Genetic diversity of the Chinese liver fluke Clonorchis sinensis from Russia and Vietnam

Челомина, Г. Н.; Tatonova, Yulia V.; Hùng, Nguyễn Mạnh; Ngo, Ha Duy

doi:10.1016/j.ijpara.2014.06.009

Cited by 26 publications

(13 citation statements)

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“…nov. differs from M. bilis, M. xanthosomus , and M. orientalis in most metrics, as well as the length/width body ratio and ventral/oral sucker ratio (Table ). Besides the determination of the phylogenetic status for the Metorchis representative in the Russian southern Far East, we analyzed the levels of nucleotide and haplotype diversities for the cox1 gene, which indicate long‐term isolation of a relatively small population (Avise, ), as was previously revealed for Clonorchis sinensis from the same geographic territory (Chelomina et al., ). Almost all M. ussuriensis sp.…”

Section: Discussionmentioning

confidence: 99%

“…and ( Paragonimus westermani ). The partial cox1 gene sequences were amplified using the forward 5cF22 (5′‐TAG‐ACT‐ATC‐TGT‐CTT‐CAA‐AAC‐A‐3′ (Chelomina, Tatonova, Hung, & Ngo, )) and reverse CO1‐Rv (5′‐AAC‐AAA‐TCA‐TGA‐TGC‐AAA‐AGG‐TA‐3′ (Katokhin et al., )) primers. The partial sequences of 28S were amplified using the following primers: forward dig12 (5′‐AAG‐CAT‐ATC‐ACT‐AAG‐CGG‐3′) and reverse 1500R (5′‐GCT‐ATC‐CTG‐AGG‐GAA‐ACT‐TCG‐3′) (Tkach, Littlewood, Olson, Kinsella, & Swiderski, ).…”

Section: Methodsmentioning

confidence: 99%

“…). Besides the determination of the phylogenetic status for the Metorchis representative in the Russian southern Far East, we analyzed the levels of nucleotide and haplotype diversities for the cox1 gene, which indicate long-term isolation of a relatively small population(Avise, 2000), as was previously revealed for Clonorchis sinensis from the same geographic territory(Chelomina et al, 2014).Almost all M. ussuriensis sp. nov. samples for genetic analysis were obtained from experiments with naturally infected second intermediate hosts, and this explains the number of different haplotypes in one population.In contrast to the markers mentioned above, in the phylogenetic tree based on ITS2 sequences, M. ussuriensis sp.…”

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Life cycle, morphology of developmental stages ofMetorchis ussuriensissp. nov. (Trematoda: Opisthorchiidae), and phylogenetic relationships with other opisthorchiids

Besprozvannykh

Tatonova

Shumenko

2018

J Zool Syst Evol Res

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The complete life cycle and developmental stages of the fluke, Metorchis ussuriensis sp. nov. (Trematoda, Platyhelminthes), are herein described. The results of the present experiments showed that, for flukes from the Primorsky Region in the Russian southern Far East, the first intermediate hosts are the snails Parafossarulus spiridonovi and Boreoelona ussuriensis, and the second intermediate hosts are freshwater fish, tadpoles, and snails. The definitive host in this experiment was Anas platyrhynchos dom. Morphometric parameters of M. ussuriensis sp. nov. demonstrate similarities with Metorchis taiwanensis, but the two species differ in the sizes of their bodies, sizes of suckers of adult worms, and sizes of cercariae, as well as respective positions of the finfold in cercariae. Phylogenetic reconstructions and genetic distances using the cox1 gene sequences support the conclusion that M. ussuriensis sp. nov. is well distinguished from all other species of the genus Metorchis, while sequences of internal transcribed spacers (ITS1 and ITS2) failed to separate M. ussuriensis sp. nov., Metorchis bilis, and Metorchis xanthosomus. In addition, we sequenced 1,402 bp of the 28SrRNA gene of M. ussuriensis sp. nov. being the first 28S sequences in the genus Metorchis. Comparison to other trematodes suggests that 28SrRNA could proof suitable for the differentiation of trematode species.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Life cycle, morphology of developmental stages ofMetorchis ussuriensissp. nov. (Trematoda: Opisthorchiidae), and phylogenetic relationships with other opisthorchiids

Besprozvannykh

Tatonova

Shumenko

2018

J Zool Syst Evol Res

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“…Currently, most published molecular evidence seems to suggest that C. sinensis represents a single species and that genetic variation within the species is relatively low (Park and Yong, 2001;Lee and Huh, 2004;Petney et al, 2013). However, to date, most studies have used a relatively small number of genetic loci for specific identification (e.g., internal transcribed spacers of nuclear ribosomal DNA) and/or population genetic studies (e.g., act, tub, ef-1a, cox1, cox3, nad4 and nad5) (Park and Yong, 2001;Lee and Huh, 2004;Liu et al, 2007;Park, 2007;Katokhin et al, 2008;Lai et al, 2008;Shekhovtsov et al, 2009;Liu et al, 2012;Shin et al, 2013;Sun et al, 2013;Tatonova et al, 2013;Xiao et al, 2013;Chelomina et al, 2014), and it is not yet known whether "cryptic" (= morphologically similar, but genetically distinct) species exist within C. sinensis. Nonetheless, karyotypic differences in C. sinensis observed among China, Korea (2n = 56) and the Russian Far East (2n = 14) (Park and Yong, 2001;Zadesenets et al, 2012) do suggest that this might be the case.…”

