Genetic Diversity of Sweet Potato (Ipomoea batatas L. Lam) Germplasms Collected Worldwide Using Chloroplast SSR Markers

Abstract

Sweet potato (Ipomoea batatas L. Lam) is an important food crop widely cultivated in the world. In this study, nine chloroplast simple sequence repeat (cpSSR) markers were used to analyze the genetic diversity and relationships of 558 sweet potato accessions in the germplasm collection of the National Agrobiodiversity Center (NAC). Eight of the nine cpSSR showed polymorphisms, while Ibcp31 did not. The number of alleles per locus ranged from two to four. In general, the Shannon index for each cpSSR ranged from 0.280 to 1.123 and the diversity indices and unbiased diversity ranged from 0.148 to 0.626, and 0.210 to 0.627, respectively. Results of the median-joining network showed 33 chlorotypes in 558 sweet potato accessions. In factor analysis, 558 sweet potato accessions were divided into four clusters, with clusters I and II composed only of the sweet potato accessions from Korea, Japan, Taiwan, and the USA. The results of this study confirmed that the genetic diversity of the female parents of sweet potato accessions conserved at the NAC is low and therefore more sweet potato accessions need to be collected. These results will help to establish an efficient management plan for sweet potato genetic germplasms at the NAC.

Comparative analyses of 32 complete plastomes of Tef (Eragrostis tef ) accessions from Ethiopia: phylogenetic relationships and mutational hotspots

“…Most of the cpSSR in E. tef is distributed in the noncoding region of the genome, which is consistent with other studies (Li et al, 2018;Abdullah et al, 2019). Chloroplast derived microsatellite markers were developed and utilized in various studies such as assessment of the maternal line of hybrid wheat (Tomar et al, 2014), genetic diversity and relationships analysis among potato accessions (Lee et al, 2019) and species differentiation (Decesare, Hodkinson & Barth, 2010). Our study provides cpSSRs data that could provide valuable molecular tools for the evolutionary studies of E. tef.…”

Sustainable sweetpotato production in the United States: Current status, challenges, and opportunities

“…Evaluation: Sweetpotato breeding programs depend on the germplasm collection to improve nutritional quality, (Elameen et al, 2008), 197 accessions from China (Su et al, 2017), 303 and 604 accessions from the United States (Slonecki et al, 2023;Wadl et al, 2018), 558 accessions from Korea (Lee at al., 2019), and 5979 accessions from CIP (Anglin et al, 2021). Based on the number of accessions investigated, two, three, four, and six subpopulations were revealed, respectively.…”

“…Evaluation : Sweetpotato breeding programs depend on the germplasm collection to improve nutritional quality, disease/pest resistance, and other agronomic traits. To better utilize the sweetpotato germplasm collections, researchers have characterized the genetic diversity of global collections with various DNA marker platforms (AFLP, SLAF, GBSpoly, chSSR, and SSR) for 97 accessions from Tanzania (Elameen et al., 2008), 197 accessions from China (Su et al., 2017), 303 and 604 accessions from the United States (Slonecki et al., 2023; Wadl et al., 2018), 558 accessions from Korea (Lee at al., 2019), and 5979 accessions from CIP (Anglin et al., 2021). Based on the number of accessions investigated, two, three, four, and six subpopulations were revealed, respectively.…”

Analysis of Crop Genetic and Germplasm Diversity

“…The results of this study confirm that the genetic diversity of the female parents of sweet potato accessions conserved at the NAC is low, and therefore, more sweet potato accessions need to be collected. These results will help to establish an efficient management plan for sweet potato genetic germplasms at the NAC [21]. Sweet potato is also an important starch-producing crop, but little is known about the genetic variations in starch biosynthesis and sucrose metabolism genes.…”

“…Genetic variation and population structure and phytochemical and molecular diversity were studied in these crops. The variation in a relevant tuber crop, sweet potato (Ipomoea batatas L. Lam) [21,22], was assessed using chloroplast SSR markers and sequence diversity of biosynthesis pathways. In legumes, the genetic diversity in cowpea (Vigna unguiculata (L.) Walp.)…”