Genetic diversity of tea plants (Camellia sinensis (L.) Kuntze) growing in the Russian humid subtropics

Gvasaliya, M. V.

doi:10.31360/2225-3068-2018-66-28-34

Cited by 4 publications

(3 citation statements)

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“…Tea breeding was conducted here from 1950th and as the result many local cultivars were developed, such as Kolkhida, Qimen, Gruzinskii7, Karatum, Sochi , and many others. Also the set of mutant forms such as M#527, M#855, F#62, Clone #22 and many others were developed by UV and chemical mutagenesis (Gvasaliya, 2015 ). The genotypes included in our study characterized by high yield and quality in the local conditions.…”

Section: Discussionmentioning

confidence: 99%

“…Among them, Quimen, Gruzinskii7 were earlier showed to be the cold- and drought-tolerant genotypes; Kolkhida and Karatum were earlier showed to be cold-susceptible and drought-susceptible genotypes. Other clones and mutant forms showed medium cold-tolerance of drought-tolerance (Gvasaliya, 2015 ). Plants were grown in 2-liter pots filled with brown forest acidic soil (pH = 5.0) ( Figure 1 ).…”

Section: Methodsmentioning

confidence: 99%

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Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

et al. 2020

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Cold and drought are two of the most severe threats affecting the growth and productivity of the tea plant, limiting its global spread. Both stresses cause osmotic changes in the cells of the tea plant by decreasing their water potential. To develop cultivars that are tolerant to both stresses, it is essential to understand the genetic responses of tea plant to these two stresses, particularly in terms of the genes involved. In this study, we combined literature data with interspecific transcriptomic analyses (using Arabidopsis thaliana and Solanum lycopersicum) to choose genes related to cold tolerance. We identified 45 stress-inducible candidate genes associated with cold and drought responses in tea plants based on a comprehensive homologous detection method. Of these, nine were newly characterized by us, and 36 had previously been reported. The gene network analysis revealed upregulated expression in ICE1-related cluster of bHLH factors, HSP70/BAM5 connected genes (hexokinases, galactinol synthases, SnRK complex, etc.) indicating their possible co-expression. Using qRT-PCR we revealed that 10 genes were significantly upregulated in response to both cold and drought in tea plant: HSP70, GST, SUS1, DHN1, BMY5, bHLH102, GR-RBP3, ICE1, GOLS1, and GOLS3. SnRK1.2, HXK1/2, bHLH7/43/79/93 were specifically upregulated in cold, while RHL41, CAU1, Hydrolase22 were specifically upregulated in drought. Interestingly, the expression of CIP was higher in the recovery stage of both stresses, indicating its potentially important role in plant recovery after stress. In addition, some genes, such as DHN3, bHLH79, PEI54, SnRK1.2, SnRK1.3, and Hydrolase22, were significantly positively correlated between the cold and drought responses. CBF1, GOLS1, HXK2, and HXK3, by contrast, showed significantly negative correlations between the cold and drought responses. Our results provide valuable information and robust candidate genes for future functional analyses intended to improve the stress tolerance of the tea plant and other species.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

et al. 2020

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“…The experiments on cold and frost induction were carried out using two-year-old plants of tea cv. Kolkhida (frost sensitive) and Gruzinskii7 (frost tolerant) ( Tuov & Ryndin, 2011 ; Gvasaliya, 2015 ) ( Fig. 1 ).…”

Section: Methodsmentioning

confidence: 99%

Physiological, biochemical and genetic responses of Caucasian tea (Camellia sinensis(L.) Kuntze) genotypes under cold and frost stress

Самарина

Malyukova

Efremov

et al. 2020

PeerJ

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Background Cold and frost are two serious factors limiting the yield of many crops worldwide, including the tea plant (Camellia sinensis (L.) Kuntze). The acclimatization of tea plant from tropical to temperate climate regions resulted in unique germplasm in the North–Western Caucasus with extremely frost-tolerant genotypes. Methods The aim of the current research was to evaluate the physiological, biochemical and genetic responses of tolerant and sensitive tea cultivars exposed to cold (0 to +2 °C for 7 days) and frost (−6 to −8 °C for 5 days). Relative water content, cell membranes integrity, pH of the cell sap, water soluble protein, cations, sugars, amino acids were measured under cold and frost. Comparative expression of the following genes ICE1, CBF1, WRKY2, DHN1, DHN2, DHN3, NAC17, NAC26, NAC30, SnRK1.1, SnRK1.2, SnRK1.3, bHLH7, bHLH43, P5CS, LOX1, LOX6, LOX7 were analyzed. Results We found elevated protein (by 3–4 times) and cations (potassium, calcium and magnesium) contents in the leaves of both cultivars under cold and frost treatments. Meanwhile, Leu, Met, Val, Thr, Ser were increased under cold and frost, however tolerant cv. Gruzinskii7 showed earlier accumulation of these amino acids. Out of 18 studied genes, 11 were expressed at greater level in the frost- tolerant cultivar comparing with frost-sensitive one: ICE1, CBF1, WRKY2, DHN2, NAC17, NAC26, SnRK1.1, SnRK1.3, bHLH43, P5CS and LOX6. Positive correlations between certain amino acids namely, Met, Thr, Leu and Ser and studied genes were found. Taken together, the revealed cold responses in Caucasian tea cultivars help better understanding of tea tolerance to low temperature stress and role of revealed metabolites need to be further evaluated in different tea genotypes.

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About the Content of Pigments, Phenolic Compounds and Antiradical Activity of Young Tea Shoots (Camellia Sinensis L.)

Юрьевна¹,

Николаевна²,

Леонидовна³

et al. 2019

JCPRM

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The data on the morphophysiological characteristics of young three-leaf shoots (flashes, the first collection wave) of Camellia sinensis L. cultivars Kolhida and Kimyn, cultivated in the conditions of Russia subtropics (Krasnodar region, Sochi), the accumulation of photosynthetic pigments (chlorophylls a and b), phenolic compounds, including flavanes, and antiradical activity of extracts obtained from them are presented. It has been shown that the Kolhida cultivar is characterized by the formation of larger leaves compared to those of the Kimyn cultivar, as well as a high accumulation of photosynthetic pigments in them. At the same time, in the leaves of both tea representatives, the total content of chlorophylls a and b was higher compared with the stems. A similar tendency is characteristic for the accumulation of the phenolic compounds sum and flavanes, whereas the number of proanthocyanidins (soluble and insoluble) in the stems exceeded that in the leaves by 2–3 times. Both cultures had the same ability to form all forms of phenolic compounds, which is consistent with the data on the activity of L- phenylalanine ammonia-lyase – the key enzyme of their metabolism. It was established that the antiradical activity of extracts obtained from tea leaves was, on average, 30–40% higher than that of the stems and correlated with the content of phenolic compounds in them.

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Genetic diversity of tea plants (Camellia sinensis (L.) Kuntze) growing in the Russian humid subtropics

Cited by 4 publications

References 0 publications

Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

Comparative Expression Analysis of Stress-Inducible Candidate Genes in Response to Cold and Drought in Tea Plant [Camellia sinensis (L.) Kuntze]

Physiological, biochemical and genetic responses of Caucasian tea (Camellia sinensis(L.) Kuntze) genotypes under cold and frost stress

About the Content of Pigments, Phenolic Compounds and Antiradical Activity of Young Tea Shoots (Camellia Sinensis L.)

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