Genetic diversity of the west European honey bee (Apis mellifera mellifera and A. m. iberica) I. Mitochondrial DNA

Garnery, Lionel; Franck, Pierre; Baudry, Emmanuelle; Vautrin, D.; Cornuet, Jean‐Marie; Solignac, Michel

doi:10.1186/1297-9686-30-s1-s31

Cited by 112 publications

(99 citation statements)

References 15 publications

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“…Thus, western oleasters carrying CE1 could be a feral form (a naturalised form of previously cultivated olives or hybrid forms). A similar situation has also been reported for Western European honeybees (Garnery et al 1998). Consequently, in western Europe, oleasters sampled close to olive groves are most-likely feral populations.…”

Section: Human Influence On the Genetic Differentiation Of The Meditementioning

confidence: 57%

Olea europaea (Oleaceae) phylogeography based on chloroplast DNA polymorphism

et al. 2002

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Chloroplast DNA diversity in the olive ( Olea europaea L.) complex was studied using PCR-RFLP and microsatellite markers. Fifteen chlorotypes were distinguished. We constructed a cpDNA phylogenetic tree in which five clades were recognised and located in distinct geographic areas: clade A in Central and Southern Africa, clade C in Asia, clade M in North-West Africa, clade E1 in the Mediterranean Basin and Sahara, and clade E2 in West Mediterranea. Cultivated olive clustered with Mediterranean and Saharan wild forms (clades E1 and E2). Strong genetic differentiation for cpDNA markers was observed between eastern and western Mediterranean olives, suggesting that these areas have represented different glacial refugia. Humans most likely spread one eastern chlorotype, preponderant in cultivars, across the western Mediterranean Basin. Its presence in O. e. subsp. laperrinei from the Sahara suggests a possible Mediterranean olive origin in an African population, which may have overlapped in the Southern Mediterranean during the Quaternary.

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Section: Human Influence On the Genetic Differentiation Of The Meditementioning

confidence: 57%

Olea europaea (Oleaceae) phylogeography based on chloroplast DNA polymorphism

et al. 2002

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“…About half of these honeybees were collected between 2003 and 2005 in France and Belgium, and the other half was cited in the literature by various authors (Collet et al 2006;De la Rúa et al 2001a;Franck et al 1998Franck et al , 2000aFranck et al , 2001Garnery et al 1993Garnery et al , 1995Garnery et al , 1998aJensen et al 2005a;Miguel et al 2007). Samplings quoted several times by authors (Franck et al 2000a;Garnery et al 1993Garnery et al , 1995Garnery et al , 1998aMiguel et al 2007) were only counted once and studies which sample size was not described (Franck et al 1998(Franck et al , 2001 could not be included in this study.…”

Section: Honeybee Samplesmentioning

confidence: 97%

“…The haplotype M9, which has three Q sequences (Franck et al 2000a(Franck et al , 2001Garnery et al 1998a;Miguel et al 2007) was renamed M9'. The haplotype M13 (Garnery et al 1998a) showed a sequence close to M10 0 and was hence renamed M10a 0 . The haplotype M11 (Garnery et al 1998a) had the same sequence as the haplotype M4 0 .…”

Section: Description Of the M Haplotypesmentioning

confidence: 99%

“…Since the validation of the DraI COI-COII test (Garnery et al 1993), data on the diversity found within the A, M, C and O lineages have accumulated, in particular for the M lineage (Collet et al 2006;De la Rúa et al 2001a, 2005Franck et al 1998Franck et al , 2000aFranck et al , 2001Garnery et al 1993Garnery et al , 1995Garnery et al , 1998aJensen et al 2005a;Miguel et al 2007) which provides a comprehensive database for the conservation of the black honeybee. However, since most of the M haplotypes were estimated directly on gels, by eye, they need to be sequenced and revised before they can be used in conservation planning.…”

Section: Introductionmentioning

confidence: 99%

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Review of the DraI COI-COII test for the conservation of the black honeybee (Apis mellifera mellifera)

Rortais

Arnold

Alburaki

et al. 2010

Conservation Genet Resour

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The black honeybee (Apis mellifera mellifera; M lineage) which is affected by widespread colony losses, colony introgression and population shifts within its natural range of distribution, needs proper protection and management planning. The DraI COI-COII test discriminates between and within honeybee lineages (M, C, A and O). With this test, a global assessment of the diversity found in the M lineage (i.e. description of haplotype frequencies, fragment length and size, nucleotide sequences) is made with colonies newly sampled in France and Belgium (n = 3,261 colonies) and previously described in the literature (n = 3,367). A total of 91 M haplotypes were described including 52 new ones. The haplotypes M4 and M4' were the most frequent followed by M17, M7, M6, M19, M8, M17' and by many rare ones. Finally, these data were used to infer hypotheses on the putative origin of the M haplotypic diversity and to determine a protocol for the establishment of protected areas of black honeybee colonies. This protocol is based on the DraI COI-COII test and on case-studies of protected areas of black honeybees from France.

