Genetic diversity of the golden potato cyst nematode Globodera rostochiensis and determination of the origin of populations in Quebec, Canada

Worldwide invasion routes of the pinewood nematode: What can we infer from population genetics analyses?

“…Deciphering geographic pathways followed by invasive species is a key first step in this direction. Many studies carried out over the last decade have tried to identify the invasion routes of several invasive species, including insects (Papura et al 2012;Pascual et al 2007;Perdereau et al 2013;Zepeda-Paulo et al 2010), plants (Kelager et al 2013), nematodes (Boucher et al 2013) and fungi (Fontaine et al 2013). Indeed, this approach can be used to address various questions of both practical and theoretical concern .…”

Resistance genes against plant-parasitic nematodes: a durable control strategy?

“…Primary founder events, such as the introduction of cyst nematodes into Europe and North America, are generally associated with a reduction in genetic variation in the introduced population in comparison to the source population (Nei et al, 1975;Bryant et al, 1986;Tajima, 1989). Molecular studies have demonstrated reduced allelic richness in European and North American populations of G. rostochiensis in comparison to populations from Bolivia (Boucher et al, 2013). Furthermore, a reduction in allelic richness was demonstrated in European G. pallida populations in comparison to populations from southern Peru, but similar levels of allelic diversity were observed when European populations were compared to populations from northern Peru (Plantard et al, 2008).…”

Human influence on the dispersal and genetic structure of French Globodera tabacum populations

“…In order to assess the genetic variability of TCN populations through microsatellite scoring, DNA from individual second-stage juveniles (J2) was extracted using a protocol based on sodium hydroxide and proteinase K as detailed in Boucher et al (2013). Each individual was taken from separate cysts.…”

“…Two markers (Gp108, Gp116) were developed from G. pallida available genomic sequences (J. Montarry, unpublished data) and seven markers were developed from a G. rostochiensis microsatellite library (see Boucher et al, 2013). Two multiplex panels were defined to reduce the time required to genotype the 553 DNAs at the nine loci (Multiplex Manager Software - Holleley and Geerts, 2009) (Table 2).…”

“…More direct human impacts on the evolution of plant parasitic nematodes have till now been restricted to the role of human movements and commercial trade in the translocation of organisms and their parasites into novel areas. Such issues have been tackled in plant parasitic nematodes like the potato cyst nematodes Globodera pallida and Globodera rostochiensis (Plantard et al, 2008;Boucher et al, 2013) and the pinewood nematode Bursaphelenchus xylophilus (Jung et al, 2010;Valadas et al, 2012;Mallez et al, 2013).…”