Genetic diversity, reproductive biology, and speciation in the entomopathogenic fungus<i>Beauveria bassiana</i>(Balsamo) Vuillemin

Devi, K. Uma; Reineke, Annette; Neelapu, Nageswara Rao Reddy; Rao, C. Uma Maheswara; Padmavathi, J.

doi:10.1139/g06-004

Cited by 32 publications

(15 citation statements)

References 34 publications

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“…This last observation is a possible explanation for the lack of grouping between the two Verticillium lecanii strains and the two B. brongmiartii strains present in our collection. Finally, it is important to observe that the lack of correspondence among some of the results from several studies regarding phylogenetics and correlations genotypegeographic origin, genotype-host preference and genotypevirulence is probably due to the using of different DNAanalysis techniques, which may screen different genomic regions, generating diverse outcomes [31].…”

Section: Discussionmentioning

confidence: 99%

Diversidad genética de una colección de hongos entomopatógenos de Ecuador utilizando un enfoque AFLP modificado

Arahana

Bastidas

Torres

et al. 2013

Av. Cienc. Ing. (Quito)

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The genetic diversity of 41 Ecuadorian entomopathogenic fungal strains plus one isolate from the USA, from a collection maintained by INIAP (Instituto Nacional Autónomo de Investigaciones Agropecuarias) was determined using a modified AFLP (Amplified Fragment Length Polymorphism) approach. We found genetic similarity indexes lower than 0.50 and 121 polymorphic bands. The AMOVA analysis revealed that between-group and within-group variation contributed in similar amounts (59% and 41%, respectively) to the whole genetic variation detected. The dendrogram built using Jaccard's genetic coefficient shows twelve groups, where seven of them contain isolates clustered by genus. From these seven groups, five of them contain isolates clustered by the host from which they were recovered. The Bootstrap values show twelve reliable phylogenetic relations with values higher than 70% of confidence. The Principal Components Analysis (PCA) produced six clusters; four of them contain isolates associated by genus. The results suggest the existence of a considerable genetic diversity within the INIAP's entopathogenic fungi collection, and a clustering tendency related to the host from which they were isolated. We did not find common genomic regions among the most virulent entomopathogenic fungi strains. The high genetic diversity found within this collection represents a potential source of genotypes with potent bioinsecticide activity.Keywords. Biocontrol, Genetic Diversity, AFLP, Entomopathogenic fungi, Beauveria, Metarhizium, Verticillium ResumenSe determinó la diversidad genética de 41 cepas ecuatorianas de hongos entomopatógenos y un aislamiento de los EEUU, de una colección mantenida por el INIAP (Instituto Nacional Autónomo de Investigaciones Agropecuarias) utilizando un protocolo modificado de AFLP (Amplified Fragment Length Polymorphism). Se encontraron índices de similitud genéticos inferiores a 0.50 y 121 bandas polimórficas. El análisis AMOVA reveló que la variación entre grupos y dentro de grupos contribuían en cantidades similares (59% y 41% respectivamente) a la variación genética total detectada. El dendograma construido a partir del coeficiente genético de Jaccard muestra doce grupos, de los cuales siete contienen aislamientos agrupados por género. De estos siete grupos, cinco contienen aislamientos agrupados en base al huésped de donde se recolectaron. Los valores de Bootstrap muestran doce relaciones filogenéticas confiables con valores de confianza mayores al 70%. El análisis de componentes principales (PCA) produjo seis grupos; cuatro de ellos contienen aislamientos asociados por género. Estos resultados sugieren la existencia de una diversidad genética considerable dentro de la colección de hongos entomopatógenos del INIAP, y una tendencia de agrupamiento relacionada con el huésped de donde fueron aislados. No se encontraron regiones genómicas comunes dentro de las cepas más virulentas de hongos entomopatógenos. La elevada diversidad genética dentro de esta colección representa una fuente potencial de g...

