discriminatory for L. monocytogenes typing (Anwar et al., 2022 ; Salcedo et al., 2003 ). The approach is based on nucleotide sequences of fragments (loci) of housekeeping genes (usually seven) of approximately of 400–500 bp length of each which can be accurately sequenced on both strands using an automated DNA sequencer (Stessl et al., 2014 ).…”

Section: Molecular Typingmentioning

confidence: 99%

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Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics

Osek

Lachtara

Wieczorek

2022

Food Science & Nutrition

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Listeria monocytogenes is an important foodborne pathogen, which is able to persist in the food production environments. The presence of these bacteria in different niches makes them a potential threat for public health. In the present review, the current information on the classical and alternative methods used for isolation and identification of L. monocytogenes in food have been described. Although these techniques are usually simple, standardized, inexpensive, and are routinely used in many food testing laboratories, several alternative molecular‐based approaches for the bacteria detection in food and food production environments have been developed. They are characterized by the high sample throughput, a short time of analysis, and cost‐effectiveness. However, these methods are important for the routine testing toward the presence and number of L. monocytogenes, but are not suitable for characteristics and typing of the bacterial isolates, which are crucial in the study of listeriosis infections. For these purposes, novel approaches, with a high discriminatory power to genetically distinguish the strains during epidemiological studies, have been developed, e.g., whole‐genome sequence‐based techniques such as NGS which provide an opportunity to perform comparison between strains of the same species. In the present review, we have shown a short description of the principles of microbiological, alternative, and modern methods of detection of L. monocytogenes in foods and characterization of the isolates for epidemiological purposes. According to our knowledge, similar comprehensive papers on such subject have not been recently published, and we hope that the current review may be interesting for research communities.

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Risk factor-based clustering of Listeria monocytogenes in food processing environments using principal component analysis

Nogueira,

Cabo,

García-Sanmartín

et al. 2023

Food Research International

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Genetic diversity, virulence factors, and antimicrobial resistance of Listeria monocytogenes from food, livestock, and clinical samples between 2002 and 2019 in China

Cited by 26 publications

References 73 publications

Prevalence and antimicrobial-resistant characterization of Bacillus cereus isolated from ready-to-eat rice products in Eastern China

Prevalence and antimicrobial-resistant characterization of Bacillus cereus isolated from ready-to-eat rice products in Eastern China

Listeria monocytogenes in foods—From culture identification to whole‐genome characteristics

Risk factor-based clustering of Listeria monocytogenes in food processing environments using principal component analysis

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