Genetic Diversity Study of Egyptian Barley Cultivars using Sequence-Related Amplified Polymorphism (SRAP) Analysis for Water Stress Tolerance

Abstract

Introduction Drought stress is one of the most important aggressive factors for plant production in the arid and semi-arid regions of the world, which affects morphological, physiological, biochemical and molecular processes in plants. (Zhao et al., 2008). Amongst the crops barley (Hordeum vulgare L.) is the first important winter cereal crop grown in arid and semi-arid regions in North Africa, because of its earliness, ability to escape terminal drought-stress, and grown in areas where irrigation is poorly available. (Ceccarelli et al., 1987). Barley is also considered as a good model species for cereals due to its commonly accessible genetic information (Hayes et al., 2000). Robinson et al. (2000) reported that the development of abiotic stress tolerance in barley depends mainly on using the available genetic variation in both cultivated barley (H. vulgare L.) and wild barley (H. vulgare subsp. spontaneum).

Phenotypic and genotypic diversity analysis of some Egyptian barley cultivars (Hordeum vulgare L.) under different heat stress conditions

“…High polymorphic rate and PIC value suggested a high level of heterogeneity among studied barley cultivars. These results agree with those obtained (Said et al, 2015, Mariey et al, 2018, 2021. Since SRAP marker to evaluate the genetic variations among barley and they suggested that SRAP technology is useful for genetic diversity and relationship analyses, marker assisted selection and genetic map construction in barley.…”

“…Moreover, SRAP marker is an influential technique in assessment of genetic variability, showing high degree of reproducibility, discriminatory power, high polymorphism, plentiful information, larger reproducible, simple, established and more helpful than the other DNA markers (Yang et al, 2010). SRAP markers were successfully used in determining the genetic diversity and relationships among in crops such as barley for abiotic stress such as salinity and drought (Ahmed et al, 2021;Mariey, 2018;2021. For heat stress, SRAP also have been successfully used to measuring the genetic diversity and relationships in many cereal crops such as wheat (Said et al, 2015) and maize (Anwer et al, 2021).…”

Genetic Diversity of Egyptian Barley Cultivars for Water Stress using SSR Markers

“…In this regard, water stress during the grain-filling period reduced grain yield by decreasing the number of tillers m -2 and 1000 grain weight, as it is more critical phase and results in substantial yield losses. Similar observations have been reported in many studies of the water stress response of barley (El Rabey et al, 2012;Mariey et al, 2017;Mariey et al, 2018;Khatab et al, 2019;and Hellal et al, 2019),Which they reported that Water stress treatments imposed at different growth stages reduced significantly the grain yield and yield components.…”

“…Genetic diversity and genetic relationship among barley genotypes for drought (water stress) conditions were investigated using different DNA markers such as RAPD (Nazari and Pakniyat, 2010), ISSR markers (Khatab et al, 2019), SCAR marker (Ben Naceur et al, 2018) and SRAP markers (Mariey et al, 2018 andHellal et al, 2019). In this experiment, we using SSR marker to study the genetic diversity among 15 Egyptian barley cultivars for water stress tolerance.Twenty alleles were produced as a result of fingerprinting ten SSR primers ranging from one to four alleles per locus with an average value of 2.0 alleles per locus and the average of PIC value was 0.227 that signified these set of SSR markers were favorably informative.…”

Genetic Diversity among some Egyptian Barley (Hordeum vulgare L.) Cultivars Based on Protein and RAPD Markers

“…This crop is grown in arid and semi-arid regions in the Middle East and North Africa because this crop needs low cultivation inputs such as fertilizer, irrigation and insecticides. Also, tolerance to abiotic stress and containing glutenin protein is important in the industry (Hayes et al, 2000;Kumar et al, 2014 andMariey et al, 2018). In addition to, diploid barley is appropriate example crop to study genome diversity and phenotypic differences that happen during transition from growing landraces to cultivation of new cultivars (Brantestam et al 2007).…”