Section: Introductionmentioning

confidence: 99%

Mitochondrial genomic comparison of Clonorchis sinensis from South Korea with other isolates of this species

Wang

Young

Koehler

et al. 2017

Infection, Genetics and Evolution

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Clonorchiasis is a neglected tropical disease that affects >35 million people mainly in China, Vietnam, South Korea and some parts of Russia. The disease-causing agent, Clonorchis sinensis, is a liver fluke of humans and other piscivorous animals, and has a complex aquatic life cycle involving snails and fish intermediate hosts. Chronic infection in humans causes liver disease and associated complications including malignant bile duct cancer. Central to control and to understanding the epidemiology of this disease is knowledge of the specific identity of the causative agent as well as genetic variation within and among populations of this parasite. Although most published molecular studies seem to suggest that C. sinensis represents a single species and that genetic variation within the species is limited, karyotypic variation within C. sinensis among China, Korea (2n=56) and Russian Far East (2n=14) suggests that this taxon might contain sibling species. Here, we assessed and applied a deep sequencing-bioinformatic approach to sequence and define a reference mitochondrial (mt) genome for a particular isolate of C. sinensis from Korea (Cs-k2), to confirm its specific identity, and compared this mt genome with homologous data sets available for this species. Comparative analyses revealed consistency in the number and structure of genes as well as in the lengths of protein-coding genes, and limited genetic variation among isolates of C. sinensis. Phylogenetic analyses of amino acid sequences predicted from mt genes showed that representatives of C. sinensis clustered together, with absolute nodal support, to the exclusion of other liver fluke representatives, but sub-structuring within C. sinensis was not well supported. The plan now is to proceed with the sequencing, assembly and annotation of a high quality draft nuclear genome of this defined isolate (Cs-k2) as a basis for a detailed investigation of molecular variation within C. sinensis from disparate geographical locations in parts of Asia and to prospect for cryptic species.

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“…There are ITS1 rDNA data for C. sinensis from different areas (Tatonova et al, 2012;Sun et al, 2013). Vietnam is the southernmost area for this parasite and previously, C. sinensis from Vietnam has been investigated using mitochondrial cox1 gene (Chelomina et al, 2014), while there are no data on the ITS1 marker in this area. Also, O. viverrini from Vietnam has not been analysed using the ITS1 rDNA marker.…”

Section: Introductionmentioning

confidence: 99%

Genetic data of the ITS1-5.8S rDNA sequences of small liver flukes (Opisthorchiidae) from cats in Northern Vietnam

Hùng¹,

Ho²,

Tatonova³

2020

Vietnam J. Biotechnol.

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Domestic cats and humans are favorite final hosts for some hepatic trematodes of Opisthorchiidae. The present study examined the sequence variation in the nuclear ribosomal DNA (rDNA) region, including the internal transcribed spacer (ITS1) and the 5.8S gene, of two human opisthorchiid species Clonorchis sinensis (Cobbold, 1875) Looss, 1907 and Opisthorchis viverrini (Poirier, 1886) Stiles & Hassal, 1896, in cats sampled in Northern Vietnam. The length of 30 partial ITS1‒5.8S rDNA sequences of C. sinensis from different hosts and localities varied from 761 to 766 bp. The results showed completely identity for all investigated 5.8S rDNA sequences of C. sinensis. In contrast, the variations were revealed in the complete ITS1 sequences of C. sinensis. Three polymorphic sites, indel and new sites with intragenomic polymorphism were detected. The length of the complete ITS1 rDNA region ranged from 656 to 661 bp. There are no substitutions downstream of the 5 bp insertion, and the different ratio of nucleotide in polymorphic sites with presence/absence of insertion leads to the existence of 6 genotypes in the investigated samples. The major genotype was obtained in 70% of sequences, other genotypes were presented by 1, 2 or 3 sequences. Nucleotide diversity in whole sample of C. sinensis was 0.00122 ± 0.00073. Partial sequences of ITS1‒5.8S rDNA of O. viverrini were 745 bp in length, and no difference was observed. Phylogenetic analyses by neighbor joining phylogram inferred from ITS1‒5.8S rDNA of 3 sequences of O. viverrini and 30 sequences of C. sinensis were formed two separately monophyletic clades.

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Genetic diversity of the Chinese liver fluke Clonorchis sinensis from Russia and Vietnam

Cited by 26 publications

References 107 publications

Life cycle, morphology of developmental stages ofMetorchis ussuriensissp. nov. (Trematoda: Opisthorchiidae), and phylogenetic relationships with other opisthorchiids

Life cycle, morphology of developmental stages ofMetorchis ussuriensissp. nov. (Trematoda: Opisthorchiidae), and phylogenetic relationships with other opisthorchiids

Mitochondrial genomic comparison of Clonorchis sinensis from South Korea with other isolates of this species

Genetic data of the ITS1-5.8S rDNA sequences of small liver flukes (Opisthorchiidae) from cats in Northern Vietnam

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