Genetic diversity of the Salix lapponum L. population intended as a source of material for reintroduction

Głębocka, Katarzyna; Pogorzelec, Magdalena

doi:10.12657/denbio.078.013

Cited by 9 publications

(7 citation statements)

References 31 publications

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“…For example, in Europe, restoration programs involving habitats with rare species of the genus Salix are carried out in the United Kingdom [48], the Czech Republic [49], and Poland [50], among others. These programs are usually linked to the verification of methods that increase the effectiveness of conservation treatments and to multifaceted studies of factors affecting the stability of populations of endangered species [15][16][17]20,34,[51][52][53][54][55][56][57][58][59].…”

Section: Of 15mentioning

confidence: 99%

Microhabitat Conditions and Inter-Species Competition Predict the Successful Restoration of Declining Relict Species Populations

Kamocki

Kołos

Pogorzelec

et al. 2022

IJERPH

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The local populations of relict plant taxa living near the limits of their geographical range are particularly vulnerable to extinction. For example, Salix lapponum is one of the rarest and most endangered glacial relicts in Western and Central Europe. In Poland, the number of its sites has dramatically decreased over the past few decades, prompting us to take conservation measures focused on saving endangered populations. During a field experiment aimed at the reconstruction of the downy willow population in the Knyszyn Forest (NE Poland), 730 individuals of the species were planted in four different natural sites. The seedlings were obtained by micropropagation from parts of vegetative individuals taken from the most abundant population of this species in eastern Poland (Lake Bikcze). The success of the reintroduction, measured by the number of individuals that survived 2–3 years in the wild and took up growth, was about 67%, however, with low flowering efficiency (7.5%). Additionally, monitoring showed significant differences in plant survival and growth rates under different habitat conditions prevailing at the site and with different cover from competing species, especially tall grasses. However, the restoration projects for relict shrub species should include periodic removal of competing plants and protection of plants from trampling and browsing by herbivorous mammals to increase reintroduction success.

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Section: Of 15mentioning

confidence: 99%

Microhabitat Conditions and Inter-Species Competition Predict the Successful Restoration of Declining Relict Species Populations

Kamocki

Kołos

Pogorzelec

et al. 2022

IJERPH

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“…Pieces of S. lapponum shoots were collected from the largest population in eastern Poland, located in a peat bog by Lake Bikcze (51 • 22 724" N 023 • 02 563" E), at the end of April 2018. The population has a satisfactory sex ratio and high genetic variation [9], which indicates its ability to reproduce sexually. Shoot fragments 5 cm in length were collected from mother plants, which had been previously numbered and whose sex had been verified.…”

Section: Tissue Culture Initiation and Stabilizationmentioning

confidence: 99%

“…The results of genetic studies have also confirmed the absence of clones in populations of this species. However, there were no seedlings observed within the existing populations, most likely due to the unstable habitat conditions, in which seeds were unable to germinate [9].…”

Section: Introductionmentioning

confidence: 99%

Genetic Stability of the Endangered Species Salix lapponum L. Regenerated In Vitro during the Reintroduction Process

Parzymies

Pogorzelec

Głębocka

et al. 2020

Biology

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Salix lapponum L. is a boreal relict species, threatened with extinction in Poland. An 80% decrease in the number of its stands was confirmed in the last half-century, so that to prevent the loss of downy willow, attempts were made to reintroduce this species in natural habitats. Micropropagation was chosen as a first stage of its active conservation. S. lapponum shoots were collected and disinfected with NaOCl, AgNO3, or HgCl2 or with a two-step disinfection with NaOCl and then placed on MS medium with BA 1 mg·dm−3 and IBA 0.1 mg·dm−3. Regenerated shoots were cultivated with addition of BA, KIN, or 2iP, alone or in combination with auxins, to find the highest multiplication rate. Inter-simple sequence repeat (ISSR) analysis and flow cytometric analyses were conducted on in vitro regenerated plants to check their genetic stability. Disinfection was quite difficult and the use of HgCl2 was the most efficient. The highest multiplication rate was obtained in presence of KIN at 0.5 mg·dm−3 + IAA at 0.5 mg·dm−3. The analysis confirmed the genome size stability, which is in agreement with the results obtained by ISSR, revealing no somaclonal variation in plantlets and therefore allowing the use of the obtained plants for reintroduction.

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“…This particular population was selected due to its size (the largest in Eastern Poland -about 300 individuals), the stable sex ratio (1:1) and optimal genetic structure (lack of clonal individuals) that confirms its proper functioning (Głębocka & Pogorzelec, 2017).…”

Section: Study Area and Populationmentioning

confidence: 99%

Salix lapponum L. vs. phytophagous insects – an assessment of the risks and the reaction of plants

Kmieć¹,

Pogorzelec²,

Hawrylak-Nowak³

et al. 2018

Dendrobiology

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Abstract:Since the 1950s, a significant decrease in the population numbers of Salix lapponum L. has been observed in Poland, particularly in the Polesie Lubelskie region. One of the most important threats comes from the biotic environment including interactions at the trophic level. An unidentified issue was the occurrence and importance of phytophagous insects inhabiting S. lapponum in terms of real threats to growth and development as well as specific plant reactions to herbivores. The aim of the study was to recognize the occurrence of phytophagous insects inhabiting S. lapponum, i.e. an endangered species, and estimation of physiological changes caused by the herbivorous species feeding. The field study was conducted in a locality of S. lapponum population in the peat bog near Bikcze Lake (Polesie Lubelskie Region) in 2014-2015. In each growing season from the end of April to September, 100 stems of S. lapponum shoots with similar length were examined once a month. Selected parameters of chlorophyll a fluorescence were measured and the accumulation of H 2 O 2 was visually detected in leaves collected from the control plants and from those attacked by herbivores using the 3',3-diaminobenzidine (DAB) method. We found the presence of 8 phytophagous insect species from 4 orders (Coleoptera, Diptera, Hemiptera and Lepidoptera) on S. lapponum. Most of the shoots were inhabited by Lochmea caprea, Aphrophora salicina and Aphis farinosa. On the basis of the measurements of chlorophyll a fluorescence and H 2 O 2 accumulation under phytophagous insects feeding, we have demonstrated that S. lapponum was able to maintain photosynthesis under insect attack and that the accumulation of H 2 O 2 in leaf blades indicates local rather than systemic stress response to herbivore feeding. Phytophagous insects feeding caused relatively weak disruptions to host plant physiology and S. lapponum is able to maintain photosynthetic efficiency under insect attack. Variation in plant traits influence host plant selection and performance of herbivorous insects. Comparative studies are needed to assess the species composition and population numbers of insects occurring on plants from other S. lapponum populations. Detailed knowledge of the biotic factors affecting the occurrence of S. lapponum would facilitate establishment of the form of its conservation.

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Genetic diversity of the Salix lapponum L. population intended as a source of material for reintroduction

Cited by 9 publications

References 31 publications

Microhabitat Conditions and Inter-Species Competition Predict the Successful Restoration of Declining Relict Species Populations

Microhabitat Conditions and Inter-Species Competition Predict the Successful Restoration of Declining Relict Species Populations

Genetic Stability of the Endangered Species Salix lapponum L. Regenerated In Vitro during the Reintroduction Process

Salix lapponum L. vs. phytophagous insects – an assessment of the risks and the reaction of plants

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