Genetic diversity of tick-borne pathogenes in Tomsk and environs

Чаусов, Е. В.; Va, Ternovoĭ; Протопопова, Е. В.; Konovalova, Svetlana N.; Коnonova, Yu. V.; Pershikova, Natalia L.; Moskvitina, N. S.; Романенко, В. Н.; Ivanova, N. V.; Bolshakova, N. P.; Москвитин, С. С.; Korobitsin, I. G.; Gashkov, Sergey I.; Tiutenkov, O. Yu.; Kuranova, Valentina N.; Kravchenko, L. B.; Suchkova, N. G.; Agulova, L. P.; Loktev, V. B.

doi:10.1134/s0013873810020090

Cited by 8 publications

(6 citation statements)

References 23 publications

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“…Molecular distances with standard error estimate were conducted using the Kimura 2-parameter model among Ornithodoros 16S sequences and among Borrelia concatened IGS-FLA sequences as used in other phylogenetical pathogens/vectors studies [ 54 , 55 ]. In the final dataset, the analysis involved 165 different sequences grouping 821 16s RNA tick sequences and 105 different sequences grouping 216 IGS-FLA Borrelia sequences.…”

Section: Methodsmentioning

confidence: 99%

The Epidemiology and Geographic Distribution of Relapsing Fever Borreliosis in West and North Africa, with a Review of the Ornithodoros erraticus Complex (Acari: Ixodida)

et al. 2013

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BackgroundRelapsing fever is the most frequent bacterial disease in Africa. Four main vector / pathogen complexes are classically recognized, with the louse Pediculus humanus acting as vector for B. recurrentis and the soft ticks Ornithodoros sonrai, O. erraticus and O. moubata acting as vectors for Borrelia crocidurae, B. hispanica and B. duttonii, respectively. Our aim was to investigate the epidemiology of the disease in West, North and Central Africa.Methods And FindingsFrom 2002 to 2012, we conducted field surveys in 17 African countries and in Spain. We investigated the occurrence of Ornithodoros ticks in rodent burrows in 282 study sites. We collected 1,629 small mammals that may act as reservoir for Borrelia infections. Using molecular methods we studied genetic diversity among Ornithodoros ticks and Borrelia infections in ticks and small mammals. Of 9,870 burrows investigated, 1,196 (12.1%) were inhabited by Ornithodoros ticks. In West Africa, the southern and eastern limits of the vectors and Borrelia infections in ticks and small mammals were 13°N and 01°E, respectively. Molecular studies revealed the occurrence of nine different Ornithodoros species, including five species new for science, with six of them harboring Borrelia infections. Only B. crocidurae was found in West Africa and three Borrelia species were identified in North Africa: B. crocidurae, B. hispanica, and B. merionesi.Conclusions Borrelia Spirochetes responsible for relapsing fever in humans are highly prevalent both in Ornithodoros ticks and small mammals in North and West Africa but Ornithodoros ticks seem absent south of 13°N and small mammals are not infected in these regions. The number of Ornithodoros species acting as vector of relapsing fever is much higher than previously known.

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Section: Methodsmentioning

confidence: 99%

The Epidemiology and Geographic Distribution of Relapsing Fever Borreliosis in West and North Africa, with a Review of the Ornithodoros erraticus Complex (Acari: Ixodida)

et al. 2013

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“…S1 and S2a). The 5 0 and 3 0 untranslated regions were not included in the analysis because of the low informativity of these genome fragments for phylogenetic reconstructions [15][16][17].…”

Section: Phylogenetic Analysismentioning

confidence: 99%

Tick-borne encephalitis virus: reference strain Sofjin and problem of its authenticity

et al. 2011

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The Sofjin strain is one of the first isolates of tick-borne encephalitis virus and, due to its wide distribution in virus collections, it has become the reference strain. Until now, GenBank has recorded several sequences associated with the Sofjin strain that have significant differences between each other. We have sequenced the complete genome of the Sofjin strain from a virus collection and a genome fragment of the two vaccine Sofjin strains. According to phylogenetic analysis, we concluded that the GenBank sequences belong to three independent groups of Sofjin strains of the Far Eastern subtype. Their genetic differences are not a result of microevolution associated with numerous passages. Retrospective analysis of the peculiarities of origin and distribution for each of these groups showed the authenticity of one of them. For the first time, we have determined a complete genome sequence of the authentic reference TBEV strain Sofjin. Two other groups of strains named Sofjin were probably the result of cross-contamination or laboratory error. The high probability of contamination requires the introduction of a new standard for virological laboratories, the key point of which is the obligatory genetic identification of all collection strains.

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“…RNA extraction, reverse transcription, and detection of TBEV and WNV cDNA using RT-PCR, as well as the detection of other bacterial and protozoan pathogens using PCR, were performed as described earlier (Chausov et al 2010). Briefly, a combined RNA/DNA mixture was extracted from 100 μl of the homogenate of ticks and organs of birds using a RIBOSorb (AmpliSens, Moscow, Russia) set and a DNA/RNA Extraction Kit (NPF Litekh, Moscow, Russia), according to the manufacturers' instructions.…”

Section: Pcr Analysismentioning

confidence: 99%

“…This creates preconditions for the dispersion of pathogens from different areas with various epidemiological settings. Numerous pathogens have been found in ticks and vertebrate hosts in the Tomsk region (Chausov et al 2010). In addition to TBEV and Borrelia spp., some other pathogens such as species of Rickettsia, Anaplasma, Babesia, Ehrlichia, and Bartonella were also found.…”

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confidence: 99%

Detection of tick-borne pathogens in wild birds and their ticks in Western Siberia and high level of their mismatch

et al. 2021

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Genetic diversity of tick-borne pathogenes in Tomsk and environs

Cited by 8 publications

References 23 publications

The Epidemiology and Geographic Distribution of Relapsing Fever Borreliosis in West and North Africa, with a Review of the Ornithodoros erraticus Complex (Acari: Ixodida)

The Epidemiology and Geographic Distribution of Relapsing Fever Borreliosis in West and North Africa, with a Review of the Ornithodoros erraticus Complex (Acari: Ixodida)

Tick-borne encephalitis virus: reference strain Sofjin and problem of its authenticity

Detection of tick-borne pathogens in wild birds and their ticks in Western Siberia and high level of their mismatch

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