Genetic diversity patterns of Haemonchus contortus isolated from sheep and goats in Bangladesh

Dey, Anita Rani; Zhang, Zhongze; Begum, Nurjahan; Alim, Md. Abdul; Hu, Min; Alam, Mohammad Zahangir

doi:10.1016/j.meegid.2018.12.021

Cited by 28 publications

(25 citation statements)

References 42 publications

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“…Haemonchus spp. are blood sucking nematodes that cause a serious health problem for small ruminant production worldwide, particularly H. contortus that has been recovered from many parts of the world (Achi et al, 2003;Kumsa et al, 2008;Gharamah et al, 2012;Akkari et al, 2013;Yin et al, 2013;Shen et al, 2017;Kandil et al, 2017;Dey et al, 2019). Similarly, goats in Thailand and Lao PDR are now being raised in greater numbers, especially in smallholder form.…”

Section: Discussionmentioning

confidence: 99%

Morphological identification of adult male Haemonchus species in goats from Thailand and Lao PDR

2020

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Section: Discussionmentioning

confidence: 99%

Morphological identification of adult male Haemonchus species in goats from Thailand and Lao PDR

2020

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“…H. contortus inhabits abomasum and a highly fecund parasite. A single parasite of this nematode may cause 0.5 mL blood loss per day leading to acute anemia, edema, and even death in severe affected young animals [27]. Currently, the control of H. contortus infection is anthelmintic based, but frequent use of these products produce drug resistance in strongylid parasites.…”

Section: Introductionmentioning

confidence: 99%

Galectin Domain Containing Protein from Haemonchus contortus Modulates the Immune Functions of Goat PBMCs and Regulates CD4+ T-Helper Cells In Vitro

et al. 2020

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Galectins are glycan-binding proteins that are widely expressed and distributed in mammalian tissues as well as cells of innate and adaptive immune responses. CD4+ T-helper cells differentiate into effector subsets in response to cytokines. T helper 9 cells are one of the recently described subsets of effector T cells that are relatively new and less studied. In this study, galectin domain containing protein from Haemonchus contortus (Hc-GDC) was cloned, expressed in pET32a, and immunoblotting was performed. Localization of recombinant (r)Hc-GDC on outer and inner surface of H. contortus worm and binding with goat Peripheral Blood Mononuclear cells (PBMCs) were performed using immunofluorescence assay. Moreover, effects of rHc-GDC on proliferation, apoptosis, cell migration, and the nitric oxide production in goat PBMCs were evaluated. Furthermore, modulatory effects of rHc-GDC on production of Th1, Th2, and Th9 cells were evaluated by flowcytometry and on interferon gamma, interleukin (IL)-4 and IL-9 were evaluated by quantitative real-time polymerase chain reaction. The results demonstrated that rHc-GDC was successfully cloned, expressed in expression vector as well as in the gut surface of adult H. contortus worm and successful binding with PBMCs surface were observed. Immunoblotting results revealed that rHc-GDC is an important active protein of H. contortus excretory and secretory products. Moreover, the interaction of rHc-GDC with host cells increased the production of Th2, Th9 cells, IL4, IL-9, PBMC proliferation, nitric oxide, and cell migration. No effects of rHc-GDC were observed on PMBC apoptosis, production of Th1 cells, and secretions of IFN-γ and IL-10 cytokines. These findings indicate that recombinant GDC protein from H. contortus modulates the immune functions of goat PBMCs and has the potential to enhance protective immunity by inducing T helper-9-derived IL-9 in vitro.

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“…Moreover, the effects of rHc-MMP-12 on cell proliferation were measured by the cell counting kit (CCK-8). Results highlighted that proliferation was significantly increased (ANOVA: F (4,25) = 95.98, P < 0.001) by the PBMCs incubated with 10 μg/ml, 20 μg/ml and 40 μg/ ml concentration of rHc-MMP-12 as compared to the control group. No significant difference was observed between the control and purified pET32a protein groups (Fig.…”

Section: Effects Of Rhc-mmp-12 On Different Functions Of Goat Pbmcsmentioning

confidence: 97%

“…abomasum and has a complex life-cycle with a parasitic and a free-living phase [4]. Hosts become infected after the ingestion of third-stage infective larvae (L3).…”

mentioning

confidence: 99%

Haemonchus contortus: siRNA mediated knockdown of matrix metalloproteinase 12A (MMP-12) results in reduction of infectivity

Naqvi

Gao

et al. 2020

Parasites Vectors

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Background: RNA interference (RNAi) is an important tool to determine the role of genes. RNAi has been widely used to downregulate target molecules, resulting in the reduction of mRNA for protein expression. Matrix metalloprotease 12A (MMP-12) is known to have important roles during embryonic development, organ morphogenesis and pathological processes in animals. However, MMP-12 from Haemonchus contortus has not been characterized.Methods: Haemonchus contortus MMP-12 gene was cloned and recombinant protein of MMP-12 (rHc-MMP-12) was expressed. Binding activities of rHc-MMP-12 to goat peripheral blood mononuclear cells (PBMCs) were assessed by immunofluorescence assay (IFA) and the immuno-regulatory effects of rHc-MMP-12 on cell proliferation and nitric oxide production were observed by co-incubation of rHc-MMP-12 with goat PBMCs. Furthermore, a soaking method was used to knockdown the expression of Hc-MMP12 gene using three siRNA, targeting different regions of the gene and infectivity of effective siRNA on the development of H. contortus was evaluated in goat.Results: rHc-MMP-12 was successfully expressed in an expression vector as well as the tissues of the cuticle of adult H. contortus worms and a successful binding with PBMCs surface were observed. Increased cellular proliferation and nitric oxide production by goat PBMCs was observed in a dose-dependent manner. Quantitative real time PCR (qRT-PCR) results confirmed the successful silencing of Hc-MMP-12 gene in siRNA of 1, 2 and 3 treated third-stage larvae (L3) of H. contortus in vitro. The most efficient qRT-PCR-identified siRNA template was siRNA-2, with a 69% suppression rate compared to the control groups. Moreover, in an in vivo study, silencing of the Hc-MMP-12 gene by siRNA-2 reduced the number of eggs (54.02%), hatchability (16.84%) and worm burden (51.47%) as compared to snRNAtreated control group. In addition, a shorter length of worms in siRNA-2-treated group was observed as compared to control groups. Conclusions:Our results indicate that siRNA-mediated silencing of Hc-MMP-12 gene in H. contortus significantly reduce the egg counts, larval hatchability, and adult worm counts and sizes. The findings of the present study demonstrate important roles of Hc-MMP-12 in the development of H. contortus.

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Genetic diversity patterns of Haemonchus contortus isolated from sheep and goats in Bangladesh

Cited by 28 publications

References 42 publications

Morphological identification of adult male Haemonchus species in goats from Thailand and Lao PDR

Morphological identification of adult male Haemonchus species in goats from Thailand and Lao PDR

Galectin Domain Containing Protein from Haemonchus contortus Modulates the Immune Functions of Goat PBMCs and Regulates CD4+ T-Helper Cells In Vitro

Haemonchus contortus: siRNA mediated knockdown of matrix metalloproteinase 12A (MMP-12) results in reduction of infectivity

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