Genetic diversity of Theileria orientalis in tick vectors detected in Hokkaido and Okinawa, Japan

Yokoyama, Naoki; Sivakumar, Thillaiampalam; Ota, Naomi; Igarashi, Ikuo; Nakamura, Yukio; Yamashina, Hidenari; Matsui, Shirou; Fukumoto, Natsuko; Hata, Hiroshi; Kondo, Seiji; Oshiro, Mamoru; Zakimi, Satoshi; Kuroda, Yasuhiro; Kojima, Naoya; Matsumoto, Kotaro; Inokuma, Hisashi

doi:10.1016/j.meegid.2012.07.007

Cited by 48 publications

(34 citation statements)

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“…The prevalence of B. ovata in Japan was lower than previously reported (24.5% at ShinHidaka farm, Japan) [14]. This observed difference might be due to distribution of the possible vectors in surveyed areas, as our previous study suggested the Haemaphysalis megaspinosa, which was not detected in Otofuke, as the major tick species in Shin-Hidaka [16]. Additionally, B. ovata was not detected in Chinese samples in this study, despite the previous report of the parasite in this country [4].…”

confidence: 62%

“…In this study, we examined 2,034 bovine DNA samples originating from various countries across Asia, Africa and South America using the AMA-1 PCR assay. Blood samples were collected from cattle populations of Japan [16], Mongolia [1], China [12], Vietnam [9], Thailand [2], the Philippines, Sri Lanka [13], Ghana, South Africa and Brazil. In each country, samples were collected across several selected locations.…”

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confidence: 99%

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A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries

et al. 2013

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ABSTRACT. Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B. ovata infection in Japan, Mongolia, and Thailand.

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confidence: 62%

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A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries

et al. 2013

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“…To date, the major piroplasm surface protein (mpsp), small subunit (SSU) of nuclear ribosomal RNA and 23-kDa piroplasm membrane protein (p23) genes as well as the first and second internal transcribed spacers of nuclear ribosomal DNA (ITS-1 and ITS-2) have been used to study the genetic composition of the T. orientalis complex (Sako et al, 1999;Gubbels et al, 2000;Aktas et al, 2006;Ota et al, 2009;Kamau et al, 2011b;Yokoyama et al, 2011). The most widely applied marker has been mpsp (Jeong et al, 2010;Altangerel et al, 2011;Islam et al, 2011;Kamau et al, 2011a;Khukhuu et al, 2011;Yokoyama et al, 2011Yokoyama et al, , 2012Cufos et al, 2012;Eamens et al, 2013;Perera et al, 2013Perera et al, , 2014Sivakumar et al, 2013). Employing this marker, at least 11 genotypes of T. orientalis (i.e., chitose or type 1; ikeda or type 2; buffeli or type 3; types 4-8; N-1; N-2 and N-3) have been defined (reviewed by Sivakumar et al, 2014).…”

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confidence: 99%

Assessment of sequence variability in a p23 gene region within and among three genotypes of the Theileria orientalis complex from south-eastern Australia

Perera

Gasser

Jabbar

2015

Ticks and Tick-borne Diseases

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“…Analysis of the most common genotyping locus (p32), encoding the variable major piroplasm surface protein (MPSP), currently identifies 11 distinct T. orientalis genotypes (13,15,16). Of these genotypes, type 2 (Ikeda) and to a lesser extent type 1 (Chitose) are typically found in association with clinical disease (6,9,10,(17)(18)(19)(20)(21)(22). The presence of pathogenic and benign forms of T. orientalis greatly complicates its clinical diagnosis, with standard blood film analysis unable to identify the pathogenic genotypes.…”

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Development and Validation of a Quantitative PCR Assay Using Multiplexed Hydrolysis Probes for Detection and Quantification of Theileria orientalis Isolates and Differentiation of Clinically Relevant Subtypes

et al. 2015

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T heileria orientalis is a vector-borne hemoprotozoan that infects cattle and buffalo and is generally spread by ticks of the Haemaphysalis genus (1-3). Historically, this organism has been referred to as Theileria sergenti, Theileria buffeli, or the T. orientalis/T. sergenti/T. buffeli complex; however, the name T. sergenti is now considered invalid (4), and T. orientalis is commonly used to refer to all (5). From a clinical perspective, T. orientalis can cause anemia, lethargy, jaundice, fever, abortion, and mortality in cattle (6). Clinical infection can also result in decreased milk production in dairy cattle (7). T. orientalis is a conditional pathogen, and while pathogenic forms are largely limited to Eastern Asia and Australasia (5, 6, 8-10), it is frequently detected in asymptomatic animals; these benign forms are globally spread (5,(11)(12)(13)(14). Analysis of the most common genotyping locus (p32), encoding the variable major piroplasm surface protein (MPSP), currently identifies 11 distinct T. orientalis genotypes (13,15,16). Of these genotypes, type 2 (Ikeda) and to a lesser extent type 1 (Chitose) are typically found in association with clinical disease (6,9,10,(17)(18)(19)(20)(21)(22). The presence of pathogenic and benign forms of T. orientalis greatly complicates its clinical diagnosis, with standard blood film analysis unable to identify the pathogenic genotypes.Multiple conventional PCR (cPCR) assays have been published for the identification of T. orientalis in blood samples (9,21,23,24). The most commonly cited assays detect and genotype T. orientalis by amplifying unique regions of the MPSP gene (21). These assays use two universal primers to detect T. orientalis infection and specific forward primers to identify the Ikeda, Chitose, and Buffeli genotypes. While this method is highly sensitive and has been validated in prior studies (19,21), it is not multiplexed and is highly susceptible to PCR inhibitors (6), which are often found in nucleotide extractions obtained from blood (25,26). To overcome inhibition, undiluted and diluted nucleotide extracts can be examined in parallel to prevent false-negative results (6,19,27). However, if multiple reactions per sample are required to determine the presence of infection, the procedure becomes both expensive and time-consuming. Furthermore, cPCR does not give an accurate representation of parasite load and therefore cannot provide an indication of the severity of T. orientalis infection. A recently developed multiplexed tandem PCR (28) is able to discriminate between four genotypes of T. orientalis, but it is only semiquantitative and therefore cannot be used to define a clinical threshold.Hydrolysis probe quantitative PCR (qPCR) assays employ sequence-specific, fluorescently labeled probes attached to duplex-

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Genetic diversity of Theileria orientalis in tick vectors detected in Hokkaido and Okinawa, Japan

Cited by 48 publications

References 24 publications

A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries

A PCR Based Survey of <i>Babesia ovata</i> in Cattle from Various Asian, African and South American Countries

Assessment of sequence variability in a p23 gene region within and among three genotypes of the Theileria orientalis complex from south-eastern Australia

Development and Validation of a Quantitative PCR Assay Using Multiplexed Hydrolysis Probes for Detection and Quantification of Theileria orientalis Isolates and Differentiation of Clinically Relevant Subtypes

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