GENETIC DIVERSITY OF THE ENDEMIC SPECIES SHABBOUT (Arabibarbus grypus (HECKEL, 1843)) BASED ON PARTIAL CYTOCHROME B SEQUENCES OF MITOCHONDRIAL DNA

Parmaksız, Arif; Şeker, Özlem

doi:10.3153/ar18011

Cited by 8 publications

(4 citation statements)

References 12 publications

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“…In this study, the Polymerase Chain Reaction (PCR) process was carried out in a thermal cycler (BIO-RAD T100 TM ) device. The primer sequence used for the mtDNA cyt b gene region was taken from Briolay et al (1998), and all PCR chemicals and conditions were made according to the criteria in the Parmaksız and Şeker (2018) study. The primer sequence used for amplification of the mtDNA d loop region is Iguchi et al (1997), taken from Inoue et al (2000) studies, and all PCR chemicals and conditions were adapted according to Oymak and Parmaksız (2018) study.…”

Section: Methodsmentioning

confidence: 99%

“…The delicious meat of the A. grypus species causes it to be preferred by the local people and increases its economic importance. Therefore, there is excessive hunting pressure on the populations of this species and it is known that its stocks are decreasing day by day (Parmaksız and Şeker, 2018). This species is sensitive in the "VU" (Vulnerable/Sensitive) category according to the 2020 IUCN criteria (Bayhan, 2021).…”

Section: Introductionmentioning

confidence: 99%

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Mitochondrial DNA sequence analysis of Arabibarbus grypus (Heckel, 1843) living in Great Zab (Erbil, Iraq)

Parmaksız¹

2023

Aquat Res

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Arabibarbus grypus (Heckel, 1843), which naturally lives in the Euphrates and Tigris River systems and is endemic, is an economically important fish species consumed by humans. Since the population of this species, which is hunted by both fishermen and local peo-ple, is decreasing day by day, its genetic characteristics need to be determined. This study aims to determine the genetic characteristics of A. grypus samples living in Great Zab based on sequence analysis of mtDNA cyt b and mtDNA d loop gene regions. Total DNA isolation was performed from muscle tissue using the kit. Then, Polymerase Chain Reac-tion was applied through spe-cific primers to mtDNA cyt b and mtDNA d loop gene re-gions, and the target regions were amplified. The products with the target length were sent to the commercial firm and sequence analysis was per-formed. Regarding these spec-imens living in Great Zab, two haplotypes were determined for the mtDNA cyt b gene re-gion and five haplotypes for the mtDNA d loop gene re-gion. These haplotypes were compared with the haplotypes in the gene bank and the re-sults were evaluated. Some important data has been ob-tained regarding the conserva-tion and management of this fish species. Since three new haplotypes were detected for the d loop region in this stud-ied locality, it is important to include the samples in this lo-cality in conservation studies.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Mitochondrial DNA sequence analysis of Arabibarbus grypus (Heckel, 1843) living in Great Zab (Erbil, Iraq)

Parmaksız¹

2023

Aquat Res

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“…PCR conditions and chemicals were applied according to the study of Parmaksız and Eskici (2018) and the product was obtained [20]. The primer sequence used for the mtDNA cyt b gene region was taken from Briolay et al, (1998) (L15267 F: 5ˈ GTT TGA TCC CGT TTC GTG TA-3ˈ; H15891 R: 5'AAT GAC TTG AAG AAC CAC CGT-3'), PCR conditions and chemicals were applied according to the study of Parmaksız and Şeker (2018) and the product was obtained [21,22].…”

Section: Dna Isolation and Pcrmentioning

confidence: 99%

mtDNA COI and Cyt b Analysis of Carassius auratus (Linnaeus, 1758) Species Living in Atatürk Dam Lake (Turkey)