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Section: Discussionmentioning

confidence: 99%

Diversidad genética de una colección de hongos entomopatógenos de Ecuador utilizando un enfoque AFLP modificado

Arahana

Bastidas

Torres

et al. 2013

Av. Cienc. Ing. (Quito)

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“…has been widely researched to develop biological control agents for pest management, and some of them have had a great impact on the biopesticide market [1,2]. B. bassiana has a relatively broad spectrum against lepidopteran, coleopteran, dipteran, and thysanopteran insect pests [3‐5]. This invertebrate pathogen has high potential in pest management and can be used as an alternative control strategy to overcome the current pest resistance against chemicals and chemical‐mediated environmental residual issues [6,7].…”

Section: Introductionmentioning

confidence: 99%

Gene‐disruption of the entomopathogenic fungus Beauveria bassiana incubated with dsRNA

Shin

Lee

et al. 2021

J Basic Microbiol

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The species of Beauveria bassiana is widely used for the management of agricultural insect pests. In this study, we integrated egfp‐double‐stranded RNA (dsRNA) to a previously generated egfp‐expressing B. bassiana transformant (Bb‐egfp#3) using a protoplast integration method. The Bb‐egfp#3 protoplast was mixed with the dsRNA under PEG/CaCl2 conditions and liquid‐cultured in Sabouraud dextrose broth for 5 days. A control culture followed the same procedure without dsRNA. Bb‐egfp#3/egfp‐dsRNA cultures showed very low fungal growth (OD630 = 0.2) compared to the control culture, Bb‐egfp#3 only (OD630 = 1.1). Screening of possible transformants on Sabouraud dextrose agar revealed a transformant T3, without egfp signal. T3 was confirmed as B. bassiana through sequencing of conserved genes and insect bioassays. Interestingly, the genomic egfp fragment of T3 was disrupted, and the egfp signal was not detected over four subcultures, which was also confirmed by RNA‐seq of Bb‐egfp#3 and T3. This study provides an interesting observation that protoplast integration with dsRNA could possibly generate significantly reduced gene expression in B. bassiana and it is stable across several generations.

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“…Its characterization is based on isozymatic activities, entomopathogenicity, physiological characteristics, and different DNA markers obtained by restriction fragment length polymorphism (RFLP), random amplified polymorphic DNA (RAPD), internal- transcribed-spacer ribosomal region (ITS) nucleotide sequences (4, 20), and microsatellites (7). Considerable genetic variability among B. bassiana isolates obtained from different insects and several geographical origins has been described (6, 10). …”

Section: Introductionmentioning

confidence: 99%

Beauveria bassiana: quercetinase production and genetic diversity

Costa

Pimenta

Luz

et al. 2011

Braz. J. Microbiol.

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Beauveria bassiana genetic diversity and ability to synthesize quercetin 2,3-dioxygenase (quercetinase) were analyzed. B. bassiana isolates, obtained from Brazilian soil samples, produced quercetinase after induction using 0.5 g/L quercetin. B. bassiana ATCC 7159 (29.6 nmol/mL/min) and isolate IP 11 (27.5 nmol/ml/min) showed the best performances and IP 3a (9.5 nmol/mL/min) presented the lowest level of quercetinase activity in the culture supernatant. A high level of polymorphism was detected by random amplified polymorphic DNA (RAPD) analysis. The use of internal-transcribed-spacer ribosomal region restriction fragment length polymorphism (ITS-RFLP) did not reveal characteristic markers to differentiate isolates. However, the ITS1-5.8S-ITS2 region sequence analysis provided more information on polymorphism among the isolates, allowing them to be clustered by relative similarity into three large groups. Correlation was tested according to the Person's correlation. Data of our studies showed, that lower associations among groups, level of quercetinase production, or geographical origin could be observed. This study presents the production of a novel biocatalyst by B. bassiana and suggests the possible industrial application of this fungal species in large-scale biotechnological manufacture of quercetinase.

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Genetic diversity, reproductive biology, and speciation in the entomopathogenic fungusBeauveria bassiana(Balsamo) Vuillemin

Cited by 32 publications

References 34 publications

Diversidad genética de una colección de hongos entomopatógenos de Ecuador utilizando un enfoque AFLP modificado

Diversidad genética de una colección de hongos entomopatógenos de Ecuador utilizando un enfoque AFLP modificado

Gene‐disruption of the entomopathogenic fungus Beauveria bassiana incubated with dsRNA

Beauveria bassiana: quercetinase production and genetic diversity

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