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“…Morphological discrimination of Greek honey bees Tarès et al, 1993), mitochondrial DNA (mtDNA) (Moritz et al, 1986;Smith et al, 1989;Smith, 1991;Hunt and Page, 1992;Garnery et al, 1993;Oldroyd et al, 1995;Arias and Sheppard, 1996;Pedersen, 1996;De la Rúa et al, 2000), and microsatellites (Estoup et al, 1993;Garnery et al, 1998), are also used to study genetic variability in honey bees. Several attempts have been made to simplify morphological determination, such as reducing the number of characters (Dedej and Nazzi, 1994;Cermak and Kaspar, 2000), using a single wing cell (Francoy et al, 2006), or using computer software designed specifically for morphometric measurements (Daly et al, 1982;Batra, 1988;Tofilski, 2004).…”

mentioning

confidence: 99%

Morphological Discrimination of Greek Honey Bee Populations Based on Geometric Morphometrics Analysis of Wing Shape

Charistos¹,

Hatjina²,

Bouga

et al. 2014

Journal of Apicultural Science

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a b s t r a c t honey bees collected from 32 different localities in greece were studied based on the geometric morphometrics approach using the coordinates of 19 landmarks located at wing vein intersections. procrustes analysis, principal component analysis, and canonical variate analysis (cVa) detected population variability among the studied samples. according to the principal component analysis (pca) of pooled data from each locality, the most differentiated populations were the populations from the aegean island localities astypalaia, chios, and kythira. however, the populations with the most distant according to the canonical variate analysis performed on all measurements were the populations from heraklion and chania (both from crete island). these results can be used as a starting point for the use of geometric morphometrics in the discrimination of honey bee populations in greece and the establishment of conservation areas for local honey bee populations.keywords: apis mellifera, geometric morphometrics, greece, morphological discrimination. Traditionally, intraspecific taxonomy of the honey bee Apis mellifera L. has been based on morphology. Twenty-seven subspecies of A. mellifera are currently recognized on the basis of morphometric characteristics (Ruttner, 1988(Ruttner, , 1992Sheppard et al., 1997;Engel, 1999;Sheppard and Meixner, 2003). These subspecies are also described as 'geographic races' because their distributions correspond to distinct geographic areas. (Garnery et al., 1993; Arias and Sheppard, 1996;Franck et al., 2000Franck et al., , 2001Miguel et al., 2007;Cánovas et al., 2008). Traditional morphometrics, so-called classical morphometry, was the only method to describe the diversity of honey bees for a long time (Ruttner, 1988), but biochemical methods have been developed (i.e., allozymes, isoenzymes) (Nunamaker and Wilson, 1982; Badino et al., 1988;Bouga et al., 2005b;Ivanova et al., 2012) 76Morphological discrimination of Greek honey bees Tarès et al., 1993), mitochondrial DNA (mtDNA) (Moritz et al., 1986;Smith et al., 1989;Smith, 1991;Hunt and Page, 1992;Garnery et al., 1993;Oldroyd et al., 1995; Arias and Sheppard, 1996;Pedersen, 1996;De la Rúa et al., 2000), and microsatellites (Estoup et al., 1993;Garnery et al., 1998), are also used to study genetic variability in honey bees. Several attempts have been made to simplify morphological determination, such as reducing the number of characters (Dedej and Nazzi, 1994;Cermak and Kaspar, 2000), using a single wing cell (Francoy et al., 2006), or using computer software designed specifically for morphometric measurements (Daly et al., 1982; Batra, 1988;Tofilski, 2004). Dupraw (1964Dupraw ( , 1965a was the first to use a set of quantitative characteristics for wing venation and correctly classified many subspecies. In 1978, Daly and Balling successfully applied this method to differentiate Africanized and European honey bees in South America. Later, Daly et al. (1982) began to use digital measurements to investigate the morphometrics of ...

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Genetic diversity of the west European honey bee (Apis mellifera mellifera and A. m. iberica) I. Mitochondrial DNA

Abstract: -Variability of mitochondrial deoxyribonucleic acid (mtDNA)

Cited by 112 publications

References 15 publications

Olea europaea (Oleaceae) phylogeography based on chloroplast DNA polymorphism

Olea europaea (Oleaceae) phylogeography based on chloroplast DNA polymorphism

Review of the DraI COI-COII test for the conservation of the black honeybee (Apis mellifera mellifera)

Morphological Discrimination of Greek Honey Bee Populations Based on Geometric Morphometrics Analysis of Wing Shape

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