Parmaksız

Demir

Ulusal

2022

International Journal of Life Sciences and Biotechnology

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Today, biological invasion is one of the leading threats to biodiversity and is an issue that is gaining more and more importance. In recent years, molecular techniques have been widely used in the identification and monitoring of invasive species in many parts of the world. This study aimed to detect the infested species, Carassius auratus, in Atatürk Dam Lake and to reveal the population status. For these purposes, 10 Carassius auratus specimens were randomly selected from Atatürk Dam Lake. After DNA isolation of the selected samples, mtDNA COI and cyt b gene regions were sequenced and analyzed. In this study, sequence analyzes of individuals belonging to the C. auratus species living in Atatürk Dam Lake were performed for the first time using mtDNA COI and cyt b markers. Analyzed sequence results were compared with databases and it was concluded that the results obtained for both mtDNA markers were compatible. No other variation was observed. When the results are evaluated as a whole, it is possible to say that this species has spread recently. In future studies, it is recommended to determine the invasive species populations that are intensely found in Atatürk Dam Lake and other lakes and to determine the necessary strategies to combat these invasive species

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“…ilk denatürasyon, 95°C'de 30 s. denatürasyon, 57°C'de 30 s. bağlanma ve 72°C'de 45 s. uzama olmak üzere toplam 35 döngü gerçekleştirilmiş, son olarak örnekler 72°C'de 10 dak. tutularak sonlandırılmıştır (Parmaksız & Şeker, 2018).…”

Section: Dna İzolasyonu Ve Pcrunclassified

Atatürk Baraj Gölü’nde (Türkiye) Yaşayan Carassius gibelio (Bloch, 1782) türünün mtDNA COI ve cyt b Analizi

Parmaksız¹,

Demir²

2022

tjbc

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Bu çalışmanın amacı, Atatürk Baraj Gölü'nde yaşayan C. gibelio türünün mtDNA COI ve Cyt b gen bölgelerine ait sekanslara dayalı olarak gen bankasındaki sekanslarla karşılaştırılması ve filogenetik analizlerin yapılmasıdır. Materyal ve Yöntem: Atatürk Baraj Gölü Bozova bölgesinde yaşayan hedef türe ait bireyler balıkçılardan rastgele seçilerek satın alınmıştır. Total DNA izolasyonu, GeneJET Genomic DNA Purification Kit (Thermo Scientific) kullanılarak kas dokusundan protokol talimatları doğrultusunda yapılmıştır. Daha sonra mtDNA COI ve Cyt-b bölgelerine ait özgül primerler kullanılarak ilgili bölgeler PCR yöntemi ile çoğaltılmıştır. Elde edilen PCR ürünleri Agaroz jelde yürütülmüş ve ürün oluşturan örnekler seçilerek hizmet alımı şeklinde ticari firmaya gönderilmiş ve 3500 XL Genetic Analyzer cihazı ile dizi analizi yaptırılmıştır. Bulgular: mtDNA COI ve cyt b gen bölgeleri için sırayla 620 ve 580 bp uzunlukta sekanslar elde edilmiştir. mtDNA COI bölgesi için daha önce Türkiye'de yaşayan bireylerle aynı haplotipler tespit edilmesine rağmen mtDNA Cyt b gen bölgesi için daha önce tanımlanmayan yeni bir haplotip tespit edilmiştir. Sonuç: Atatürk Baraj Gölü'nde yaşayan C. gibelio türüne ait bireylerin mtDNA COI ve Cyt b gen bölgelerine ait sekans analizleri ilk defa bu çalışmada yapılmış ve gen bankasındaki verilerle karşılaştırılmıştır. Anahtar Kelimeler: Atatürk Baraj Gölü, Carassius gibelio, mtDNA, COI, cyt b mtDNA COI and cyt b Analysis of Carassius gibelio (Bloch, 1782) Living in Atatürk Dam Lake (Turkey)

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GENETIC DIVERSITY OF THE ENDEMIC SPECIES SHABBOUT (Arabibarbus grypus (HECKEL, 1843)) BASED ON PARTIAL CYTOCHROME B SEQUENCES OF MITOCHONDRIAL DNA

Cited by 8 publications

References 12 publications

Mitochondrial DNA sequence analysis of Arabibarbus grypus (Heckel, 1843) living in Great Zab (Erbil, Iraq)

Mitochondrial DNA sequence analysis of Arabibarbus grypus (Heckel, 1843) living in Great Zab (Erbil, Iraq)

mtDNA COI and Cyt b Analysis of Carassius auratus (Linnaeus, 1758) Species Living in Atatürk Dam Lake (Turkey)

Atatürk Baraj Gölü’nde (Türkiye) Yaşayan Carassius gibelio (Bloch, 1782) türünün mtDNA COI ve cyt b Analizi

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